[1]廖飞飞,杨文文,路爱梅,等.麝香通心滴丸调控HIF-1α/HO-1通路抑制铁死亡缓解心肌缺血再灌注损伤研究[J].陕西中医,2025,46(2):147-152,158.[doi:DOI:10.3969/j.issn.1000-7369.2025.02.001]
　LIAO Feifei,YANG Wenwen,LU Aimei,et al.Shexiang Tongxin dripping pills alleviate myocardial ischemia-reperfusion injury through inhibition of ferroptosis via regulation of the HIF-1α/HO-1 signaling pathway[J].,2025,46(2):147-152,158.[doi:DOI:10.3969/j.issn.1000-7369.2025.02.001]
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麝香通心滴丸调控HIF-1α/HO-1通路抑制铁死亡缓解心肌缺血再灌注损伤研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年2期
页码:
147-152,158
栏目:
基础研究
出版日期:
2025-02-05

文章信息/Info

Title:
Shexiang Tongxin dripping pills alleviate myocardial ischemia-reperfusion injury through inhibition of ferroptosis via regulation of the HIF-1α/HO-1 signaling pathway
作者:
廖飞飞1; 2; 杨文文3; 路爱梅1; 4; 曲 华2; 王 洁2; 龙霖梓2; 付长庚2
(1.北京中医药大学研究生院,北京 100029; 2.中国中医科学院西苑医院,北京 100091; 3.陕西省中医医院心病科,陕西 西安 710003; 4.北京中医药大学东直门医院,北京 100007)
Author(s):
LIAO Feifei1; 2; YANG Wenwen3; LU Aimei1; 4; QU Hua2; WANG Jie 2; LONG Linzi2; FU Changgeng2
(1.Graduate School,Beijing University of Chinese Medicine,Beijing 100029,China; 2.Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China; 3.Department of Cardiology,Shaanxi Provincial Hospital of Traditional Chinese Medicine,Xi'an 710003,China; 4.Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100007,China)
关键词:
心肌缺血再灌注损伤;  缺氧/复氧;  麝香通心滴丸;  铁死亡;  HIF-1α/HO-1信号通路
Keywords:
Myocardial ischemia-reperfusion injury;  Hypoxia/reoxygenation;  Shexiang Tongxin dripping pills;  Ferroptosis;  HIF-1α/HO-1 signaling pathway
分类号:
R 542.22
DOI:
DOI:10.3969/j.issn.1000-7369.2025.02.001
文献标志码:
A
摘要:
目的:探究麝香通心滴丸(STDP)对心肌缺血再灌注损伤(MIRI)的保护作用及其潜在机制。方法:采用AnaeroPack方法构建H9c2细胞缺氧复氧损伤(H/R)模型以模拟MIRI。将细胞随机分为对照组、模型组和麝香通心滴丸组,分别在不含药或含药的培养液中培养24 h后收集细胞。采用细胞计数试剂盒法(CCK-8)检测细胞增殖与活性,酶联免疫吸附法(ELISA)测定细胞上清液乳酸脱氢酶(LDH)水平,荧光探针法检测活性氧(ROS)水平以评估细胞的氧化应激状态,透射电镜(TEM)观察线粒体超微结构变化,Western blot检测低氧诱导因子-1α(HIF-1α)、血红素加氧酶-1(HO-1)信号通路相关蛋白的表达水平。结果:麝香通心滴丸在H9c2心肌细胞中未表现出明显细胞毒性,同时在H/R条件下显著抑制了细胞损伤。麝香通心滴丸通过降低ROS生成和LDH释放,显著缓解了H/R诱导的氧化应激。TEM观察显示,与对照组相比,模型组的线粒体呈现明显的萎缩并伴随典型的铁死亡特征,而麝香通心滴丸组的线粒体形态显著改善,趋于正常。进一步通过Western blot检测发现,模型组HIF-1α和HO-1蛋白表达显著上调,而麝香通心滴丸组显著抑制了这些蛋白的过度表达(P<0.05)。结论:麝香通心滴丸可能通过抑制HIF-1α/HO-1信号通路介导的铁死亡,减轻心肌缺血再灌注损伤。
Abstract:
Objective:To investigate the protective effects of Shexiang Tongxin dripping pills(STDP)on myocardial ischemia-reperfusion injury(MIRI)and elucidate the underlying mechanisms.Methods:An H9c2 cell hypoxia-reoxygenation(H/R)injury model was established using the AnaeroPack method to simulate MIRI.Cells were randomly divided into control,model,and STDP groups and cultured in STDP-free or STDP-containing medium for 24 hours before being harvested.Cell proliferation and viability were assessed using the Cell Counting Kit-8(CCK-8)assay.Lactate dehydrogenase(LDH)levels in the cell supernatant were measured using enzyme-linked immunosorbent assay(ELISA).Reactive oxygen species(ROS)levels were evaluated using a fluorescence probe method to assess oxidative stress.Mitochondrial ultrastructural changes were observed by transmission electron microscopy(TEM).Protein expression levels of hypoxia-inducible factor-1α(HIF-1α)and heme oxygenase-1(HO-1)in the signaling pathway were analyzed by Western blot.Results:STDP-containing medium exhibited no significant cytotoxicity in H9c2 cardiomyocytes and effectively reduced H/R-induced cell injury.STDP alleviated oxidative stress by decreasing ROS generation and LDH release.TEM observations revealed that,compared with the control group,the model group showed significantly shrunken mitochondria with characteristic ferroptosis features,whereas mitochondrial morphology in the STDP group was markedly improved,resembling normal conditions.Furthermore,Western blot analysis demonstrated that the expression levels of HIF-1α and HO-1 proteins were significantly upregulatedin the model group,whereas STDP treatment significantly suppressed the excessive expression of these proteins(P<0.05).Conclusion:STDP may alleviate MIRI by inhibiting ferroptosis mediated via the HIF-1α/HO-1 signaling pathway.

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备注/Memo

备注/Memo:
[基金项目]国家自然科学基金资助项目(82374413); 中国中医科学院科技创新工程(CI2021A05013)
更新日期/Last Update: 2025-02-08