[1]郭红梅,王新国,李志鹏,等.扶正消萎汤治疗慢性萎缩性胃炎大鼠机制研究[J].陕西中医,2025,46(6):729-733,740.[doi:DOI:10.3969/j.issn.1000-7369.2025.06.002]
　GUO Hongmei,WANG Xinguo,LI Zhipeng,et al.In vivo study on therapeutic effect and mechanism of Fuzheng Xiaowei decoction in treatment of chronic atrophic gastritis[J].,2025,46(6):729-733,740.[doi:DOI:10.3969/j.issn.1000-7369.2025.06.002]
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扶正消萎汤治疗慢性萎缩性胃炎大鼠机制研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年6期
页码:
729-733,740
栏目:
基础研究
出版日期:
2025-06-05

文章信息/Info

Title:
In vivo study on therapeutic effect and mechanism of Fuzheng Xiaowei decoction in treatment of chronic atrophic gastritis
作者:
郭红梅1; 2; 王新国1; 李志鹏3; 叶文俊1; 马雅心4
（1.新疆维吾尔自治区中医医院脾胃病科，新疆 乌鲁木齐 830000；2.新疆维吾尔自治区中医药研究院，新疆 乌鲁木齐 830001； 3.新疆维吾尔自治区中西医结合医院老年病科,新疆 乌鲁木齐 830002；4.新疆医科大学中医学院,新疆 乌鲁木齐 830011）
Author(s):
GUO Hongmei1; 2; WANG Xinguo1; LI Zhipeng3; YE Wenjun1; MA Yaxin4
(1.Department of Spleen and Stomach Diseases,Xinjiang Uygur Autonomous Region Hospital of Traditional Chinese Medicine,Urumqi 830000,China;2.Xinjiang Uygur Autonomous Region Institute of Traditional Chinese Medicine,Urumqi 830001,China;3.Department of Geriatrics,Xinjiang Uygur Autonomous Region Hospital of Integrated Chinese and western Medicine,Urumqi 830002,China;4.College of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830011,China)
关键词:
慢性萎缩性胃炎; 扶正消萎汤; 益气健脾法; 肿瘤坏死因子-α; 干扰素-γ; 胃黏膜结构; 炎症反应; 氧化应激
Keywords:
Chronic atrophic gastritis; Fuzheng Xiaowei decoction; Method of nourishing Qi and strengthening spleen; Tumor necrosis factor-α; Interferon-γ; Gastric mucosal structure; Inflammatory reaction; Oxidative stress
分类号:
R 573.3
DOI:
DOI:10.3969/j.issn.1000-7369.2025.06.002
文献标志码:
A
摘要:
目的：探讨扶正消萎汤对慢性萎缩性胃炎（CAG）的影响及潜在机制。方法：建立CAG大鼠模型,随机分为正常对照组、CAG组、阳性药物组以及扶正消萎汤低、中、高剂量组。采用HE染色、试剂盒测定法、酶联免疫吸附试验、荧光定量聚合酶链反应（PCR）、Western blot等方法观察胃黏膜形态学改变，并检测相关生化指标、炎症因子含量、氧化指标、α-平滑肌肌动蛋白（α-SMA）和转化生长因子-β1（TGF-β1）基因和蛋白表达变化。结果：与正常对照组比较，CAG组局灶黏膜层变薄、固有层基底部腺体减少，胃黏膜呈萎缩性改变；血清钙（Ca）降低，尿素氮（BUN）、肌酐（Cr）和磷（Pi）升高；血清白介素（IL）-10水平降低，IL-6、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）和IL-1β水平升高；血清超氧化物歧化酶（SOD）降低，丙二醛（MDA）水平升高；α-SMA和TGF-β1的mRNA和蛋白表达升高（均P＜0.05）。与CAG组比较，各治疗组胃黏膜层厚度明显增加，腺体数量恢复，胃黏膜萎缩性改变得到改善；血清Ca升高，BUN、Cr和Pi降低；血清IL-10升高，IL-6、TNF-α、IFN-γ和IL-1β降低；血清SOD升高，MDA水平降低；α-SMA和TGF-β1的mRNA和蛋白表达降低（均P＜0.05）。治疗组间对比提示扶正消萎汤高剂量组变化尤为显著（均P＜0.05）。结论：扶正消萎汤能有效改善胃黏膜结构、生化指标、炎症反应和氧化应激，降低α-SMA和TGF-β1表达水平。
Abstract:
Objective:To study the effect and potential mechanism of the Fuzheng Xiaowei decoction on chronic atrophic gastritis(CAG).Methods:This study established CAG rats model and randomly divide the modeled rats into a normal control group,CAG group,positive drug group,and low,moderate and high-dose intervention group of Fuzheng Xiaowei decoction.Furthermore,this study adopted HE staining,kit assay,enzyme-linked immunosorbent assay,fluorescence quantitative polymerase chain reaction(PCR),Western blot and other methods to observe the morphology of gastric mucosa,and to detect related biochemical indicators,inflammatory factor content,oxidative indicators,α-smooth muscle actin (α-SMA) and transforming growth factor-β1(TGF-β1) gene and protein expression changes.Results:Compared with the normal control group,CAG group had localized thinning of gastric mucosa,reduction of basal glandular tissue in the lamina propria,and atrophic changes in gastric mucosa;decreased serum calcium (Ca),while increased blood urea nitrogen(BUN),creatinine (Cr),and phosphorus (Pi);reduced serum interleukin (IL)-10 level,but increased IL-6,tumor necrosis factor-α (TNF-α),interferon-γ (IFN-γ),and IL-1β levels;decreased serum superoxide dismutase (SOD),and increased malondialdehyde (MDA) level;as well as increased α-SMA and TGF-β1 at mRNA and protein levels (all P<0.05).Furthermore,compared with CAG group,all treatment groups showed significantly increased gastric mucosal thickness,restored number of glands,and improved atrophic changes in the gastric mucosa;increased serum Ca,while decreased BUN,Cr and Pi;elevated serum IL-10 level,but reduced IL-1β and TNF-α levels;increased serum SOD,and decreased MDA level;as well as reduced α-SMA and TGF-β1 at mRNA and protein levels (all P<0.05).Moreover,the comparison among treatment groups suggested that the high-dose intervention group of Fuzheng Xiaowei decoction showed particularly significant changes (all P<0.05).Conclusion:Fuzheng Xiaowei decoction can effectively improve gastric mucosal structure,biochemical indicators,inflammatory response and oxidative stress,while reduce expression levels of α-SMA and TGF-β1.

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备注/Memo

备注/Memo:
新疆维吾尔自治区自然科学基金资助项目（2022D01C164）
更新日期/Last Update: 2025-06-09