[1]黄愉菊,张剑勇.慈苓化浊方对痛风大鼠滑膜组织环氧化酶-2、热休克蛋白70表达的影响[J].陕西中医,2024,(8):1026-1030.[doi:DOI:10.3969/j.issn.1000-7369.2024.08.004]
　HUANG Yuju,ZHANG Jianyong.Effect of Ciling Huazhuo formula on the expression of COX-2 and HSP70 in synovial tissues of gout rats[J].,2024,(8):1026-1030.[doi:DOI:10.3969/j.issn.1000-7369.2024.08.004]
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慈苓化浊方对痛风大鼠滑膜组织环氧化酶-2、热休克蛋白70表达的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年8期
页码:
1026-1030
栏目:
基础研究
出版日期:
2024-08-05

文章信息/Info

Title:
Effect of Ciling Huazhuo formula on the expression of COX-2 and HSP70 in synovial tissues of gout rats
作者:
黄愉菊1; 张剑勇2
(1.广州中医药大学第四临床医学院,广东 深圳 518033; 2.深圳市中医院风湿病科,广东 深圳 518033)
Author(s):
HUANG Yuju; ZHANG Jianyong
(The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine,Shenzhen 518033,China)
关键词:
痛风;  慈苓化浊方;  环氧化酶-2;  热休克蛋白70;  滑膜;  大鼠
Keywords:
Gout;  Ciling Huazhuo formula;  Cyclooxygenase-2;  Heat shock protein 70;  Synovium;  Rats
分类号:
R 589.7
DOI:
DOI:10.3969/j.issn.1000-7369.2024.08.004
文献标志码:
A
摘要:
目的:分析慈苓化浊方对痛风大鼠滑膜组织中环氧化酶-2(COX-2)、热休克蛋白70(HSP70)表达的影响。方法:选取50只SPF级SD大鼠,10只大鼠作为对照组,另外40只建立痛风模型,将30只建模成功的大鼠分为模型组、药物对照组、慈苓化浊方组,每组10只。观察各组大鼠组织病理学、关节肿胀度、COX-2、HSP70表达、炎症因子、磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(AKT)/叉头框转录因子O亚族1(FoxO1)通路相关因子及其mRNA表达量。结果:与对照组相比,模型组、药物对照组、慈苓化浊方组关节肿胀度、COX-2、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)、PI3K mRNA、AKT mRNA、FoxO1 mRNA表达量升高,HSP70 mRNA、Caspase-3 mRNA表达量降低(均P<0.05)。与模型组相比,药物对照组、慈苓化浊方组关节肿胀度、COX-2、TNF-α、IL-1β、IL-6、PI3K mRNA、AKT mRNA、FoxO1 mRNA表达量降低,HSP70 mRNA、Caspase-3 mRNA表达量升高(均P<0.05)。与药物对照组相比,慈苓化浊方组关节肿胀度、COX-2、TNF-α、IL-1β、IL-6、PI3K mRNA、AKT mRNA、FoxO1 mRNA表达量降低,HSP70 mRNA、Caspase-3 mRNA表达量升高(均P<0.05)。结论:采用慈苓化浊方干预痛风大鼠,可降低大鼠关节肿胀程度,降低滑膜组织COX-2、HSP70表达,抑制炎症反应,其作用机制可能与抑制PI3K/AKT/FoxO1通路有关。
Abstract:
Objective:To analyze the effect of Ciling Huazhuo formula on the expression of cyclooxygenase-2(COX-2)and heat shock protein 70(HSP70)in synovium of gout rats.Methods:50 SPF grade SD rats were selected,with 10 rats as the control group and an additional 40 rats as the gout model.The 30 successfully modeled rats were divided into a model group,a drug control group,and a Ciling Huazhuo formula group,with 10 rats in each group.The pathological histology,joint swelling,expression of COX-2 and HSP70,inflammatory factors,phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/forkhead box transcription factor O subfamily 1(FoxO1)pathway related factors and their mRNA expression levels were observed in each group.Results:Compared with the control group,the expressions of joint swelling,COX-2,TNF-α,interleukin-1β(IL-1β),interleukin-6(IL-6),PI3K mRNA,AKT mRNA and FoxO1 mRNA in model group,drug control group and Ciling Huazhuo formula group were increased,the expression levels of HSP70 mRNA and Caspase-3 mRNA were decreased(all P<0.05).Compared with model group,the expressions of joint swelling,COX-2,TNF-α,IL-1β,IL-6,PI3K mRNA,AKT mRNA and FoxO1 mRNA in drug control group and Ciling Huazhuo formula group decreased,while the expressions of HSP70 mRNA and Caspase-3 mRNA increased(all P<0.05).Compared with drug control group,the expressions of joint swelling,COX-2,TNF-α,IL-1β,IL-6,PI3K mRNA,AKT mRNA and FoxO1 mRNA in Ciling Huazhuo formula group were decreased,while the expressions of HSP70 mRNA and Caspase-3 mRNA were increased(all P<0.05).Conclusion:The intervention of Ciling Huazhuo decoction in gout rats can reduce the degree of joint swelling,reduce the expression of COX-2 and HSP70 in synovial tissue,and inhibit the inflammatory response.The mechanism of action may be related to the inhibition of PI3K/AKT/FoxO1 pathway.

