[1]胡方竹,吴日辉,谢丽,等.基于铁死亡代谢探讨电针心经对大鼠缺血性脑卒中的作用机制[J].陕西中医,2025,46(11):1469-1475.[doi:DOI:10.3969/j.issn.1000-7369.2025.11.005]
　HU Fangzhu,WU Rihui,XIE Li,et al.Mechanism of electroacupuncture at heart meridian points on the ferroptosis metabolic pathway in rats with ischemic stroke[J].,2025,46(11):1469-1475.[doi:DOI:10.3969/j.issn.1000-7369.2025.11.005]
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基于铁死亡代谢探讨电针心经对大鼠缺血性脑卒中的作用机制
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年11期
页码:
1469-1475
栏目:
基础研究
出版日期:
2025-11-05

文章信息/Info

Title:
Mechanism of electroacupuncture at heart meridian points on the ferroptosis metabolic pathway in rats with ischemic stroke
作者:
胡方竹; 吴日辉; 谢丽; 徐晓隽; 段传; 梁依琳; 潘江
（湖南中医药大学第一附属医院，湖南 长沙 410007）
Author(s):
HU Fangzhu; WU Rihui; XIE Li; XU Xiaojun; DUAN Chuan; LIANG Yilin; PAN Jiang
(The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)
关键词:
脑缺血; 铁代谢; 铁死亡; 心经穴; 电针
Keywords:
Cerebral infarction; Iron metabolism disorders; Ferroptosis; Heart meridian acupoints; Electroacupuncture
分类号:
R 255.2
DOI:
DOI:10.3969/j.issn.1000-7369.2025.11.005
文献标志码:
A
摘要:
目的：探讨电针心经穴对缺血性脑卒中大鼠神经元铁死亡铁代谢途径相关蛋白表达的影响。方法：雄性SD大鼠随机分成假手术组、模型组、心经组、非穴组，第1、7天两个时间点开展观察。采用改良线栓法制备大鼠模型，心经组电针患肢心经“极泉”“少海”“灵道”“神门”，非穴组电针患肢同水平节段非穴点，每次30 min/d。采用Longa评分评估神经功能损伤程度；TTC染色测脑梗死体积；HE染色观察脑组织形态结构；尼氏染色观察缺血半暗带神经元数量；ELISA检测缺血区细胞亚铁离子（Fe2）、丙二醛（MDA）含量；Western Blot检测缺血半暗带核因子E2相关因子2（Nrf2）、铁蛋白重链1（FTH1）、铁蛋白轻链（FTL）、铁转运蛋白1（FPN1）的蛋白表达含量。结果：与假手术组比较，模型组神经功能缺损评分、脑梗死体积、Fe2、MDA含量升高，神经元数量及Nrf2、FTH1、FTL、FPN1蛋白表达降低（均P＜0.05）。与模型组比较，心经组神经功能改善、脑梗死体积缩小，神经元数量增加，组织细胞Fe2、MDA含量降低，Nrf2、FTH1、FTL、FPN1蛋白表达增加（均P＜0.05）。非穴组各方面比较，差异无统计学意义（P＞0.05）。心经组第1、7天比较，神经功能、脑梗死体积下降（均P＜0.05）。Nrf2、FTH1、FTL、FPN1蛋白表达均增加（均P＜0.05）。结论：电针心经穴可减轻MCAO大鼠神经损伤，其机制可能与激活Nrf2信号通路、调控铁代谢相关蛋白、抑制神经元铁死亡有关。
Abstract:
Objective:To investigate effect of electroacupuncture at heart meridian points on the expression of proteins related to the ferroptosis iron metabolism pathway in neurons of rats with ischemic stroke.Methods:Male sprague-dawley (SD) rats were randomly divided into sham group,model group,Heart Meridian group,and non-acupoint group,with two time points first and seven days.The model was established using the modified longa filament method.The heart meridian group received EA at Jiquan Shaohai Lingdao and Shenmen on the affected limb,while the non-acupoint group received EA at non-acupoint points at the same segment level on the affected limb.Each session lasted 30 minutes daily.Neurological deficits were assessed using Longa scores;cerebral infarction volume was measured via TTC staining;histopathological changes were observed by HE staining;neuronal survival was evaluated via Nissl staining;Fe2 content and MDA in ischemic tissues was quantified by ELISA;and protein expression levels of nuclear factor erythroid 2-related factor 2 FTH,FTL and ferroportin 1 were analyzed by Western Blot.Results:Compared with the sham group,the model group showed increased neurological deficit scores,larger infarct volume,elevated Fe2 and MDA levels (P<0.05),and decreased neuronal count and protein expression of Nrf2,FTH1,FTL,and FPN1 (P<0.05).Compared with the model group,the heart meridian group exhibited improved neurological function,reduced infarct volume,increased neuronal count,decreased Fe2 and MDA levels (P<0.05),and upregulation of Nrf2,FTH1,FTL,and FPN1 protein expression (P<0.05).The non-acupoint group showed no significant differences compared to the model group (P>0.05).Within the heart meridian group,comparison between the first and seven days showed further improvement in neurological function and infarct volume (P<0.05),along with increased expression of Nrf2,FTH1,FTL,and FPN1 proteins (P<0.05).Conclusion:Electroacupuntcture at heart meridian points can alleviate neurological impairment in rats,possibly through activating the Nrf2 pathway,regulating iron metabolism-related proteins,and inhibiting neuronal ferroptosis.

