[1]邓文芸,黄雅静,申 震.泽兰合剂对肢体缺血再灌注损伤大鼠炎性损伤的作用[J].陕西中医,2024,(11):1460-1465.[doi:DOI:10.3969/j.issn.1000-7369.2024.11.004]
　DENG Wenyun,HUANG Yajing,SHEN Zhen.Effects of Zelan mixture on inflammatory damage of rats with limb ischemia-reperfusion injury[J].,2024,(11):1460-1465.[doi:DOI:10.3969/j.issn.1000-7369.2024.11.004]
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泽兰合剂对肢体缺血再灌注损伤大鼠炎性损伤的作用
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年11期
页码:
1460-1465
栏目:
基础研究
出版日期:
2024-11-05

文章信息/Info

Title:
Effects of Zelan mixture on inflammatory damage of rats with limb ischemia-reperfusion injury
作者:
邓文芸; 黄雅静; 申 震
(云南中医药大学第三附属医院,云南 昆明 650599)
Author(s):
DENG Wenyun; HUANG Yajing; SHEN Zhen
(The Third Affiliated Hospital of Yunnan University of Traditional Chinese Medicine,Kunming 650599,China)
关键词:
肢体缺血再灌注损伤;  泽兰合剂;  骨骼肌;  炎性损伤;  肿瘤坏死因子-α;  高迁移率族蛋白B1
Keywords:
Limb ischemia-reperfusion injury;  Zelan mixture;  Skeletal muscle;  Inflammatory damage;  TNF-α;  HMGB1
分类号:
R 289
DOI:
DOI:10.3969/j.issn.1000-7369.2024.11.004
文献标志码:
A
摘要:
目的:观察泽兰合剂对肢体缺血再灌注损伤(LIRI)模型大鼠TNF-α、HMGB1表达的影响,探讨泽兰合剂对LIRI炎性损伤的治疗作用。方法:75只雄性SD大鼠,按随机数字表分为五组,对照组、模型组、泽兰合剂低剂量组、泽兰合剂中剂量组、泽兰合剂高剂量组,每组15只,其中按再灌注后不同时间点分为0、2、6 h 3个时间亚组,每组5只。造模后HE染色观察骨骼肌病理组织形态,免疫荧光染色检测骨骼肌肿瘤坏死因子-α(TNF-α)、高迁移率族蛋白B1(HMGB1)表达。ELISA检测血清TNF-α、HMGB1水平变化,RT-qPCR检测骨骼肌中TNF-α、HMGB1的mRNA表达。结果:模型组、泽兰合剂低剂量组、泽兰合剂中剂量组、泽兰合剂高剂量组TNF-α、HMGB1表达较对照组升高,差异有统计学意义(均P<0.05)。泽兰合剂低剂量组、泽兰合剂中剂量组TNF-α、HMGB1含量在相同时间点较模型组降低,其中泽兰合剂中剂量组水平最低,而泽兰合剂高剂量组HMGB1、TNF-α在同时间点较模型组升高,差异有统计学意义(均P<0.05)。结论:中低剂量泽兰合剂均能降低缺血再灌注损伤大鼠TNF-α、HMGB1水平,减轻炎症损伤,且以中剂量泽兰合剂效果最佳,高剂量泽兰合剂则会加剧炎性损伤。
Abstract:
Objective:To observe treatment effects of inflammatory factors of rats with limb ischemia-reperfusion injury(LIRI),and investigate treatment of Zelan mixture for LIRI.Methods:Seventy-five male rats were randomly divided into five groups,control group,model group,low doses of Zelan mixture group,medium doses of Zelan mixture group and high doses of Zelan mixture group,15 rats in each group.Based on the time points of 0 h,2 h and 6 h,subgroups were carried out 5 rats in each group.After establishing limb ischemia reperfusion injury model,the pathological tissue morphology of skeletal muscles were observed by HE staining.The expression of TNF-α,HMGB1 in skeletal muscles were detected by immunofluorescence staining.The expression levels of TNF-α and HMGB1 in serum by ELISA and mRNA levels of TNF-α and HMGB1 in skeletal muscle tissues by RT-qPCR were carried out.Results:The expression of TNF-α and HMGB1 in the model group,the Zelan mixture low dose group,Zelan mixture medium dose group and Zelan mixture high dose group were higher than those in the control group,difference statistically significant(all P<0.05).The contents of TNF-α and HMGB1 in the Zelan mixture low dose group and Zelan mixture medium dose group were lower than those in the model group at the same time point,and levels of HMGB1 and TNF-α in the Zelan mixture high dose group were higher than those in model group at the same time point,with statistical significance(all P<0.05).Conclusion:The levels of TNF-α and HMGB1 in rats with ischemia-reperfusion injury could be reduced by Zelan mixture at medium and low dose,and medium dose Zelan mixture was better.High dose Zelan mixture can aggravate inflammatory injury.

