[1]杨 帆,魏小果,李 娟,等.清肝降脂汤对体外诱导非酒精性脂肪肝细胞模型脂代谢和过氧化物酶体增殖物激活受体-α的调节作用研究[J].陕西中医,2024,(10):1326-1329,1335.[doi:DOI:10.3969/j.issn.1000-7369.2024.10.006]
　YANG Fan,WEI Xiaoguo,LI Juan,et al.The regulatory effect of Qinggan Jiangzhi decoction on lipid metabolism and PPAR-α in non-alcoholic fatty liver cell model induced in vitro[J].,2024,(10):1326-1329,1335.[doi:DOI:10.3969/j.issn.1000-7369.2024.10.006]
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清肝降脂汤对体外诱导非酒精性脂肪肝细胞模型脂代谢和过氧化物酶体增殖物激活受体-α的调节作用研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年10期
页码:
1326-1329,1335
栏目:
基础研究
出版日期:
2024-10-05

文章信息/Info

Title:
The regulatory effect of Qinggan Jiangzhi decoction on lipid metabolism and PPAR-α in non-alcoholic fatty liver cell model induced in vitro
作者:
杨 帆; 魏小果; 李 娟; 何 昉; 李昌金
(甘肃省人民医院消化科,甘肃 兰州 730000)
Author(s):
YANG Fan; WEI Xiaoguo; LI Juan; HE Fang; LI Changjin
(Department of Gastroenterology,Gansu Provincial People's Hospital,Lanzhou 730000,China)
关键词:
非酒精性脂肪肝;  清肝降脂汤;  过氧化物酶体增殖物激活受体;  细胞模型;  脂代谢;  成纤维细胞生长因子-21
Keywords:
Non-alcoholic fatty liver disease;  Qinggan Jiangzhi decoction;  Peroxisome proliferator-activated receptor;  Cell model;  Lipid metabolism;  Fibroblast growth factor-21
分类号:
R 575.5
DOI:
DOI:10.3969/j.issn.1000-7369.2024.10.006
文献标志码:
A
摘要:
目的:探讨清肝降脂汤对体外诱导非酒精性脂肪肝(NAFLD)细胞模型脂代谢和过氧化物酶体增殖物激活受体(PPAR)-α的调节作用。方法:取HepG2细胞株,采用油酸构建NAFLD细胞模型。将细胞分为正常对照组(无油酸建模+无药物处理)、模型对照组(有油酸建模+无药物处理)和清肝降脂汤组(有油酸建模+有药物处理)。采用CCK-8实验检测细胞活性。采用油红O染色定性定量检测清肝降脂汤对细胞内脂质的影响,定量计算脂质含量的抑制率。同时,测定细胞内丙二醛(MDA)、超氧化物歧化酶(SOD)、甘油三酯(TG)、总胆固醇(TC)和游离脂肪酸(FFA)含量; 并采用聚合酶链反应(PCR)和Western blot检测脂肪代谢标志物PPAR-α和成纤维细胞生长因子-21(FGF21)的表达水平。结果:与正常对照组相比,模型对照组的HepG2细胞活性显著增高,脂质含量显著增高,MDA、SOD、TG、TC和FFA含量显著增高,而PPAR-α和FGF21表达水平显著降低(均P<0.05); 而与模型对照组相比,清肝降脂汤组的HepG2细胞活性显著降低,脂质含量显著降低,MDA、SOD、TG、TC和FFA含量显著降低,而PPAR-α和FGF21表达水平显著增高(均P<0.05)。结论:清肝降脂汤有助于降低HepG2细胞活性、脂质含量以及MDA、SOD、TG、TC和FFA含量,并促进PPAR-α和FGF21表达,进而调节脂质代谢,抑制NAFLD发展进程。
Abstract:
Objective:To clarify the regulatory effects of Qinggan Jiangzhi decoction on lipid metabolism and peroxisome proliferator-activated receptor(PPAR)-α in non-alcoholic fatty liver(NAFLD)cell model induced in vitro.Methods:HepG2 cell line was used to construct NAFLD cell model using oleic acid.The grouping scheme was described as follows:normal control group(without oleic acid modeling+drug treatment),model control group(with oleic acid modeling+drug treatment),and Qinggan Jiangzhi decoction group(with oleic acid modeling+drug treatment).Cell activity was detected using CCK-8 assay.Qualitative and quantitative detection of the effect of Qinggan Jiangzhi decoction on intracellular lipids was performed using oil red O staining,and the inhibition rate of lipid content was quantitatively calculated.Meanwhile,the intracellular malondialdehyde(MDA),superoxide dismutase(SOD),triglycerides(TG),total cholesterol(TC),and free fatty acids(FFA)contents were measured at the same time.PCR and Western blot was used to detect the expressions of fat metabolism marker PPAR-α and fibroblast growth factor-21(FGF21).Results:Compared with the normal control group,the model control group showed a significant increase in HepG2 cell activity,lipid content,MDA,SOD,TG,TC and FFA contents,and significant decrease in expressions of PPAR-α and FGF21(all P<0.05).While compared with the model control group,the HepG2 cell activity,lipid content as well as MDA,SOD,TG,TC and FFA contents of the Qinggan Jiangzhi decoction group were significantly reduced,and PPAR-α and FGF21 expressions were significantly increased(all P<0.05).Conclusion:Qinggan Jiangzhi decoction helps to reduce HepG2 cell activity,lipid content,and MDA,SOD,TG,TC and FFA contents,and promotes PPAR-α and FGF21 expressions to regulate lipid metabolism and inhibit the development process of NAFLD.