参考文献/References:

[1]FEIGIN V L,BRAININ M,NORRVING B,et al.World stroke organization:Global stroke fact sheet 2025[J].International Journal of Stroke:Official Journal of the International Stroke Society,2025,20：132-144.
[2]MA Q,LI R,WANG L,et al.Temporal trend and attributable risk factors of stroke burden in China 1990-2019:An analysis for the global burden of disease study 2019[J].The Lancet Public Health,2021,6：e897-e906.
[3]中国卒中中心联盟.《中国卒中中心报告2022》概要[J].中国脑血管病杂志,2024,21(8):565-576.
[4]SJ D,KM L,MR L,et al.Ferroptosis:An iron-dependent form of nonapoptotic cell death[J].Cell,2012,149(5)：1060-1072.
[5]JIANG X,STOCKWELL B R,CONRAD M.Ferroptosis:Mechanisms biology and role in disease[J].Nature Reviews Molecular Cell Biology,2021,22(4):266-282.
[6]CHEN J,YANG L,GENG L,et al.Inhibition of acyl-CoA synthetase long-chain family member 4 facilitates neurological recovery after stroke by regulation ferroptosis[J].Frontiers in Cellular Neuroscience,2021,15：632354.
[7]LUO Q,ZHENG J,FAN B,et al.Enriched environment attenuates ferroptosis after cerebral Ischemia/Reperfusion injury by regulating iron metabolism[J].Brain Research Bulletin,2023,203:110778.
[8]ZHAN S,LIANG J,LIN H,et al.SATB1/SLC7A11/HO-1 axis ameliorates ferroptosis in neuron cells after ischemic stroke by danhong injection[J].Molecular Neurobiology,2023,60:413-427.
[9]YANG L,ZHANG M,LIU M,et al.Loss of FTH1 induces ferritinophagy-mediated ferroptosis in anaemia of myelodysplastic syndromes[J].Journal of Cellular and Molecular Medicine,2025,29:e70350.
[10]WANG L,ZHANG X,XIONG X,et al.Nrf2 regulates oxidative stress and its role in cerebral ischemic stroke[J].Antioxidants,2022,11(1):2377.
[11]刘然,张宏.针刺治疗中风病选穴配穴思路和特色针法研究进展[J].四川中医,2021,39(6):216-218.
[12]韩佳悦,潘单依,肖佳璇,等.从“瘀毒”病机探讨脑缺血再灌注铁死亡机制及活血化瘀解毒方干预机制[J].中国实验方剂学杂志,2024，31（8）:51-60.
[13]陈成,章薇,娄必丹,等.电针心包经穴对急性脑缺血大鼠血清及脑组织神经生长因子和神经生长抑制因子的影响[J].针刺研究,2015,40(2):94-98.
[14]石文英,严洁,常小荣,等.基于心脑相关理论探讨电针心经、心包经穴对大脑中动脉梗阻大鼠心、脑细胞凋亡的影响[J].针刺研究,2019,44(2):107-112.
[15]潘江,曹洋,陈成,等.电针心经/心包经穴对MCAO/R大鼠MeCP2磷酸化影响的研究[J].湖南中医药大学学报,2022,42(11):1884-1890.
