[1]刘 清,王 芳,张淑娟,等.白虎加人参汤对2型糖尿病大鼠抗氧化应激作用及机制研究[J].陕西中医,2024,(9):1166-1171.[doi:DOI:10.3969/j.issn.1000-7369.2024.09.003]
　LIU Qing,WANG Fang,ZHANG Shujuan,et al.Effect and mechanism of Baihu jia Renshen decoction on anti-oxidative stress in type 2 diabetic rats[J].,2024,(9):1166-1171.[doi:DOI:10.3969/j.issn.1000-7369.2024.09.003]
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白虎加人参汤对2型糖尿病大鼠抗氧化应激作用及机制研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年9期
页码:
1166-1171
栏目:
基础研究
出版日期:
2024-09-05

文章信息/Info

Title:
Effect and mechanism of Baihu jia Renshen decoction on anti-oxidative stress in type 2 diabetic rats
作者:
刘 清1; 王 芳2;  张淑娟2;  RASIKA Gunarathne3; 严军虎4
(1.陕西中医药大学,陕西 咸阳 712046; 2.陕西能源职业技术学院, 陕西 咸阳 712000; 3.Auckland Bioengineering Institute,University of Auckland,Private Bag 92019,Auckland 1142,New Zealand; 4.陕西师范大学医院,陕西 西安 710062)
Author(s):
LIU Qing; WANG Fang; ZHANG Shujuan; RASIKA Gunarathne; YAN Junhu
(Shaanxi University of Chinese Medicine,Xianyang 712046,China)
关键词:
2型糖尿病;  白虎加人参汤;  抗氧化应激;  胰岛素抵抗;  胰岛β细胞
Keywords:
Type 2 diabetic;  Baihu jia Renshen decoction;  Antioxidant stress;  Insulin resistance;  Islet β cells
分类号:
R 587.1
DOI:
DOI:10.3969/j.issn.1000-7369.2024.09.003
文献标志码:
A
摘要:
目的:研究白虎加人参汤(BHJRS)对2型糖尿病(T2DM)模型大鼠抗氧化应激的作用,并初步探讨其作用机制。方法:随机取8只SD大鼠作为对照组(NC组),其余SD大鼠采用高脂高糖联合低剂量腹腔注射STZ(45 mg/kg)制备T2DM大鼠模型。将成模大鼠随机分为模型(DM)组、阳性药[盐酸二甲双胍(Met),200 mg/kg]组、BHJRS 高剂量组(BHJRS-H,BHJRS溶液37.8 g/kg)、BHJRS低剂量组(BHJRS-L,BHJRS溶液18.9 g/kg),连续给药8周。测定各组大鼠第0、2、4、6、8周空腹血糖(FBG),检测糖化血红蛋白(HbAlc)、胰岛素(INS)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)水平并计算ISI值,观察胰腺组织病理学形态变化; 检测胰腺组织中B淋巴细胞瘤-2基因(Bcl-2)、胱天蛋白酶3(Caspase-3)、Bcl-2相关X蛋白(Bax)的mRNA和蛋白水平; 检测胰腺组织中蛋白激酶B(AKT)/糖原合成激酶-3β(GSK-3β)/核因子NF-E2相关因子(Nrf2)信号通路关键蛋白的表达。结果:与DM组比较,Met 组、BHJRS-H组和BHJRS-L组显著降低T2DM大鼠FBG、血清中HbAlc、MDA水平(P<0.05),显著升高血清中INS、CAT、SOD、GSH-Px水平和 ISI值(P<0.05); 显著上调胰腺组织中p-AKT/AKT、p-GSK-3β/GSK-3β、n-Nrf2、血红素加氧酶-1(HO-1)水平(P<0.05),显著下调Bax/Bcl-2、Caspase-3、n-Fyn水平(P<0.05),胰腺组织病理变化均不同程度改善。结论:BHJRS对胰腺组织有一定的抗氧化应激作用,其原因可能在于调节AKT/GSK-3β/Nrf2信号通路中相关蛋白的表达有关。
Abstract:
Objective:To study the effect and mechanism of Baihu jia Renshen decoction(BHJRS)on oxidative stress in Type 2 diabetic rats,and to explore its mechanism.Methods:Eight SD rats were randomly selected as the control group(NC group),and the remaining SD rats were treated with high fat and high sugar combined with low dose of STZ(45 mg/kg)intraperitoneally to create T2DM rat model.The model rats were randomly divided into a model(DM)group,a positive drug [metformin(Met),200 mg/kg group],a BHJRS high-dose group(BHJRS-H,37.8 g/kg),a BHJRS low-dose group(BHJRS-L,18.9 g/kg).The drug was administered continuously for 8 weeks.FBG was measured at the 0,2,4,6 and 8 weeks in each group,and the levels of HbAlc,INS,MDA,GSH-Px,SOD and CAT were detected,and ISI values were calculated.The morphological changes of pancreatic histopathology were observed.The mRNA and protein levels of Bcl-2,Bax and Caspase-3 were detected in pancreatic tissue.The expression of AKT/GSK-3β/Nrf2 signaling pathway in pancreatic tissue was detected.Results:Compared with DM group,the levels of FBG,serum HbAlc and MDA in Met group,BHJRS-H group and BHJRS-L group were significantly decreased(P<0.05),and the levels of INS,CAT,SOD,GSH-Px and ISI in serum were significantly increased(P<0.05).Significantly increased the p-Akt /AKT ratio,p-GSK-3β/GSK-3β ratio,n-Nrf2 and heme oxygenase-1(HO-1)levels in pancreatic tissue(P<0.05),and significantly decreased the Bax/Bcl-2 ratio,Caspase-3 and n-Fyn levels(P<0.05).The histopathologic changes of pancreas were improved in different degree.Conclusion:BHJRS has a certain effect on the anti-oxidative stress of pancreatic tissue,and its mechanism may be related to the regulation of the expression of related proteins in the AKT/GSK-3β/Nrf2 signaling pathway.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(82374077); 陕西省自然科学基础研究计划项目(2021IQ-888); 陕西省教育厅科研计划项目(21JK0578)
更新日期/Last Update: 2024-09-09