[1]羊 羡,席建元,谢汶芳,等.银屑平丸调节cGAS-STING信号通路对银屑病小鼠的保护作用[J].陕西中医,2024,(11):1466-1470.[doi:DOI:10.3969/j.issn.1000-7369.2024.11.005]
　YANG Xian,XI Jianyuan,XIE Wenfang,et al.Protective effect of Yinxieping pill on psoriasis mice by regulating cGAS-STING signaling pathway[J].,2024,(11):1466-1470.[doi:DOI:10.3969/j.issn.1000-7369.2024.11.005]
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银屑平丸调节cGAS-STING信号通路对银屑病小鼠的保护作用
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年11期
页码:
1466-1470
栏目:
基础研究
出版日期:
2024-11-05

文章信息/Info

Title:
Protective effect of Yinxieping pill on psoriasis mice by regulating cGAS-STING signaling pathway
作者:
羊 羡; 席建元; 谢汶芳; 蒋宁兰; 彭礼真; 张元瑜; LIM Jian(新加坡); 祁 林
(湖南中医药大学第一附属医院,湖南 长沙 410007)
Author(s):
YANG Xian; XI Jianyuan; XIE Wenfang; JIANG Ninglan; PENG Lizhen; ZHANG Yuanyu; LIM Jian; QI Lin
(The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)
关键词:
银屑病;  银屑平丸;  GMP-AMP合成酶-干扰素基因刺激因子;  咪喹莫特;  免疫炎症;  皮肤损伤
Keywords:
Psoriasis;  Yinxieping pill;  GMP-AMP synthase-stimulator of interferon gene;  Imiquimod;  Immune inflammation;  Skin damage
分类号:
R 758.63
DOI:
DOI:10.3969/j.issn.1000-7369.2024.11.005
文献标志码:
A
摘要:
目的:探讨银屑平丸调节GMP-AMP合成酶(cGAS)-干扰素基因刺激因子(STING)信号通路对银屑病小鼠的保护作用。方法:取C57BL/6小鼠以咪喹莫特(IMQ)诱导银屑病模型,随机分为模型组、银屑平丸组、联合组,每组10只,另取10只C57BL/6小鼠作为对照组。用银屑平丸和RocA分组干预小鼠,检测其银屑病症状。HE染色检测小鼠皮肤组织病理变化; 免疫组化染色检测各组小鼠皮肤组织中性粒细胞浸润情况; 检测小鼠血清促炎因子与抗炎因子水平; 免疫印迹法检测小鼠皮肤组织cGAS-STING通路蛋白表达。结果:与对照组相比,模型组小鼠皮肤组织发生明显病理损伤,银屑病皮损面积及严重程度指数(PASI)、抓痒次数、皮肤表皮层厚度、髓过氧化物酶(MPO)阳性表达,IL-1β、IL-6、IL-17、IL-23及cGAS与STING蛋白表达升高,IL-4、TGF-β、IL-10水平降低,差异有统计学意义(均P<0.05)。与模型组比较,银屑平丸组小鼠皮肤组织病理损伤减轻,PASI、抓痒次数、皮肤表皮层厚度、MPO阳性表达、IL-1β、IL-6、IL-17、IL-23水平及cGAS与STING蛋白表达降低,IL-4、TGF-β及IL-10升高,差异有统计学意义(均P<0.05)。楝酰胺可减弱银屑平丸对银屑病小鼠的作用。结论:银屑平丸通过阻止cGAS-STING信号通路激活传导而减轻银屑病小鼠免疫炎症,改善其皮肤组织损伤症状,发挥保护作用。
Abstract:
Objective:To explore the protective effect of Yinxieping pill on psoriasis mice by regulating cyclic GMP-AMP synthase(cGAS)-stimulator of interferon gene(STING)signaling pathway.Methods:C57BL/6 mice were selected to induce psoriasis model with imiquimod(IMQ),and randomly separated into model group,Yinxieping pill group,combination group,10 mice in each group.Additional 10 C57BL/6 mice were included as control group.After intervention with Yinxieping pill and RocA of mice in each group,symptoms of psoriasis were detected.HE staining was applied to detect pathological changes in mouse skin tissue.Immunohistochemical staining was applied to detect infiltration of neutrophils in skin tissue of mice in each group.Levels of pro-inflammatory and anti-inflammatory factors in mouse serum were detected.Immunoblotting was applied to detect expression of cGAS-STING pathway proteins in mouse skin tissue.Results:Compared with the control group,the skin tissue of mice in the model group showed obvious pathological damage,PASI,number of scratching,thickness of the epidermis of the skin,myeloperoxidase positive expression,IL-1β,IL-6,IL-17 and IL-23 levels,cGAS and STING protein expression were obviously increased,and IL-4,TGF-β and IL-10 levels were obviously reduced,difference statistically significant(all P<0.05).Compared with the model group,the pathological damage of the skin tissue of mice in the Yinxieping pill group was reduced,PASI,number of scratching,thickness of the epidermis of the skin,myeloperoxidase positive expression,IL-1β,IL-6,IL-17 and IL-23 levels,cGAS and STING protein expression were reduced,and IL-4,TGF-β and IL-10 levels were increased,difference statistically significant(all P<0.05).Rocaglamide A can weaken the effect of Yinxieping pill on psoriasis mice.Conclusion:Yinxieping pill can alleviate immune inflammation in psoriasis mice by blocking activation and transmission of cGAS-STING signaling pathway,thereby improving their skin tissue damage symptoms and exerting a protective effect.