参考文献/References:

[1] 郑文博,赵瑛.冬凌草甲素对高脂饮食诱导幼龄大鼠非酒精性脂肪肝的作用及相关机制[J].陕西医学杂志,2021,50(12):1496-1500.
[2] DANPANICHKUL P,NG C H,MUTHIAH M D,et al.The silent burden of non-alcoholic fatty liver disease in the elderly:A global burden of disease analysis[J].Aliment Pharmacol Ther,2023,58(10):1062-1074.
[3] 董文娟,杨立宏,张文佳.降脂瘦身汤对非酒精性脂肪肝大鼠脂代谢及核转录因子-κB蛋白表达影响的实验研究[J].陕西中医,2021,42(8):1016-1019.
[4] 李怡萍,杨丽丽,郑昱,等.三子养亲汤对非酒精性脂肪性肝病小鼠脂质代谢紊乱的调控作用[J].中成药,2022,44(3):913-917.
[5] YOUNOSSI Z,ANSTEE Q M,MARIETTI M,et al.Global burden of NAFLD and NASH:Trends,predictions,risk factors and prevention[J].Nat Rev Gastroenterol Hepatol,2018,15(1):11-20.
[6] 刘锦成.清肝利湿降脂汤治疗酒精性脂肪肝疗效观察[J].实用中医药杂志,2018,34(4):412-413.
[7] WANG C,LV Z L,KANG Y J,et al.Aquaporin-9 downregulation prevents steatosis in oleic acid-induced non-alcoholic fatty liver disease cell models[J].Int J Mol Med,2013,32(5):1159-1165.
[8] TOROUS V F,BRACKETT D,BROWN P,et al.Oil red O staining for lipid-laden macrophage index of bronchoalveolar lavage:Interobserver agreement and challenges to interpretation[J].J Am Soc Cytopathol,2020,9(6):563-569.
[9] ZHOU J,ZHOU F,WANG W,et al.Epidemiological features of nafld from 1999 to 2018 in China[J].Hepatology,2020,71(5):1851-1864.
[10] YIP T C,LEE H W,CHAN W K,et al.Asian perspective on NAFLD-associated HCC[J].J Hepatol,2022,76(3):726-734.
[11] TARGHER G,BYRNE C D,TILG H.NAFLD and increased risk of cardiovascular disease:Clinical associations,pathophysiological mechanisms and pharmacological implications[J].Gut,2020,69(9):1691-1705.
[12] TIE F,DING J,HU N,et al.Kaempferol and kaempferide attenuate oleic acid-induced lipid accumulation and oxidative stress in HepG2 cells[J].Int J Mol Sci,2021,22(16):8847.
[13] 李莎,寇少杰,薛敬东.薛敬东运用“浊毒理论”治疗非酒精性脂肪肝经验总结[J].陕西中医,2021,42(3):363-365.
[14] 王素格,李德征,蒋树林,等.高脂饮食脂肪肝胰岛素抵抗大鼠中PPARα的表达对肝组织的影响[J].河北医药,2013,35(6):810-812.
