[1]俞 燕,郑艳辉,田海军.六月雪对糖尿病肾病大鼠肾脏的保护作用研究[J].陕西中医,2023,(1):12-15,28.[doi:DOI:10.3969/j.issn.1000-7369.2023.01.003]
　YU Yan,ZHENG Yanhui,TIAN Haijun.Protective effect of Serissa serissoides(DC.)Druce(SSD)on diabetic nephropathy rats[J].,2023,(1):12-15,28.[doi:DOI:10.3969/j.issn.1000-7369.2023.01.003]
点击复制

六月雪对糖尿病肾病大鼠肾脏的保护作用研究
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年1期
页码:
12-15,28
栏目:
基础研究
出版日期:
2023-01-05

文章信息/Info

Title:
Protective effect of Serissa serissoides(DC.)Druce(SSD)on diabetic nephropathy rats
作者:
俞 燕1; 郑艳辉2; 田海军1
(1.南京中医药大学,江苏 南京 210023; 2.南京中医药大学附属南京中医院,江苏 南京 210001)
Author(s):
YU Yan; ZHENG Yanhui; TIAN Haijun
(Nanjing University of Chinese Medicine,Nanjing 210023,China)
关键词:
糖尿病肾病;  肾脏;  六月雪;  保护作用;  炎症反应;  氧化应激
Keywords:
Diabetic nephropathy;  Kidney;  Serissa serissoides(DC.)Druce(SSD);  Protective effects;  Inflammation;  Oxidative stress
分类号:
R 587.2
DOI:
DOI:10.3969/j.issn.1000-7369.2023.01.003
文献标志码:
A
摘要:
目的:研究六月雪对糖尿病肾病大鼠肾脏的保护作用。方法:将30只SD大鼠随机分为正常组、模型组及六月雪组,每组10只,通过腹腔注射链脲佐菌素的方法建立糖尿病肾病大鼠模型。干预6周后,比较各组大鼠的空腹血糖(FBG)、尿素氮(BUN)、血肌酐(Scr)及24 h尿蛋白定量(24 h-UP); HE染色及PAS染色观察肾脏组织病理的变化,计算并比较各组病理评分; ELISA法检测各组大鼠肾脏组织中TGF-β1及IL-6的表达水平并进行比较。结果:六月雪组大鼠的FBG、BUN、Scr及24 h-UP较模型组降低(P<0.01); 与模型组比较,六月雪组大鼠的肾脏病理改变有所减轻,病理评分降低(P<0.01); 与模型组比较,六月雪组大鼠TGF-β1及IL-6表达有所下降(P<0.05)。结论:六月雪能够改善肾功能,减少尿蛋白,减轻肾脏损伤,其肾脏保护作用可能与TGF-β1及IL-6的表达下调有关。
Abstract:
Objective:To study the protective effect of Serissa serissoides(DC.)Druce(SSD)on diabetic nephropathy rats.Methods:Thirty rats were randomly divided into normal group,model group and SSD group.Each group has 10 rats.Diabetic nephropathy rat model was established by STZ.After intervention for 6 weeks,fasting blood glucose(FBG),urea nitrogen(BUN),serum creatinine(Scr)and 24 h-urinary protein(24 h-UP)were compared in each group.Pathological changes of kidney tissue were observed by HE and PAS staining,and pathological score was also compared.TGF-β1 and IL-6 in kidney tissue were detected by ELISA tissue.Results:The results showed that compared with the model group,the SSD group can decrease the FBG,Scr,BUN and 24 h-UP(P<0.01).In terms of renal histopathology,we can see the SSD group can improve the kidney pathological changes of rats and reduce the pathological score(P<0.01).The level of TGF-β1 and IL-6 can be reduced(P<0.05).Conclusion:SSD can protect renal function,reduce urinary protein and improve kidney damage.And its protective effects on renal may be related to the reduced expression of TGF-β1 and IL-6.

