[1]覃颖,李笑涵,吴江滨,等.孙氏头腹针刺法对焦虑性失眠大鼠BDNF-TrkB信号通路的影响[J].陕西中医,2025,46(11):1476-1480,1519.[doi:DOI:10.3969/j.issn.1000-7369.2025.11.006]
　QIN Ying,LI Xiaohan,WU Jiangbin,et al.Mechanism of Sun’s head-abdominal acupuncture on anxiety-induced insomnia rats via the BDNF-TrkB signaling pathway[J].,2025,46(11):1476-1480,1519.[doi:DOI:10.3969/j.issn.1000-7369.2025.11.006]
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孙氏头腹针刺法对焦虑性失眠大鼠BDNF-TrkB信号通路的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年11期
页码:
1476-1480,1519
栏目:
基础研究
出版日期:
2025-11-05

文章信息/Info

Title:
Mechanism of Sun’s head-abdominal acupuncture on anxiety-induced insomnia rats via the BDNF-TrkB signaling pathway
作者:
覃颖1; 李笑涵1; 吴江滨2; 秦丽娜1
（1.北京中医药大学第三附属医院康复科，北京 100029；2.德国魁茨汀中医院，德国 汉堡999035）
Author(s):
QIN Ying1; LI Xiaohan1; WU Jiangbin2; QIN Lina1
（1.Department of Rehabilitation,The Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China；2.German Kuiciting Traditional Chinese Medicine Hospital，Hamburg 999035，Germany）
关键词:
焦虑性失眠; 孙氏头腹针; 脑源性神经营养因子; 酪氨酸激酶受体 B; 戊巴比妥钠协同睡眠实验
Keywords:
Anxiety insomnia; Sun’s head-abdominal acupuncture; BDNF; TrkB; Pentobarbital-induced sleep test
分类号:
R 256.23
DOI:
DOI:10.3969/j.issn.1000-7369.2025.11.006
文献标志码:
A
摘要:
目的：观察孙氏头腹针对焦虑性失眠大鼠BDNF-TrkB信号通路表达的影响。方法：将SD大鼠随机分为空白组、模型组、常规针刺组、头腹针刺组。除空白组外，其他三组采用睡眠剥夺、焦虑型心理应激法结合腹腔注射对氯苯丙氨酸法联合复制焦虑性失眠大鼠模型。常规针刺组进行常规穴位针刺治疗，头腹针刺组给予头针与腹针联合治疗，头腹针刺组在头部穴位进行电针干预，频率为2 Hz连续波，电流约0.5 mA，每日1次，持续时间30 min，连续干预1周。比较各组大鼠的戊巴比妥钠协同睡眠实验、旷场试验（OFT）等行为学指标。采用蛋白质印迹法（Western blotting,WB）与实时荧光定量聚合酶链式反应（Quantitative real-time polymerase chain reaction,qPCR）检测海马BDNF-TrkB的mRNA与蛋白表达水平。结果：与空白组相比，模型组的睡眠潜伏期（SL）延长、睡眠持续时间（ST）缩短，水平运动距离与垂直探索次数均减少，说明造模成功。与模型组相比，两针刺组的SL缩短、ST延长，水平运动距离与垂直探索次数均增多（均P＜0.05）。与常规针刺组相比，头腹针刺组的SL缩短、垂直探索次数增多（均P＜0.05）。与空白组相比，模型组BDNF、TrkB的mRNA与蛋白表达降低（均P＜0.01）。与模型组相比，两针刺组BDNF、TrkB的mRNA与蛋白表达增多（均P＜0.05）。与常规针刺组相比，头腹针刺组BDNF的mRNA表达增多、BDNF、TrkB的蛋白表达增多（P＜0.05）。结论：孙氏头腹针与常规针刺均能改善焦虑性失眠大鼠的睡眠质量和焦虑样状态，促进BDNF、TrkB的mRNA与蛋白表达，其中孙氏头腹针的疗效更优，为临床治疗焦虑性失眠提供新的治疗思路。
Abstract:
Objective:To observe effect of Sun’s head-abdominal acupuncture on the BDNF-TrkB signaling pathway expression in rats with anxiety-induced insomnia.Methods:SD rats were randomly divided into control group,model group,conventional acupuncture group,and head-abdominal acupuncture group.Except for the control group,anxiety-insomnia models were established in the other three groups using sleep deprivation,anxiety-inducing psychological stress,and intraperitoneal injection of para-chlorophenylalanine.The conventional acupuncture group received standard acupoint treatment,while the head-abdominal acupuncture group received combined head and abdominal acupuncture with electroacupuncture (2 Hz continuous wave,0.5 mA) applied to head acupoints,30 minutes daily over one week.Behavioral assessments,including the pentobarbital-induced sleep test and open field test (OFT),were conducted.Additionally,Western blot (WB) and quantitative polymerase chain reaction (qPCR) were employed to detect hippocampal BDNF-TrkB mRNA and protein expression levels.Results:Compared to the control group,the model group exhibited prolonged sleep latency (SL),shortened sleep duration (ST) (P＜0.01),and reduced horizontal/vertical movement in OFT (all P＜0.05),confirming successful modeling.Both acupuncture groups showed shortened SL,prolonged ST (P＜0.01),and increased motor activity (P＜0.05) compared to the model group.The head-abdominal acupuncture group further outperformed the conventional group in SL reduction and vertical exploration (P＜0.05).Compared with the control group,the model group showed significantly decreased mRNA and protein expression of BDNF and TrkB (P＜0.01).Both acupuncture groups exhibited upregulated BDNF-TrkB expression (P＜0.05),with the head-abdominal group showing higher BDNF and TrkB protein levels than the conventional group (P＜0.05).Conclusion:Both Sun’s head-abdominal acupuncture and conventional acupuncture can improve sleep quality,alleviate anxiety-like behaviors,and enhance BDNF-TrkB signaling in anxiety-insomnia rats.Notably,Sun’s head-abdominal acupuncture shows superior therapeutic efficacy,providing a novel clinical treatment strategy for anxiety-induced insomnia.

