[1]宋晨阳,孟庆良.基于PI3K/AKT信号通路探讨补肾壮骨汤对骨关节炎防治作用的实验研究[J].陕西中医,2024,(10):1311-1314,1319.[doi:DOI:10.3969/j.issn.1000-7369.2024.10.003]
　SONG Chenyang,MENG Qingliang.Experimental study on the preventive and therapeutic effects of Bushen Zhuanggu decoction on osteoarthritis based on the PI3K/AKT signaling pathway[J].,2024,(10):1311-1314,1319.[doi:DOI:10.3969/j.issn.1000-7369.2024.10.003]
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基于PI3K/AKT信号通路探讨补肾壮骨汤对骨关节炎防治作用的实验研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年10期
页码:
1311-1314,1319
栏目:
基础研究
出版日期:
2024-10-05

文章信息/Info

Title:
Experimental study on the preventive and therapeutic effects of Bushen Zhuanggu decoction on osteoarthritis based on the PI3K/AKT signaling pathway
作者:
宋晨阳; 孟庆良
(河南中医药大学骨伤学院,河南 郑州 450002)
Author(s):
SONG Chenyang; MENG Qingliang
(Academy of Orthopedics and Traumatology,Henan University of Chinese Medicine,Zhengzhou 450002,China)
关键词:
骨关节炎;  补肾壮骨汤;  PI3K/AKT信号通路;  软骨细胞;  增殖和凋亡;  抗炎;  抗凋亡基因B淋巴细胞瘤-2
Keywords:
Osteoarthritis;  Bushen Zhuanggu decoction;  PI3K/AKT signaling pathway;  Chondrocytes;  Proliferation and apoptosis;  Anti-inflammatory role;  Antiapoptotic gene B lymphoblastoma-2
分类号:
R 684.3
DOI:
DOI:10.3969/j.issn.1000-7369.2024.10.003
文献标志码:
A
摘要:
目的:基于PI3K/AKT信号通路探讨补肾壮骨汤对骨关节炎防治作用的机制。方法:取雄性SD大鼠30只,随机分为对照组、模型组和补肾壮骨组。其中对照组和模型组均给予等体积0.9%氯化钠溶液灌胃; 而补肾壮骨组给予补肾壮骨汤灌胃,连续给药4周。干预完成后处死大鼠,采集血液并取关节软骨组织。采用酶联免疫吸附测定(ELISA)检测血清中白细胞介素(IL)-1β、IL-6和IL-10的含量。采用实时定量聚合酶链反应(RT-qPCR)检测磷脂酰肌醇3激酶(PI3K)、丝氨酸-苏氨酸激酶(AKT)和哺乳动物雷帕霉素靶蛋白(mTOR)基因表达; 采用Western blot检测抗凋亡基因B淋巴细胞瘤-2(Bcl-2)和Survivin蛋白、促凋亡基因Bcl-2相关X蛋白(Bax)表达。结果:与对照组比较,模型组的软骨细胞活性明显降低; IL-1β和IL-6的含量均明显升高,而IL-10的含量显著下降; PI3K、AKT和mTOR基因和蛋白表达水平均显著降低; Bcl-2和Survivin蛋白表达增加,Bax蛋白表达降低(均P<0.05); 而与模型组比较,补肾壮骨组的软骨细胞活性明显增加; IL-1β和IL-6的含量明显下降,而IL-10的含量显著上升; PI3K、AKT和mTOR基因和蛋白表达水平均显著升高; Bcl-2和Survivin蛋白表达下降,Bax蛋白表达上升(均P<0.05)。结论:补肾壮骨汤有助于促进OA软骨细胞增殖、抑制软骨细胞凋亡,发挥抗炎作用,其机制可能与调控PI3K/AKT通路,进而介导软骨细胞增殖凋亡和抗炎作用有关。
Abstract:
Objective:To explore the on the preventive and therapeutic effects of Bushen Zhuanggu decoction on osteoarthritis based on the PI3K/AKT signaling pathway.Methods:A total of 30 male SD rats were randomly divided into a control group,a model group and a Bushen Zhuanggu group.Both the control group and the model group were given equal volumes of physiological saline by gavage.The Bushen Zhuanggu group was given Bushen Zhuanggu decoction by gavage for 4 consecutive weeks.After intervention,the rats were euthanized,blood was collected,and articular cartilage tissue was taken.Enzyme linked immunosorbent assay(ELISA)was used to detect the levels of interleukin(IL)-1β,IL-6,and IL-10 in rat serum.Real-time quantitative polymerase chain reaction(RT-qPCR)was applied to detect the gene expressions of phosphatidylinositol 3-kimase(PI3K),v-akt murine thymoma viral oncogene homolog(AKT),and mammalian target of rapamycin(mTOR); while Western blot was employed to detect the protein expressions of PI3K,AKT and mTOR,as well as antiapoptotic gene B lymphoblastoma-2(Bcl-2),Survivin,and proapoptotic gene Bcl-2-associated X protein(Bax).Results:Compared with the control group,the model group had significantly increased chondrocyte activity,obviously increased IL-1β and IL-6 contents,but remarkably decreased IL-10 content; as well as evidently downregulated gene and protein expressions of PI3K,AKT and mTOR; as well as increased Bcl-2 and Survivin protein expressions,and decreased bax protein expression(all P<0.05).While compared with the model group,Bushen Zhuanggu group showed significantly increased chondrocyte activity,obviously reduced IL-1β and IL-6 contents,but remarkably elevated IL-10 content; evidently upregulated gene and protein expressions of PI3K,AKT and mTOR; as well as reduced Bcl-2 and Survivin protein expressions,and elevated Bax protein expression(all P<0.05).Conclusion:Bushen Zhuanggu decoction can promote the proliferation but inhibit the apoptosis of chondrocytes in osteoarthritis,and inhibit inflammatory reactions,which may be related to mediating the activation of the PI3K/AKT pathway to participate in the regulation of chondrocyte proliferation,apoptosis,and inflammation.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81874456); 河南省中医药科学研究专项课题(20-21ZYZD05,2019ZY1018,2019ZY2032)
更新日期/Last Update: 2024-10-08