参考文献/References:

[1] DAN D,SRIVASTAVA N.Psoriasis:Striving for potential biomarkers[J].Assay Drug Dev Technol,2023,21(6):235-257.
[2] COSCARELLA G,MALVASO D,MANNINO M,et al.The preclinical discovery and development of deucravacitinib for the treatment of psoriasis[J].Expert Opin Drug Discov,2023,18(11):1201-1208.
[3] GUO J,ZHANG H,LIN W,et al.Signaling pathways and targeted therapies for psoriasis[J].Signal Transduct Target Ther,2023,8(1):437-474.
[4] 赵雨晴,魏志平,周迪,等.舒肝健脾养血法对寻常型银屑病患者免疫功能、炎症因子的影响[J].陕西中医,2022,43(1):69-72.
[5] MA J,SUN X,WU Z,et al.Correlation between the systemic immune inflammation index and risk of psoriasis:Results from NHANES[J].Eur J Dermatol,2024,34(1):31-39.
[6] ZHU Q,ZHAO L,DING H,et al.Interleukins and psoriasis[J].J Cutan Med Surg,2024,28(2):19-35.
[7] WILLEMSEN J,NEUHOFF M T,HOYLER T,et al.TNF leads to mtDNA release and cGAS/STING dependent interferon responses that support inflammatory arthritis[J].Cell Rep,2021,37(6):109977-109998.
[8] SONG M J,PARK C H,KIM H,et al.Carnitine acetyltransferase deficiency mediates mitochondrial dysfunction-induced cellular senescence in dermal fibroblasts[J].Aging Cell,2023,22(11):e14000-e14016.
[9] LAI Y,YANG N,CHEN X,et al.Dihydrocapsaicin suppresses the STING-mediated accumulation of ROS and NLRP3 inflammasome and alleviates apoptosis after ischemia-reperfusion injury of perforator skin flap[J].Phytother Res,2024,38(5):2539-2559.
[10] ZHANG Z,ZHOU D,LI Z,et al.A nanoinhibitor targeting cGAS-STING pathway to reverse the homeostatic imbalance of inflammation in psoriasis[J].Angew Chem Int Ed Engl,2024,63(2):e16007.
[11] 鲍秋羽,席建元.银屑平丸联合阿维A胶囊对银屑病样小鼠模型的疗效及IL-17、IL-23的影响[J].亚太传统医药,2022,18(4):26-30.
[12] 鲍秋羽,席建元,刘梨.基于Notch信号通路探讨银屑平丸对银屑病模型小鼠皮损及血清炎性因子的调节作用[J].陕西中医,2021,42(8):999-1004.
[13] 张喻,向雪川,周正繁,等.龙珠软膏对银屑病模型小鼠治疗作用的研究[J].中国医院药学杂志,2023,43(8):897-902,909.
[14] 张士伟,程慎令,邢启峰.茯苓酸通过抑制cGAS-STING信号通路减轻溃疡性结肠炎小鼠结肠上皮细胞损伤[J].免疫学杂志,2023,39(8):672-680.
[15] 杨梅,唐彩红,桂雨晴,等.咪喹莫特诱导银屑病模型小鼠T细胞免疫功能改变与性别的关系[J].中国药理学通报,2022,38(4):589-597.
[16] MORTLOCK R D,MA E C,COHEN J M,et al.Assessment of treatment-relevant immune biomarkers in psoriasis and atopic dermatitis:Toward personalized medicine in dermatology[J].J Invest Dermatol,2023,143(8):1412-1422.
[17] 刘俊峰,陈达灿,莫秀梅,等.陈达灿教授治疗寻常型银屑病经验[J].陕西中医,2022,43(8):1099-1101,1105.
[18] 李小鹏,席建元,谢汶芳,等.银屑平丸对寻常型银屑病患者外周血Th17相关细胞因子表达的影响[J].中国中西医结合皮肤性病学杂志,2019,18(6):558-561.
[19] 孙丹,闫小宁,耿蒙利,等.银屑平片对咪喹莫特诱导银屑病样模型小鼠IL-17、IL-23、PPARγ表达的影响[J].中国中医药信息杂志,2022,29(4):64-68.
[20] YANG S F,LIN M H,CHOU P C,et al.Genetics of generalized pustular psoriasis:Current understanding and implications for future therapeutics[J].Genes,2023,14(6):1297-1308.
[21] MATSUNO A,SUMIDA H,NAKANISHI H,et al.Keratinocyte proline-rich protein modulates immune and epidermal response in imiquimod-induced psoriatic skin inflammation[J].Exp Dermatol,2023,32(12):2121-2130.
[22] THAPAR M,PATEL M,GORDON K.Bimekizumab for the treatment of psoriasis[J].Immunotherapy,2024,16(7):431-446.
[23] HUANG C,LI W,REN X,et al.The crucial roles and research advances of cGAS-STING pathway in cutaneous disorders[J].Inflammation,2023,46(4):1161-1176.
[24] LUAN C,HE Y,LIU W,et al.PCSK9 inhibition interrupts the cross-talk between keratinocytes and macrophages and prevents UVB-induced skin damage[J].J Biol Chem,2023,299(7):104895-104906.

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备注/Memo

备注/Memo:
基金项目:湖南省自然科学基金资助面上项目(2023JJ30474); 湖南省卫生健康委员会科研计划项目(D202304127949); 湖南省中药管理局中医药科研计划项目(B2023031)
更新日期/Last Update: 2024-11-11