参考文献/References:

[1]陈雨晨,车立娟,彭云,等.益神舒眠方治疗失眠症伴焦虑状态疗效研究[J].陕西中医,2025,46(7):908-913.
[2]张艾欣,崔建秋,尹凡.我国失眠人群现状调查及助眠APP市场开发建议[J].中国市场,2021,33(23):96-98.
[3]SUH S,CHO N,ZHANG J.Sex differences in insomnia:From epidemiology and etiology to intervention[J].Curr Psychiatry Rep,2018,20(9):69.
[4]刘华,范小会,毕巧莲,等.针灸为主综合疗法治疗焦虑性失眠的临床观察[J].中国中医基础医学杂志,2020,26(11):1686-1689.
[5]CASTREN E,MONTEGGIA L M.Brain-derived neurotrophic factor signaling in depression and antidepressant action[J].Biol Psychiatry,2021,90(2):128-136.
[6]WANG H,XU J,LAZAROVICI P,et al.cAMP response element-binding protein:A possible signaling molecule link in the pathophysiology of schizophrenia[J].Front Mol Neurosci，2018，30（8）：255.
[7]肖成荣,马增春,李海静,等.PCPA失眠大鼠模型的制作及其机制[J].毒理学杂志,2007,21(4):326-326.
[8]张露芬.实验针灸学[M].北京:化学工业出版社,2010:219-221.
[9]华兴邦,周浩良.大鼠穴位图谱的研制[J].实验动物与动物实验,1991，11(1):5.
[10]孙申田.孙申田针灸治验[M].北京：人民卫生出版社,2013：192-195.
[11]刘晓荻,薛惠文.失眠会增加心脏病和卒中风险[J].基础医学与临床,2020,40(4):537.
[12]MOITRA M,SANTOMAURO D,DEGENHARDT L,et al.Estimating the risk of suicide associated with mental disorders:A systematic review and meta-regression analysis[J].Journal of Psychiatric Research,2021,137:242-249.
[13]谭志康.不同针灸疗法治疗焦虑性失眠疗效比较的网状Meta分析研究[D].南宁：广西中医药大学,2025.
[14]赵东雪.不同频率电针刺激情感区改善卒中后认知障碍执行功能及对定量脑电图影响的临床研究[D].哈尔滨：黑龙江中医药大学,2024.
[15]姚舜,姚凤祯.电针额区腧穴治疗中风后焦虑的临床观察[J].针灸临床杂志,2010,26(4):35-37.
[16]RIEMANN D,SPIEGELHALDER K,NISSEN C,et al.REM sleep instability-a new pathway for insomnia[J].Pharmacopsychiatry,2012,45(5):167-176.
[17]杨婉晴,张春芳,师健,等.应用“经颅重复刺激”针法治疗失眠的经验[J].中华养生保健,2024,42(15):158-161.
[18]VAN SOMEREN E.Brain mechanisms of insomnia:New perspectives on causes and consequences[J].Physiol Rev,2021,101(3):995-1046.
[19]习界一.孙氏头腹针结合治疗失眠伴焦虑的临床疗效观察[D].长春：长春中医药大学,2024.
[20]王丽芹,高兆虹,王飞,等.孙氏腹针治疗肝气郁结型广泛性焦虑症患者的临床观察[J].现代生物医学进展,2020,20(13):2475-2478.
[21]刘婷婷.孙氏腹针区埋线对慢性失眠患者血清神经肽Y影响的研究[D].哈尔滨：黑龙江中医药大学，2020.
[22]MILKA,PEROVIC,VESNA.BDNF transcripts proBDNF and proNGF in the cortex and hippocampus throughout the life span of the rat[J].AGE,2013：257-260.
[23]NUMAKAWA T,ODAKA H,ADACHI N.Actions of Brain-derived neurotrophin factor in the neurogenesis and neuronal function and its involvement in the pathophysiology of brain diseases[J].International Journal of Molecular Sciences,2018,19(11):3650.
[24]NUMAKAWA T,ODAKA H,ADACHI N.Actions of Brain-derived neurotrophin factor in the neurogenesis and neuronal function and its involvement in the pathophysiology of brain diseases[J].International Journal of Molecular Sciences,2018,19(11):3650.
[25]YANG P,CHEN H,WANG T,et al.Electroacupuncture promotes synaptic plasticity in rats with chronic inflammatory pain-related depression by upregulating BDNF/TrkB/CREB signaling pathway[J].Brain and Behavior,2023,13(12): 14.
[26]COLUCCI-D'AMATO L,SPERANZA L,VOLPICELLI F.Neurotrophic factor BDNF physiological functions and therapeutic potential in depression neurodegeneration and brain cancer[J].International Journal of Molecular Sciences,2020,21(20): 7777.
[27]BERGAMI M,BERNINGER B,CANOSSA M.Conditional deletion of TrkB alters adult hippocampal neurogenesis and anxiety-related behavior[J].Communicative and Integrative Biology,2009,2(1): 14-16.

备注/Memo

备注/Memo:
国家重点研发计划“常见多发病防治研究”重点专项课题（2022YFC2503900）；北京中医药大学新教师启动基金资助项目（2023-JYB-XJSJJ058）
更新日期/Last Update: 2025-11-04