[1]李鹏,马文慧,周靖雅.西北燥证动物模型的优化与评价[J].陕西中医,2026,(4):435-440.[doi:DOI:10.3969/j.issn.1000-7369.2026.04.001]
　LI Peng,MA Wenhui,ZHOU Jingya.Optimization and evaluation of animal model of northwest dryness syndrome[J].,2026,(4):435-440.[doi:DOI:10.3969/j.issn.1000-7369.2026.04.001]
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西北燥证动物模型的优化与评价

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2026年4期
页码:
435-440
栏目:
基础研究
出版日期:
2026-04-05

文章信息/Info

Title:
Optimization and evaluation of animal model of northwest dryness syndrome
作者:
李鹏1; 马文慧2; 周靖雅1
（1.新疆医科大学附属中医医院，新疆 乌鲁木齐 830000；2.新疆医科大学第四临床医学院，新疆 乌鲁木齐 830099）
Author(s):
LI Peng1; MA Wenhui2; ZHOU Jingya1
（1.The Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University，Urumqi 830000，China；2.The Fourth Clinical Medical College of Xinjiang Medical University，Urumqi 830099，China）
关键词:
西北燥证; 动物模型; 大鼠; 人工气候沙尘箱; 造模方法; 评价指标
Keywords:
Northwest dryness syndrome; Animal model; Rat; Artificial climate and dust chamber; Modeling method; Evaluation index
分类号:
R 254
DOI:
DOI:10.3969/j.issn.1000-7369.2026.04.001
文献标志码:
A
摘要:
目的：在既往西北燥证造模方法基础上，依据西北燥证复杂病因特点，进一步优化西北燥证动物模型造模与评价方法，创建一体式人工气候箱，模拟西北燥证自然病因构成，以期优化后的西北燥证动物模型更加符合临床实际，更具有可复制性。方法：将10只SD雄性大鼠随机分为空白组5只和模型组5只，饲养4个西北燥证病因气化周期（24 d）。测定两组大鼠唾液分泌量、粪便含水率、颌下腺器官指数、泪液分泌量，统计大鼠舌质三原色（RGB）值，采用HE染色观察泪液病理形态。结果：与空白组比较，模型组大鼠舌尖R值显著降低(P＜0.01)，G值、B值显著升高(P＜0.05或P＜0.01)。相同病因气化周期，模型组大鼠体重增长量较空白组低，且差异有统计学意义（均P<0.05）。模型组大鼠唾液分泌量、粪便含水率、颌下腺器官指数、泪液分泌量等与空白组比较差异均有统计学意义（P<0.05或P<0.01)。泪液结晶病理分析：空白组大鼠泪液结晶黏附性好，呈现大片的多级分枝乳头状，似圣诞树；模型组大鼠结晶失黏附，呈现散在分布的大小不一的微乳头样结构，极向紊乱。结论：优化后的西北燥证动物模型行为学评分更为均一，动态视频监测动物症状评分更加准确可靠，优化后的西北燥证动物模型更具有可复制性，且更加符合临床实际，有利于西北燥证相关基础与实验研究。
Abstract:
Objective:Based on the existing modeling methods for northwest dryness syndrome (NDS) and in light of the complex pathogenic characteristics of NDS,this study aimed to further optimize the modeling and evaluation methods of the NDS animal model,develop an integrated artificial climate chamber,and simulate the natural pathogenic composition of NDS.The goal was to establish an optimized NDS animal model that is more consistent with clinical practice and possesses higher reproducibility.Methods:Ten male SD rats were randomly divided into a blank group (n=5) and a model group (n=5),and were fed for 4 etiological Qi-transformation cycles (24 d) of the northwest dryness syndrome.The general condition of rats was observed,and the amount of drinking water,food intake,body weight,saliva secretion,fecal water content,submandibular gland organ index,tear secretion,tongue trichrome (RGB) and tear pathology were measured.HE staining was used to observe the pathological morphology of tears.Results:Compared with the blank group,the R value of the tip of the tongue in the model group rats decreased significantly (P<0.01),while the G value and B value increased significantly (P<0.05 or P<0.01).During the same pathogenic qi-transformation cycle,the weight gain of the model group rats was lower than that of the blank group,and the difference was statistically significant (all P<0.05).There were statistically significant differences in the salivary secretion volume,fecal moisture content,submandibular gland organ index,and lacrimal secretion volume between the model group and the blank group (P<0.05 or P<0.01).In the blank group,the lacrimal crystallization of rats had good adhesion,showing large-scale multi-level branched papillae,resembling a Christmas tree.In the model group,the crystallization lost adhesion,presenting scattered micro-papillary structures of different sizes with disordered polarity.Conclusion:The optimized animal model of NDS exhibits more uniform behavioral scores and a lower mortality rate.Meanwhile,the symptom scores obtained via dynamic video monitoring are more accurate and reliable.In addition,this optimized model features higher reproducibility,greater consistency with clinical practice,and ease of popularization and application,which greatly facilitates the basic and experimental research related to NDS.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（82260917）；新疆维吾尔自治区自然科学基金重点资助项目(2022D01D49)
更新日期/Last Update: 2026-04-05