[1]雍晨,谭龙旺,陈斐,等.电针干预GDNF基因修饰神经干细胞对大鼠脊髓损伤后Caspase-8表达的影响[J].陕西中医,2026,(8):1011-1017.[doi:DOI:10.3969/j.issn.1000-7369.2026.08.001]
　YONG Chen,TAN Longwang,CHEN Fei,et al.Effect of electroacupuncture intervention on GDNF gene-modified neural stem cells on expression of Caspase-8 in rats after spinal cord injury[J].,2026,(8):1011-1017.[doi:DOI:10.3969/j.issn.1000-7369.2026.08.001]
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电针干预GDNF基因修饰神经干细胞对大鼠脊髓损伤后Caspase-8表达的影响

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2026年8期
页码:
1011-1017
栏目:
基础研究
出版日期:
2026-08-05

文章信息/Info

Title:
Effect of electroacupuncture intervention on GDNF gene-modified neural stem cells on expression of Caspase-8 in rats after spinal cord injury
作者:
雍晨; 谭龙旺; 陈斐; 刘闯; 陈仕强; 王国毓; 李江
(陕西中医药大学第一临床医学院，陕西 咸阳 712046)
Author(s):
YONG Chen; TAN Longwang; CHEN Fei; LIU Chuang; CHEN Shiqiang; WANG Guoyu; LI Jiang
（The First Clinical Medical College of Shaanxi University of Chinese Medicine,Xianyang 712046,China）
关键词:
脊髓损伤; 神经干细胞; 电针; 胶质细胞源性神经营养因子; 细胞凋亡
Keywords:
Spinal cord injury; Neural stem cells; Electroacupuncture; Glial cell line-derived neurotrophic factor; Apoptosis
分类号:
R 744
DOI:
DOI:10.3969/j.issn.1000-7369.2026.08.001
文献标志码:
A
摘要:
目的：探讨高纯度神经干细胞（NSCs）的体外分离培养方法及其生物学特性，评价电针联合胶质细胞源性神经营养因子（GDNF）基因修饰NSCs移植对脊髓损伤后细胞凋亡及大鼠运动功能的干预效应。方法：分离胎鼠脑组织体外培养NSCs，采用携带GDNF基因的慢病毒进行感染并评估感染效率及细胞生长状态。选取60只成年雄性SD大鼠建立T11节段脊髓不完全横断损伤模型，随机分为正常组、模型组、NSCs移植组和电针干预组。采用BBB评分动态评价后肢运动功能恢复。于不同时间点取材，采用免疫组织化学法及蛋白质免疫印迹检测胱天蛋白酶-8（Caspase-8）表达水平。结果：成功建立稳定的NSCs体外培养体系，原代细胞具有良好的增殖能力及多向分化潜能。行为学评价显示，与模型组比较，NSCs移植组及电针干预组BBB评分均呈升高趋势，但两组间差异无统计学意义（P＞0.05）。凋亡指标检测显示，与模型组相比，NSCs移植组及电针干预组Caspase-8表达水平均显著下调（P＜0.05），且电针干预组下降幅度更大（P＜0.05）。结论：体外培养的NSCs在传代2～3代内仍可维持稳定的增殖及分化潜能。GDNF基因修饰NSCs移植能有效抑制脊髓损伤后细胞凋亡，而电针在此基础上可进一步增强抗凋亡作用，但改善运动功能的作用尚未显示出明显优势。
Abstract:
Objective:To explore methods for in vitro isolation and culture of high-purity neural stem cells (NSCs) and their biological characteristics,and to evaluate intervention effects of electroacupuncture combined with glial cell line-derived neurotrophic factor (GDNF) gene-modified NSC transplantation on cell apoptosis and motor function in rats after spinal cord injury.Methods:Fetal mouse brain tissues were isolated for in vitro NSC culture,and lentivirus carrying the GDNF gene was used for infection to assess transfection efficiency and cell growth status.T11 segment spinal cord incomplete transection model was established in 60 adult male SD rats,which were randomly divided into normal,model,NSC transplantation,and electroacupuncture intervention groups.BBB score was used to dynamically evaluate hindlimb motor function recovery.Samples were collected at different time points for assessment of Caspase-8 expression using immunohistochemistry and western blot.Results:A stable in vitro NSC culture system was successfully established,and primary cells showed good proliferation ability and multipotent differentiation potential.Behavioral evaluation showed that both NSC transplantation group and electroacupuncture intervention group had increased BBB scores compared with the model control group,and difference between the two groups was not statistically significant (P＞0.05).Apoptosis marker analysis showed that compared with the model control group,Caspase-8 expression levels were significantly downregulated in both the NSC transplantation group and the electroacupuncture intervention group (P＜0.05),with greater decrease in the electroacupuncture group (P＜0.05).Conclusion:NSCs cultured in vitro can maintain stable proliferation and differentiation potential within passages 2-3.GDNF gene-modified NSC transplantation can effectively inhibit cell apoptosis after spinal cord injury.Electroacupuncture could further enhance anti-apoptotic effect,although no significant advantage in improving motor function has been observed yet.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（81774349）；陕西省科技厅项目（2018SF-348）;陕西省中医药管理局项目（2021-ZZ-LC027);陕西省重点研发计划项目（2023-YBSF-526）;中医药传承创新暨“秦药”开发重点科学研究项目（2021-03-ZZ-008）；陕西省咸阳市科学技术局重点研发项目（L2023-ZDYF-SF-045）
更新日期/Last Update: 2026-08-13