参考文献/References:

[1] 竹亮,李春卉,谢生茂.骨骼肌缺血再灌注损伤的影响因素和临床相关疾病[J].创伤外科杂志,2020,22(9):709-712.
[2] 刘宗义,朱付平,李武平.桃红四物汤对大鼠肢体缺血-再灌注损伤骨骼肌长链非编码RNA H19、核因子κB-p65表达的影响[J].陕西中医,2022,43(9):1163-1169.
[3] 刁长会,刘修恒.缺血再灌注损伤的病理生理机制[J].医学综述,2018,24(12):2306-2311,2317.
[4] 张俊辉,郑志鹏,何成龙,等.关于肢体缺血再灌注损伤评价指标的研究进展[J].广东药科大学学报,2019,35(1):153-157.
[5] 胡敏,於四军.褪黑素对大鼠挤压综合征模型骨骼肌缺血再灌注损伤的影响[J].临床研究,2021,29(2):1-4.
[6] 李瑞华,阙世廉,李津.骨骼肌缺血再灌注损伤研究进展[J].中国中西医结合外科杂志,2017,23(4):459-461.
[7] 闫斌,哈斯鲁,买合木提?亚库甫,等.止血带致肢体缺血再灌注损伤时炎性递质变化及缺血预处理的作用[J].中国组织工程研究,2015,19(46):7445-7450.
[8] 申震,朱付平,刘天举,等.桃红四物液对大鼠肢体缺血-再灌注损伤炎症因子表达的影响研究[J].中华中医药学刊,2018,36(2):374-377.
[9] 申震,朱付平,刘天举,等.桃红四物液对肢体缺血-再灌注损伤大鼠骨骼肌细胞凋亡的影响研究[J].辽宁中医杂志,2018,45(3):631-633.
[10] 申震,朱付平,潘成熙,等.SD大鼠肢体缺血-再灌注损伤模型的建立[J].中国比较医学杂志,2018,28(11):100-105.
[11] 沈甜,王斌,李敏,等.栀子苷与薯蓣皂苷对脑缺血再灌注大鼠急性期和恢复早期炎性损伤机制研究[J].陕西中医,2017,38(4):531-533.
[12] 陈欢,任越,贾万龙,等.丹参多酚酸对大鼠脑缺血再灌注后炎症因子的影响[J].陕西中医,2015,36(12):1690-1691.
[13] 闫磊,胡江平,邵新然,等.淫羊藿苷联合冰片对脑缺血-再灌注损伤模型大鼠抗氧化活性、炎症反应和血脑屏障通透性影响[J].陕西中医,2018,39(12):1659-1662.
[14] 曹美静,闫晨,郝丽娜.蒲参胶囊对大鼠心肌缺血再灌注损伤的保护作用[J].陕西医学杂志,2018,47(7):819-822.
[15] 苏权,党晓谦,王坤正,等.大鼠脊髓缺血再灌注后早期脊髓内促炎因子及抗炎因子的表达[J].陕西医学杂志,2016,45(10):1285-1287,1315.
[16] 唐天豪,张莹.NLRP3炎症小体在缺血再灌注损伤中的作用研究进展[J].现代消化及介入诊疗,2021,26(8):1052-1055,1059.
[17] 李运辉.HMGB1及TLR4-MyD88-NF-κB信号通路在急性肢体缺血再灌注损伤的作用及机制研究[D].济南:山东大学,2018.
[18] 米磊.HMGB1/RAGE促炎信号轴在下肢缺血再灌注血管内皮细胞损伤中的作用研究[D].济南:山东大学,2020.
[19] 代震,朱昱,王治阳.当归多糖对脑缺血再灌注损伤大鼠 IL-1β、TNF-α、NF-κB 表达的影响[J].中成药,2020,42(8):2176-2178.
[20] 崔昌胜,庄宝祥,吴洪娟,等.脉络宁注射液对兔肢体缺血再灌注损伤TNF-α和NF-κB的影响[J].中国药理学通报,2019,35(2):295-296.
[21] ZHU T,YAO Q,WANG W,et al.iNOS induces vascular endothelial cellmigration and apoptosis via autophagy in ischemia-reperfusion injury[J].Cellular Physiology and Biochemistry,2016,38(4):1575-1588.
[22] STEYERS C M,MILLER F J.Endothelial dysfunction in chronic inflammatory diseases[J].International J of Molecular Sciences,2014,15(7):11324-11349.
[23] GONG G,XIANG L,YUAN L,et al.Protective effect of glycyrrhizina direct HMGB 1 inhibitoron focal cerebral ischemia-reperfusion-induced inflammation oxidative stress and apoptosis in rats[J].PLoS One,2014,9(3):1237-1239.
[24] TONG Y,TANG Z,YANG T,et al.Ulinastatin preconditioning attenuates inflammatory reaction of hepatic ischemia reperfusion injury in rats via high mobility group box 1 inhibition[J].International J of Medical Sciences,2014,11(4):337.
[25] 高渐联,李汉伟,邓智建,等.泽兰甲醇提取物对慢性软组织损伤模型大鼠的影响及其机制[J].中成药,2018,40(3):537-543.
[26] 赵敏,刁丽,王淑梅,等.红花黄色素氯化钠注射液联合外敷冰黄散对四肢闭合骨折患者术后肿胀疼痛、炎症反应及凝血功能的影响[J].现代中西医结合杂志,2021,30(10):1083-1086.
[27] 栾冰,袁蓉,信琪琪,等.化瘀通络中药单体对VEGF诱导的人脐静脉内皮细胞血管新生和炎症反应的影响[J].中国中药杂志,2022,47(3):737-744.

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备注/Memo

备注/Memo:
基金项目:云南省科学技术厅中医联合专项课题(202101AZ070001-257,202101AZ070001-123); 云南省科学技术厅基础研究专项课题(202201AU070120); 昆明市卫生科技人才十百千项目[2021-SW(后备)-52]
更新日期/Last Update: 2024-11-11