[1]李慎富,朱付平,黄泓珺.桃红四物汤通过miR-675/NF-κB信号通路减轻肢体缺血再灌注损伤炎症反应机制研究[J].陕西中医,2024,(12):1592-1597.[doi:DOI:10.3969/j.issn.1000-7369.2024.12.002]
　LI Shenfu,ZHU Fuping,HUANG Hongjun.The mechanism of Taohong Siwu decoction reducing inflammation in limb ischemia-reperfusion injury through miR-675/NF-κB signaling pathway[J].,2024,(12):1592-1597.[doi:DOI:10.3969/j.issn.1000-7369.2024.12.002]
点击复制

桃红四物汤通过miR-675/NF-κB信号通路减轻肢体缺血再灌注损伤炎症反应机制研究
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年12期
页码:
1592-1597
栏目:
基础研究
出版日期:
2024-12-05

文章信息/Info

Title:
The mechanism of Taohong Siwu decoction reducing inflammation in limb ischemia-reperfusion injury through miR-675/NF-κB signaling pathway
作者:
李慎富1; 朱付平2; 黄泓珺1
(1.湖南中医药大学,湖南 长沙 410208; 2.湖南中医药大学第一附属医院,湖南 长沙 410021)
Author(s):
LI Shenfu; ZHU Fuping; HUANG Hongjun
(Hunan University of Traditional Chinese Medicine,Changsha 410208,China)
关键词:
肢体缺血再灌注损伤;  miR-675-5p;  核转录因子κB;  桃红四物汤;  炎症因子;  信号通路
Keywords:
Limb ischemia-reperfusion injury;  miR-675-5p;  NF-κB;  Taohong Siwu decoction;  Inflammatory factor;  Signaling pathway
分类号:
R 285.5
DOI:
DOI:10.3969/j.issn.1000-7369.2024.12.002
文献标志码:
A
摘要:
目的:基于miR-675/NF-κB信号通路研究桃红四物汤对肢体缺血再灌注损伤炎症反应的影响。方法:78只大鼠分为四组,除正常对照组外,其余三组均复制缺血再灌注损伤模型。于再灌注0、2、4、8 h四个时间点取材骨骼肌,HE染色观察骨骼肌形态; TUNEL荧光染色观察骨骼肌细胞凋亡情况; RT-qPCR检测miR-675-5p、NF-κB p65、p50表达; Western blot检测NF-κB p65、p50蛋白表达; ELISA检测血清TNF-α、IL-1β、IL-6水平。结果:与模型组相比,桃红四物汤组、miR-675阻滞剂组血清TNF-α、IL-1β、IL-6水平降低(均P<0.05); 桃红四物汤、miR-675阻滞剂干预,可保护骨骼肌细胞,减轻凋亡程度,与模型组相比,可降低NF-κB p65、p50的表达(均P<0.05)。结论:桃红四物汤可通过减轻炎症反应保护骨骼肌细胞,这一过程可能与miR-675/NF-κB信号通路的激活有关。
Abstract:
Objective:To study the effect of Taohong Siwu decoction on inflammatory response of limb ischemia-reperfusion injury based on miR-675/NF-κB signaling pathway.Methods:Seventy-eight rats were divided into four groups.Except for the normal control group,the other three groups were replicated for ischemia-reperfusion injury,and then skeletal muscle was taken at four time points of 0,2,4 and 8 hours.HE staining was used to observe the morphology of skeletal muscle.The apoptosis of skeletal muscle cells was observed by TUNEL fluorescence staining.RT-qPCR was used to detect the expression of miR-675-5p and NF-κB p65,p50 in cells.The expression of NF-κB p65,p50 in cells was detected by Western blot.The concentrations of TNF-α,IL-1β and IL-6 in serum were quantified by ELISA.Results:Compared with the model group,the levels of serum TNF-α,IL-1β and IL-6 in the Taohong Siwu decoction and miR-675 inhibitor groups were significantly lower(all P<0.05).Under the intervention of Taohong Siwu decoction and miR-675 inhibitor,it can protect skeletal muscle cells and reduce the degree of apoptosis.Compared with the model group,it can also reduce the expression of NF-κB p65,p50(all P<0.05).Conclusion:Taohong Siwu decoction can protect skeletal muscle cells by reducing inflammatory response,which may be related to the activation of miR-675/NF-κB signaling pathway.

参考文献/References:

