[1]李佩云,苑功名,徐媛,等.基于炎性痛模型初探针刺对cGAS/STING信号通路的双向调节效应[J].陕西中医,2025,46(6):746-750.[doi:DOI:10.3969/j.issn.1000-7369.2025.06.005]
　LI Peiyun,YUAN Gongming,XU Yuan,et al.The bidirectional modulatory effects of acupuncture on the cGAS/STING signaling pathway with inflammatory pain[J].,2025,46(6):746-750.[doi:DOI:10.3969/j.issn.1000-7369.2025.06.005]
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基于炎性痛模型初探针刺对cGAS/STING信号通路的双向调节效应
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年6期
页码:
746-750
栏目:
基础研究
出版日期:
2025-06-05

文章信息/Info

Title:
The bidirectional modulatory effects of acupuncture on the cGAS/STING signaling pathway with inflammatory pain
作者:
李佩云1; 苑功名1; 徐媛1; 郭永明1; 萧翊峰2（马来西亚）; 李媛3; 4
（1.天津中医药大学实验针灸学研究中心，天津 301617；2.马来西亚中医总会，吉隆坡 50100；3.南京中医药大学针灸推拿学院，江苏 南京 210023；4.南京中医药大学教育部针药结合重点实验室，江苏 南京 210023）
Author(s):
LI Peiyun1; YUAN Gongming1; XU Yuan1; GUO Yongming1; XIAO Yifeng2; LI Yuan3; 4
(1.Research Center of Experimental Acupuncture Science,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;2.The Malaysian Chinese Medical Association,Kuala Lumpur 50100，Malaysia;3.College of Acupuncture and Massage Health and Rehabilitation College,Nanjing University of Chinese Medicine,Nanjing 210023,China;4.Key Laboratory of Acupuncture and Medicine Research of Ministry of Education,Nanjing University of Chinese Medicine,Nanjing 210023,China)
关键词:
炎性痛; 针刺; cGAS/STING信号通路; 脊髓; 背根神经节; 疼痛机制
Keywords:
Inflammatory pain; Acupuncture; cGAS/STING signaling pathway; Spinal cord; Dorsal root ganglion; Pain mechanism
分类号:
R 441.1
DOI:
DOI:10.3969/j.issn.1000-7369.2025.06.005
文献标志码:
A
摘要:
目的：观察针刺足三里对完全弗氏佐剂（CFA）诱导的炎性痛小鼠环磷酸鸟苷-腺苷酸合成酶（cGAS）/干扰素基因刺激因子（STING）信号通路表达的影响。方法：将C57BL/6J小鼠随机分为盐水组、模型组和针刺组，盐水组右后足底注射0.9%氯化钠溶液，模型组和针刺组右后足底注射CFA造模。针刺组选取足三里穴（ST36）针刺，盐水组与模型组同等固定，每日1次，共7次。测量小鼠热刺激缩足潜伏期（PWTL）、机械刺激缩足阈值（PWMT）以观察各组小鼠疼痛情况；采用RT-qPCR法检测背根神经节（DRG）和脊髓cGAS、STING、干扰素（IFN）-β的mRNA表达水平，结果：造模后，与盐水组相比，模型组小鼠PWTL、PWMT降低（P<0.05）；针刺干预后，与模型组相比，针刺组PWTL、PWMT升高（P<0.05）。与盐水组相比，模型组小鼠DRG内cGAS、STING、IFN-β的mRNA表达水平降低（P<0.05），脊髓cGAS、STING、IFN-β的mRNA表达水平升高（P<0.05）；与模型组相比，针刺组小鼠DRG内cGAS、STING、IFN-β的mRNA表达水平升高（P<0.05），脊髓cGAS、STING、IFN-β的mRNA表达水平下降（P<0.05）。结论：针刺能够改善CFA小鼠炎性痛；且在不同部位（DRG和脊髓）对cGAS/STING信号通路的mRNA表达具有双向调节作用。
Abstract:
Objective:To observe the effect of acupuncture on the expression of cyclic GMP-AMP synthase (cGAS) /stimulator of interferon genes (STING) signaling pathway in the spinal cord of inflammatory pain mice induced by Complete Freund’s Adjuvant (CFA).Methods:C57BL/6J mice were randomly divided into saline group,model group and acupuncture group.In the saline group,normal saline was injected into the right hind paw,and CFA was injected into the same hind paw of the model group and the acupuncture group.Acupuncture was applied to Zusanli (ST36) in the acupuncture group,and the other two groups were equally fixed,once a day for 7 times.Paw Withdrawal Thermal Latency (PWTL),Paw Withdrawal Mechanical Threshold (PWMT) and paw swelling were tested during the experiment.The mRNA expression of cGAS,STING,interferon(IFN)-β in spinal cord and dorsal root ganglion were detected by RT-qPCR.Results:Compared with the saline group,the PWTL and PWMT of model group was decreased (P<0.01).Compared with the model group,the PWTL and PWMT of acupuncture group was increased (P<0.01),until on the 7th day there was statistically difference between the two groups (P<0.01).Compared with the saline group,the mRNA expression of cGAS,STING，IFN-β in the dorsal root ganglion of the model group were decreased (P<0.05)，and the mRNA expression of cGAS,STING，IFN-β in the spinal cord of the model group were increased (P<0.05).Compared with the model group,the expression of the above-mentioned genes of the acupuncture group were decreased (P<0.05).the expression of the above-mentioned genes of the acupuncture group were increased (P<0.05).Conclusion:Acupuncture can improve inflammatory pain in CFA mice;it also has a bidirectional regulatory effect on the mRNA expression of cGAS/STING signaling pathway in DRG and spinal cord.

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备注/Memo

备注/Memo:
国家自然科学基金青年基金资助项目（82004473）；江苏省科技计划项目基础研究计划自然科学基金青年基金资助项目（BK20230453）；江苏省卫生健康委员会医学科研项目（Z2023032）；江苏省高等学校基础科学（自然科学）研究面上省资助经费项目（22KJB360009）
更新日期/Last Update: 2025-06-09