[15] 王玲,赵健亚,刘宗平.TLR4/MyD88通路在油酸促进BRL3A细胞脂肪变性中的作用[J].中国兽医科学,2020,50(1):112-117.
[16] ANWAR H,SUCHODOLSKI J S,ULLAH M I,et al.Shiitake culinary-medicinal mushroom,lentinus edodes(agaricomycetes),supplementation alters gut microbiome and corrects dyslipidemia in rats[J].Int J Med Mushrooms,2019,21(1):79-88.
[17] 刘应莉,王艳荣,张秋瓒.肠道菌群-过氧化物酶体增殖物激活受体信号通路及其在代谢相关脂肪性肝病发病中的作用研究进展[J].陕西医学杂志,2021,50(9):1166-1169.
[18] SAXENA P,SELVARAJ K,KHARE S K,et al.Superoxide dismutase as multipotent therapeutic antioxidant enzyme:Role in human diseases[J].Biotechnol Lett,2022,44(1):1-22.
[19] BOUGARNE N,WEYERS B,DESMET S J,et al.Molecular actions of PPARα in lipid metabolism and inflammation[J].Endocr Rev,2018,39(5):760-802.
[20] TAKADA I,MAKISHIMA M.Peroxisome proliferator-activated receptor agonists and antagonists:A patent review(2014-present)[J].Expert Opin Ther Pat,2020,30(1):1-13.
[21] LEE B,AN H J,KIM D H,et al.SMP30-mediated synthesis of vitamin C activates the liver PPARα/FGF21 axis to regulate thermogenesis in mice[J].Exp Mol Med,2022,54(11):2036-2046.
[22] NAKAGAWA Y,SHIMANO H.CREBH regulates systemic glucose and lipid metabolism[J].Int J Mol Sci,2018,19(5):1396-1410.
[23] QIU H,SONG E,HU Y,et al.Hepatocyte-secreted autotaxin exacerbates nonalcoholic fatty liver disease through autocrine inhibition of the PPARα/FGF21 axis[J].Cell Mol Gastroenterol Hepatol,2022,14(5):1003-1023.
[24] 唐允婷,陈宇彬,吴小文,等.非酒精性脂肪肝与单纯性肥胖中医治疗方剂用药规律对比分析[J].浙江中医药大学学报,2023,47(7):804-812.
[25] 朱雪萍,张丽梅,毛信心,等.丹参川芎嗪注射液对心绞痛疗效及血脂影响的Meta分析[J].中国现代应用药学,2022,39(5):638-647.
[26] 李松涛,葛岚岚,肖凌云,等.金银花化学成分的抗病毒作用研究进展[J].新发传染病电子杂志,2020,5(2):136-139.
[27] 刘玉玲,汪栋材,陈博勉,等.王孟庸治疗绝经后女性反复发作尿路感染经验[J].西部中医药,2020,33(11):53-56.

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备注/Memo

备注/Memo:
基金项目:甘肃省科技计划项目(21JR1RA032)
更新日期/Last Update: 2024-10-08