[1] 施嘉华,陈真珍,黄龙坚,等.铁死亡在脑缺血再灌注中的作用研究进展[J].陕西医学杂志,2024,53(1):132-135.
[2] WU M Y,YIANG G T,LIAO W T,et al.Current mechanistic concepts in ischemia and reperfusion injury[J].Cel Physiol and Biochem,2018,46(4):1650-1667.
[3] 谷岩,何菊,侯骊坤,等.急性下肢缺血再灌注损伤机制的研究进展[J].齐齐哈尔医学院学报,2020,41(12):1521-1522.
[4] RASHEED Z,AKHTAR N,HAQQI T M.Advanced glycation end products induce the expression of interleukin-6 and interleukin-8 by receptor for advanced glycation end product-mediated activation of mitogen-activated protein kinases and nuclear factor-κB in human osteoarthritis chondrocytes[J].Rheumatology,2011,50(5):838-851.
[5] 申震,朱付平,黄勇,等.Wnt/Ca2+信号通路在肢体缺血/再灌注损伤机制中的研究进展[J].中国药理学通报,2018,34(10):1344-1347.
[6] WANG B,SUEN C W,MA H,et al.The roles of H19 in regulating inflammation and aging[J].Front Immunol,2020,11:579687.
[7] 张航.Hsa-miR-675-5p在食管鳞癌中的表达及其功能机制研究[D].长沙:中南大学,2013.
[8] SUN T,LEUNG F,LU W W.miR-9-5p,miR-675-5p and miR-138-5p damages the strontium and LRP5-mediated skeletal cell proliferation,differentiation,and adhesion[J].Int J Mol Sci,2016,17(2):236.
[9] 刘宗义,朱付平,李武平.桃红四物汤对大鼠肢体缺血-再灌注损伤骨骼肌长链非编码RNA H19、核因子κB-p65表达的影响[J].陕西中医,2022,43(9):1163-1169.
[10] ZHU F P,LI W P,WANG L H,et al.Tao-Hong-Si-Wu decoction reduces ischemia reperfusion rat myoblast cells calcium overloading and inflammation through the Wnt/IP3R/CAMKⅡ pathway[J].J Cell Biochem,2019,120(8):13095-13106.
[11] 申震,朱付平,潘成熙,等.SD大鼠肢体缺血-再灌注损伤模型的建立[J].中国比较医学杂志,2018,28(11):100-105.
[12] 王银庄,张继东,刘磊.桃红四物汤化裁治疗稳定型心绞痛合并心力衰竭临床研究[J].陕西中医,2020,41(5):625-628.
[13] 陈梦,石丹宁,张则业,等.四物汤的药理作用与临床应用研究进展[J].中国医药,2021,16(12):1917-1920.
[14] JIANG H,LI M,DU K,et al.Traditional Chinese medicine for adjuvant treatment of breast cancer:Taohong Siwu decoction[J].Chin Med,2021,16:1-20.
[15] 储霞,董怡,苗芙.桃红四物汤在临床应用中的研究进展[J].当代护士(下旬刊),2016,24(5):3-4.
[16] YAO J.Acupuncture theory of promoting blood circulation and removing stasis and its clinical application[J].Zhongguo Zhenjiu,2015,35(4):389-392.
[17] SHAO C L,CUI G H,GUO H D.Effects and mechanisms of Taohong Siwu decoction on the prevention and treatment of myocardial injury[J].Front in Pharmacol,2022,13:816347.
[18] LU X,LI J,ZHOU B,et al.Taohong Siwu decoction enhances human bone marrow mesenchymal stem cells proliferation,migration and osteogenic differentiation via VEGF-FAK signaling in vitro[J].J Ethnopharmacol,2023,307:116203.
[19] WANG J X,ZHANG X J,LI Q,et al.MicroRNA-103/107 regulate programmed necrosis and myocardial ischemia/reperfusion injury through targeting FADD[J].Circ Res,2015,117(4):352-363.
[20] PAN J X.LncRNA H19 promotes atherosclerosis by regulating MAPK and NF-κB signaling pathway[J].Eur Rev Med Pharmacol Sci,2017,21(2):322-328.
[21] WANG H,SHI M J,HU Z Q,et al.Effect of miR-206 on lower limb ischemia-reperfusion injury in rat and its mechanism[J].Sci Rep,2023,13(1):21574.
[22] LIU L,WANG F E,FENG Q,et al.Mechanism of MIR-210 mediated NF-κB pathway on cardiac ischemia-reperfusion injury[J].J Biol Regul Homeost Agents,2021,35:1-7.
[23] ZHANG W,HUANG Z,XIAO Z,et al.NF-κB downstream miR-1262 disturbs colon cancer cell malignant behaviors by targeting FGFR1[J].Acta Biochim Biophys Sin,2023,55(11):1819-1832.
[24] LIAN J,ZHU X,DU J,et al.Extracellular vesicle-transmitted miR-671-5p alleviates lung inflammation and injury by regulating the AAK1/NF-κB axis[J].Mol Ther,2023,31(5):1365-1382.
[25] 袁浩鑫,冯一平,王思雨,等.调控miR-642a-5p表达对宫颈癌HeLa细胞生物学行为的影响及机制研究[J].陕西医学杂志,2023,52(11):1498-1501,1525.

相似文献/References:

[1]邓文芸,黄雅静,申 震.泽兰合剂对肢体缺血再灌注损伤大鼠炎性损伤的作用[J].陕西中医,2024,(11):1460.[doi:DOI:10.3969/j.issn.1000-7369.2024.11.004]
　DENG Wenyun,HUANG Yajing,SHEN Zhen.Effects of Zelan mixture on inflammatory damage of rats with limb ischemia-reperfusion injury[J].,2024,(12):1460.[doi:DOI:10.3969/j.issn.1000-7369.2024.11.004]

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(82274541); 湖南省自然科学基金资助项目(2020JJ4070)
更新日期/Last Update: 2024-12-05