参考文献/References:

[1] 艾思南,朱泽兵,郑慧娟,等.基于网络药理学分析当归补血汤治疗糖尿病肾脏病的内在机制[J].世界中医药,2021,16(15):2254-2260.
[2] Gnudi L,Coward R,Long DA.Diabetic nephropathy:Perspective on novel molecular mechanisms[J].Trends in Endocrinology & Metabolism Tem,2016,30(2):820-830.
[3] 布天杰,潘雨烟,张 宁.真武汤治疗糖尿病肾病作用机制的网络药理学研究[J].世界中医药,2020,15(24):3719-3727.
[4] 焦 杨,丁石梅,张 静,等.丹参川芎嗪联合贝前列素钠片治疗早期糖尿病肾病临床研究[J].陕西中医,2020,41(7):907-910.
[5] 贾会玉,那 莎,李 莉,等.丹蛭降糖胶囊对大鼠糖尿病肾病防治作用的研究[J].中华中医药杂志,2016,31(12):5244-5247.
[6] Li X,Zhu Q,Zheng R,et al.Puerarin attenuates diabetic nephropathy by promoting autophagy in podocytes[J].Frontiers in Physiology,2020,11:73.
[7] 林子桐,张 超,沈雪梅.糖尿病肾病发病机制研究进展[J].中国药理学与毒理学杂志,2014,28(5):765-773.
[8] Stieger N,Worthmann K,Teng B,et al.Impact of high glucose and transforming growth factor-β on bioenergetic profiles in podocytes[J].Metabolism-Clinical & Experimental,2012,61(8):1073-1086.
[9] Tang R,Xiao X,Lu Y,et al.Interleukin-22 attenuates renal tubular cells inflammation and fibrosis induced by TGF-β1 through Notch1 signaling pathway[J].Renal Failure,2020,42(1):381-390.
[10] 李 静,常 盼,王西辉,等.血清晚期蛋白氧化产物联合转化生长因子β检测在高血压肾病中的诊断价值[J].陕西医学杂志,2021,50(1):26-28,47.
[11] 马 旭,朱桂珍,朱 清,等.脑心通联合厄贝沙坦治疗糖尿病肾病疗效及对血清TGF-β、MMP-9、TNF-α影响的研究[J].陕西中医,2018,39(12):1715-1718.
[12] Meng XM,Tang MK,Li J,et al.TGF-β/Smad signaling in renal fibrosis[J].Frontiers in Physiology,2015,6:82.
[13] 江 丹,汪 何,姚 楠,等.基于TGF-β1/p38MAPK通路探讨益气升清方对糖尿病大鼠肾组织的影响[J].世界中医药,2020,15(16):2381-2386.
[14] 段 颖,郭翔宇,刘铜华,等.番石榴叶抑制db/db小鼠肾脏TGF-β1蛋白表达研究[J].陕西中医,2018,39(7):819-822.
[15] Su L,Cao P,Wang H.Tetrandrine mediates renal function and redox homeostasis in a streptozotocin-induced diabetic nephropathy rat model through Nrf2/HO-1 reactivation[J].Annals of Translational Medicine,2020,8(16):990-993.
[16] Kelly KJ,Dominguez JH.Treatment of the post-ischaemic inflammatory syndrome of diabetic nephropathy[J].Nephrol Dial Transplant,2010(10):3204-3212.
[17] 张晓丽.血液透析联合血液灌注对糖尿病肾病患者血清空腹血糖、 C反应蛋白、白细胞介素-6影响的临床研究[J].陕西医学杂志,2020,49(1):68-71.
[18] Sta B,Mtd A,Nws A.Crosstalk between cellular metabolism and histone acetylation[J].Methods in Enzymology,2019,626:1-21.
[19] 高大红.血府逐瘀胶囊对糖尿病肾病蛋白尿患者临床疗效与炎症因子水平的影响[J].陕西中医,2018,39(3):361-364.
[20] 冯 颖,苏衍进,司海龙,等.水陆地黄汤联合盐酸贝那普利对气阴两虚夹瘀型糖尿病肾病氧化应激及临床指标的影响[J].陕西中医,2020,41(5):605-608.
[21] 林兴生,林占熺,林冬梅,等.菌草作为生物质燃料的初步研究[J].福建林学院学报,2013,33(1):82-86.
[22] 许玉华,甄丹丹,甄汉深.六月雪的研究进展[J].中国民族民间医药,2016,25(22):24-26,30.
[23] 鲁利民,陆锦锐,韩忠耀.苗药六月雪化学成分及药理作用研究进展[J].中国中医药现代远程教育,2014,12(17):158-160.
[24] 孙响波,于妮娜.六月雪治疗肾脏疾病探源[J].中医药导报,2013,19(10):127-128.

