[1]袁博,付慧玲,李国花,等.宁神安脏饮对失眠大鼠下丘脑生物钟基因表达的影响[J].陕西中医,2026,(8):1030-1038.[doi:DOI:10.3969/j.issn.1000-7369.2026.08.004]
　YUAN Bo,FU Huiling,LI Guohua,et al.Effects of Ningshen Anzang Yin on expression of hypothalamic circadian clock genes in insomnia model rats[J].,2026,(8):1030-1038.[doi:DOI:10.3969/j.issn.1000-7369.2026.08.004]
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宁神安脏饮对失眠大鼠下丘脑生物钟基因表达的影响

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2026年8期
页码:
1030-1038
栏目:
基础研究
出版日期:
2026-08-05

文章信息/Info

Title:
Effects of Ningshen Anzang Yin on expression of hypothalamic circadian clock genes in insomnia model rats
作者:
袁博1; 付慧玲2; 李国花1; 李东升1; 贾孟辉2; 王晓丽2
（1.宁夏医科大学，宁夏 银川 750004；2.银川市第一人民医院 宁夏医科大学第二临床医学院，宁夏 银川750001）
Author(s):
YUAN Bo1; FU Huiling2; LI Guohua1; LI Dongsheng1; JIA Menghui2; WANG Xiaoli2
(1.Ningxia Medical University,Yinchuan 750004,China；2.Yinchuan First People’s HospitalThe Second Clinical Medical College of Ningxia Medical University,Yinchuan 750001,China)
关键词:
失眠; 宁神安脏饮; 生物钟基因; 昼夜节律; 下丘脑; 睡眠障碍
Keywords:
Insomnia; Ningshen Anzang Yin; Circadian clock genes; Circadian rhythm; Hypothalamus; Sleep disorders
分类号:
R 256.23
DOI:
DOI:10.3969/j.issn.1000-7369.2026.08.004
文献标志码:
A
摘要:
目的：探讨宁神安脏饮对失眠模型大鼠下丘脑生物钟基因表达的影响。方法：80只SD大鼠随机分为空白组、模型组、宁神安脏饮组及艾司唑仑组，每组20只。除空白组外，其余组采用慢性不可预知温和刺激（CUMS）联合腹腔注射对氯苯丙氨酸（PCPA）建立失眠模型。宁神安脏饮组予2.36 g/(kg·d)灌胃，艾司唑仑组予0.32 mg/(kg·d)灌胃，空白组及模型组予等体积0.9%氯化钠溶液，每日1次，持续14 d。观察大鼠一般状态及体重；戊巴比妥钠协同实验检测睡眠潜伏期与睡眠持续时间；HE染色观察下丘脑神经元形态；qRT-PCR检测下丘脑CLOCK、BMAL1、Per1、Cry1 mRNA表达；Western blot及免疫组化检测相应蛋白表达。结果：与空白组比，模型组大鼠一般状态差，体重降低（P＜0.05），睡眠时间缩短，下丘脑神经元空泡样变。与模型组比，宁神安脏饮组及艾司唑仑组一般状态改善，体重增加（P＜0.05），睡眠持续时间延长，神经元形态改善。模型组CLOCK、BMAL1 mRNA及蛋白表达升高，Per1、Cry1 mRNA及蛋白降低（P＜0.01）。两给药组均逆转上述异常（P＜0.05）。宁神安脏饮组逆转CLOCK、Per1、Cry1蛋白表达（P＜0.01），艾司唑仑组改善CLOCK、Per1蛋白表达（P＜0.01）。免疫组化显示两给药组均改善CLOCK、BMAL1、Per1、Cry1阳性表达（P＜0.01）。结论：宁神安脏饮可改善失眠模型大鼠行为状态及睡眠质量，其机制可能与调节下丘脑生物钟基因表达有关。
Abstract:
Objective:To investigate effects of Ningshen Anzang Yin on expression of hypothalamic circadian clock genes in insomnia model rats.Methods:Eighty Sprague-Dawley(SD) rats were randomly divided into four groups blank control group,model group,Ningshen Anzang Yin group,and eszopiclone group,with 20 rats in each group.Except for the blank control group,the other groups were subjected to chronic unpredictable mild stress(CUMS) combined with intraperitoneal injection of para-chlorophenylalanine(PCPA) to establish an insomnia model.The Ningshen Anzang Yin group received 2.36 g/(kg·d) by gavage,the eszopiclone group received 0.32 mg/(kg·d) by gavage,while the blank and model group received an equal volume of 0.9% saline solution daily for 14 consecutive days.General condition and body weight were observed.Sleep latency and duration were assessed using pentobarbital sodium-induced sleep tests.Neuronal morphology in the hypothalamus was examined via hematoxylin-eosin(HE) staining.mRNA expression levels of CLOCK,BMAL1,Per1,and Cry1 in the hypothalamus were detected by qRT-PCR.Protein expression was analyzed by Western blot and immunohistochemistry.Results:Compared with the blank control group,model rats showed poor general condition,reduced body weight(P＜0.05),shortened sleep duration,and vacuolar degeneration of hypothalamic neurons.Compared with the model group,both the Ningshen Anzang Yin and eszopiclone group exhibited improved general condition,increased body weight(P＜0.05),prolonged sleep duration,and ameliorated neuronal morphology.In the model group,mRNA and protein expression of CLOCK and BMAL1 was elevated,whereas that of Per1 and Cry1 was decreased(P＜0.01).Both treatment groups reversed these abnormalities(P＜0.05).The Ningshen Anzang Yin group significantly reversed the protein expression of CLOCK,Per1,and Cry1(P＜0.01),while the eszopiclone group improved CLOCK and Per1 protein expression(P＜0.01).Immunohistochemical analysis revealed enhanced positive expression of CLOCK,BMAL1,Per1,and Cry1 in both treated groups(P＜0.01).Conclusion:Ningshen Anzang Yin can improve behavioral status and sleep quality in insomnia model rats,possibly through regulation of hypothalamic circadian clock gene expression.

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备注/Memo

备注/Memo:
宁夏回族自治区自然科学基金资助项目（2024AAC03795）；第七批全国老中医药专家学术经验继承项目（国中医教函〔2022〕76号）；宁夏回族自治区青年拔尖人才培养项目（宁人社函〔2026〕33号）
更新日期/Last Update: 2026-08-13