参考文献/References:

[1] ASHIQ K,BAIDA M A,TANVEER S,et al.A comprehensive review on gout:The epidemiological trends,pathophysiology,clinical presentation,diagnosis and treatment[J].J Pak Med Assoc,2021,71(4):1234-1238.
[2] YOKOSE C,MCCORMICK N,CHOI H K.Dietary and lifestyle-centered approach in gout care and prevention[J].Curr Rheumatol Rep,2021,23(7):51.
[3] 贾二涛,姜毓楷,何思慧,等.慈苓化浊颗粒对痛风患者肠道微生物的影响[J].广州中医药大学学报,2022,39(3):522-526.
[4] 罗莉,张光对,林远瑶,等.高频超声联合UA、CRP及COX-2检测在痛风性关节炎诊断中的应用分析[J].解放军医药杂志,2022,34(7):94-97.
[5] 张俊,李雪锋,张普.血清铁蛋白、P2X7R、HSP70在痛风患者中的表达及其临床价值[J].临床和实验医学杂志,2021,20(23):2535-2538.
[6] 郭玉星,朱珊莹,康佩芝,等.AQP2、HSP70在痛风湿热蕴结证模型中表达的意义[J].世界科学技术-中医药现代化,2020,22(11):3894-3899.
[7] BRZEZINSKA O,STYRZYNSKI F,MAKOWSKA J,et al.Role of vitamin c in prophylaxis and treatment of gout-a literature review[J].Nutrients,2021,13(2):701.
[8] 单兆亚,王波.右美托咪定调控急性痛风性关节炎模型大鼠血清炎性因子水平及滑膜功能效果研究[J].陕西医学杂志,2021,50(9):1052-1055.
[9] 庞利君,黄传兵,李明,等.清热利湿除痹方治疗急性痛风性关节炎患者的疗效及对炎症因子的影响[J].中国中医急症,2023,32(11):1932-1936.
[10] ECKENSTALER R,BENNDORF R A.The role of ABCG2 in the pathogenesis of primary hyperuricemia and gout-an update[J].Int J Mol Sci,2021,22(13):6678.
[11] 匡志平,陈洪,赵忠胜,等.肿意膏治疗膝关节急性痛风性关节炎的疗效观察[J].中国中医急症,2023,32(11):1980-1982.
[12] 李泽宇,郝二伟,曹瑞,等.基于数据挖掘和网络药理学分析中药治疗痛风的用药规律及作用机制[J].科学技术与工程,2022,22(36):15967-15979.
[13] 朱琳,陈鹏,孙玄静,等.血清、滑液中IL-1β和COX-2水平在痛风性关节炎中的动态变化及临床意义[J].国际内分泌代谢杂志,2021,41(3):168-172.
[14] 李明,王东明.关节镜微创术联合补肾利湿法对膝关节急性痛风性关节炎患者血清UA、ESR、COX-2水平的影响[J].河北医学,2020,26(12):2061-2065.
[15] 亓国锋,楚天舒,刘谓,等.加味四妙汤辅助治疗痛风性关节炎对RAGE、COX-2水平变化研究[J].中华中医药学刊,2021,39(1):11-13.
[16] 黄玉梅,罗静思,曹晓兰,等.PCOS患者血清HSP70、炎症-氧化应激指标表达及与胰岛素抵抗的关系[J].中国妇产科临床杂志,2020,21(3):295-296.
[17] 王慧渊,李静,耿妍,等.CpG ODN联合HSP70/CD80DNA疫苗对哮喘小鼠血清IL-4、IL-13、IFN-γ及支气管肺泡灌洗液中IL-4、IL-25、1L-33的水平变化影响[J].中国儿童保健杂志,2020,28(12):1338-1342.
[18] 夏顺杰,李钊希,王琨璐,等.Hsf1通过HSP70-TLR4-MyD88信号通路调控肾脏AA淀粉变[J].临床与实验病理学杂志,2021,37(12):1440-1445.
[19] 南晓强,杨浩峰,雷鹏.息痛散通过富含半胱氨酸蛋白61对痛风性关节炎大鼠炎症反应的干预机制[J].陕西中医,2022,43(12):1659-1663.
[20] 肖艳,毛骁,蔡唐彦,等.痛风宁含药血清对尿酸盐刺激的THP-1炎症反应的时效观察[J].世界中医药,2021,16(21):3191-3197.
[21] 郭良,谭昱,马春涛,等.四妙散加减治疗湿热蕴结型急性痛风性关节炎疗效及对患者T细胞亚群、血液流变学及炎性标志物的影响[J].陕西中医,2023,44(2):199-204.
[22] 梁再赋,索德宝,冯晓明,等.雌性红莱菔对急性痛风性关节炎模型大鼠抗炎作用及激活TLR/MyD88/IL-1β信号通路作用机制的研究[J].风湿病与关节炎,2022,11(4):1-6.
[23] 李芨慧,李玉宏,葛丽丽.急性痛风性关节炎患者肌骨超声半定量评分与红细胞沉降率、白细胞介素-6水平及疾病活动度相关性分析[J].陕西医学杂志,2022,51(3):318-321.
[24] XIANG M,LIU T,TIAN C,et al.Kinsenoside attenuates liver fibro-inflammation by suppressing dendritic cells via the PI3K-AKT-FoxO1 pathway[J].Pharmacol Res,2022,177(8):10-16.
[25] 王峰,张安兵.基于PI3K/AKT/FoxO1信号通路探究补阳还五汤对痛风模型大鼠滑膜组织细胞凋亡及炎症反应的影响[J].免疫学杂志,2022,38(7):612-619.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助面上项目(82174290)
更新日期/Last Update: 2024-08-08