相似文献/References:

[1]张华贞.复方黄芪汤加减联合骨化三醇治疗糖尿病肾病疗效及对患者血糖、肾功的影响*[J].陕西中医,2019,(6):767.
[2]程红卫,成晓萍.阎晓萍从脾论治糖尿病肾病经验总结*[J].陕西中医,2019,(6):790.
[3]黄聪丽,王治新△.益气滋肾活血通络方治疗早中期糖尿病肾病临床研究[J].陕西中医,2019,(10):1390.
　HUANG Congli,WANG Zhixin..Clinical efficacy analysis of Yiqi Zishen Huoxue Tongluo decoctionon early and medium stage of diabetic nephropathy[J].,2019,(1):1390.
[4]玉山江·艾克木,哈丽达·木沙△,地丽尼嘎尔.西红康联合还原型谷胱甘肽治疗2型糖尿病肾病Ⅲ期气阴两虚夹瘀证疗效及对患者微炎症状态、抗氧化的影响*[J].陕西中医,2020,(3):338.
　Yushanjiang·Aikemu,Halida·Musha,Dilinigaer..Efficacy of adjuvant therapy of Xihongkang on type 2 diabetic nephropathy Ⅲ phase with syndrome of Qiyin Liangxu Jiayu type and its effect on micro inflammation and antioxidant role[J].,2020,(1):338.
[5]马民凯,马红建,陈新苗.通络散结汤对3期糖尿病肾病患者GFR、UAER、β2-MG、Cys-C及微炎症状态的影响*[J].陕西中医,2020,(10):1432.[doi:DOI:10.3969/j.issn.1000-7369.2020.10.025]
[6]田娟娟,陈 强,周欣欣,等.益气养阴活血法治疗糖尿病肾病理论探究*[J].陕西中医,2020,(12):1781.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.026]
[7]代广玉,修丽梅.糖尿病肾病中医辨证分型研究进展[J].陕西中医,2021,(1):131.[doi:DOI:10.3969/j.issn.1000-7369.2020.01.036]
[8]赵 亚,张勉之,樊威伟,等.国医大师张大宁治疗糖尿病肾病用药经验[J].陕西中医,2021,(6):773.[doi:DOI:10.3969/j.issn.1000-7369.2020.06.025]
[9]张 蕊,王子承,蒋荣莉,等.防己黄芪汤加味治疗糖尿病肾病疗效及对患者糖脂代谢、氧化应激的影响[J].陕西中医,2021,(8):1049.[doi:DOI:10.3969/j.issn.1000-7369.2020.08.015]
　ZHANG Rui,WANG Zicheng,JIANG Rongli,et al.Effect of Fangji Huangqi decoction on diabetic nephropathy and its influence on glucose and lipid metabolism and oxidative stress[J].,2021,(1):1049.[doi:DOI:10.3969/j.issn.1000-7369.2020.08.015]
[10]方锦颖,王一冲,神原优滋(日本),等.基于网络药理学和分子对接探究肾康注射液治疗糖尿病肾病作用机制[J].陕西中医,2021,(12):1798.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.039]

备注/Memo

备注/Memo:
基金项目:南京市科学技术委员会基金资助项目(201803018)
更新日期/Last Update: 2022-12-28