[16]DEHQANIZADEH B,MOHAMMADI Z F,KALANI A H T,et al.Effect of early exercise on inflammatory parameters and apoptosis in CA1 area of the hippocampus following cerebral ischemia-reperfusion in rats[J].Brain Research Bulletin,2022,182:102-110.
[17]EZ L,PR W,SC C,et al.Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke,1989,20（1）：84-91.
[18]潘江,章薇,葛卫军,等.改良永久性线栓法制作大鼠大脑中动脉梗阻模型的探讨[J].湖南中医药大学学报,2011,31(3):2624-2644.
[19]中国针灸学会.实验动物常用穴位名称与定位第2部分：大鼠[J].针刺研究,2021,46(4):351-352.
[20]TAO J,ZHOU J,ZHU H,et al.Tetramethylpyrazine inhibits ferroptosis in spinal cord injury by regulating iron metabolism through the NRF2/ARE pathway[J].Frontiers in Pharmacology,2024,15:1503064.
[21]YAN R,LIN B,JIN W,et al.NRF2 a superstar of ferroptosis[J].Antioxidants,2023,12(9):1739.
[22]DUSEK P,HOFER T,ALEXANDER J,et al.Cerebral iron deposition in neurodegeneration[J].Biomolecules,2022,12(5):714.
[23]TIAN H,XIONG Y,ZHANG Y,et al.Activation of NRF2/FPN1 pathway attenuates myocardial ischemia-reperfusion injury in diabetic rats by regulating iron homeostasis and ferroptosis[J].Cell Stress & Chaperones,2022,27:149-164.
[24]WANG H J,TANG H,JIANG S S,et al.Effects of acupuncture on iron metabolism in the hippocampus of the rats with cerebral ischemia-reperfusion injury based on the imbalance of iron homeostasis[J].World Journal of Acupuncture-Moxibustion,2024,34(4):292-299.
[25]YANG X，CHEN,JIN Y J,et al.Electroacupuncture attenuates ferroptosis by promoting Nrf2 nuclear translocation and activating Nrf2/SLC7A11/GPX4 pathway in ischemic stroke[J].Chinese Medicine,2025,20（1）:4.
[26]王琪,刘瑶瑶,侯子文.“醒脑开窍”针刺法对脑缺血再灌注损伤大鼠铁死亡相关因子表达的影响[J].针刺研究,2024,49(10):1019-1029.
[27]蒋思怡,陶锋,孟智宏.针刺干预小胶质细胞铁死亡治疗缺血性脑卒中研究进展[J].陕西中医,2025,46(8):1146-1149.
[28]冯恩敏,凌志强,左世国,等.基于时间点探讨针刺对脑缺血再灌注损伤大鼠脑组织VEGF表达的影响[J].中国中医药现代远程教育,2020,18(20):116-119.
[29]卢小叶,郑慧娥,吕倩忆,等.基于不同时间点观察针刺对脑缺血再灌注损伤大鼠海马组织Beclin-1及Caspase-3表达的影响（英文）[J].World Journal of Acupuncture-moxibustion,2023,33(3):228-236.

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备注/Memo

备注/Memo:
国家自然科学青年基金资助项目（82105002）
更新日期/Last Update: 2025-11-04