[1]袁红丽,牛相来,肖亚平,等.针刺对营养型肥胖小鼠肾周脂肪组织PHF23基因和Apelin/APJ 信号通路的影响[J].陕西中医,2025,46(9):1166-1171.[doi:DOI:10.3969/j.issn.1000-7369.2025.09.003]
　YUAN Hongli,NIU Xianglai,XIAO Yaping,et al.Effects of acupuncture on PHF 23 gene and Apelin/APJ signal pathway in perirenal adipose tissue of nutritional obese mice[J].,2025,46(9):1166-1171.[doi:DOI:10.3969/j.issn.1000-7369.2025.09.003]
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针刺对营养型肥胖小鼠肾周脂肪组织PHF23基因和Apelin/APJ 信号通路的影响

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年9期
页码:
1166-1171
栏目:
基础研究
出版日期:
2025-09-05

文章信息/Info

Title:
Effects of acupuncture on PHF 23 gene and Apelin/APJ signal pathway in perirenal adipose tissue of nutritional obese mice
作者:
袁红丽; 牛相来; 肖亚平; 李秀娟; 汪秀梅; 周钰
（新疆医科大学第一附属医院针灸推拿科，新疆 乌鲁木齐 830054）
Author(s):
YUAN Hongli; NIU Xianglai; XIAO Yaping; LI Xiujuan; WANG Xiumei; ZHOU Yu
(Department of Acupuncture and Moxibustion and Massage,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)
关键词:
营养型肥胖; 针刺; 肾周脂肪组织; PHF 23; Apelin/APJ 信号通路; 脂肪因子
Keywords:
Nutritional obesity; Acupuncture; Perirenal adipose tissue; PHF23; Apelin/APJ signaling pathway; Adipokine
分类号:
R 723.14
DOI:
DOI:10.3969/j.issn.1000-7369.2025.09.003
文献标志码:
A
摘要:
目的：探讨针刺对营养型肥胖小鼠肾周脂肪组织PHF23基因和Apelin/APJ信号通路的影响机制。方法：选择8周龄C57 BL/6小鼠80只，随机分为对照组、模型组、针刺组和APJ抑制剂组，各20只。对照组正常饮食，其他三组高脂饮食8周复制营养型肥胖模型。针刺组选择中脘、关元、天枢、足三里4个穴位。APJ抑制剂组在针刺前1周于尾静脉注射APJ抑制剂。4周后处死获取肾周脂肪组织，HE染色观察脂肪组织形态，qRT-PCR检测PHF 23基因，Western blot检测Apelin和APJ蛋白。检测T1（喂养前）、T2（喂养8周）、T3（针刺2周）和T4（针刺4周）时刻体重、总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白（LDL）、瘦素和脂联素水平。结果：与对照组比较，模型组肾周脂肪重量和脂肪细胞直径明显增加，而脂肪细胞数目明显减少（P＜0.05）；与模型组比较，针刺组肾周脂肪重量和脂肪细胞直径明显减少，而脂肪细胞数目明显增加（P＜0.05）。与模型组比较，APJ抑制剂组上述改变不明显（P＞0.05）。与对照组比较，模型组PHF23基因、Apelin和APJ蛋白表达量明显增加（P＜0.05）；与模型组比较，针刺组PHF23基因、Apelin和APJ蛋白表达量明显减少（P＜0.05）。与模型组比较，APJ抑制剂组PHF23基因和Apelin蛋白表达量明显减少（P＜0.05），但APJ蛋白表达量改变不明显（P＞0.05）。与对照组比较，模型组T2、T3和T4时刻体重、TC、TG、LDL、瘦素和脂联素水平明显增加（P＜0.05）；与模型组比较，针刺组T3和T4时刻体重、TC、TG、LDL、瘦素和脂联素水平明显减少（P＜0.05）。与模型组比较，APJ抑制剂组上述改变不明显（P＞0.05）。结论：针刺可以明显降低营养型肥胖小鼠体重、血脂和脂肪因子水平，改善肾周脂肪组织的病理变化，可能与抑制PHF23基因以及Apelin/APJ 信号通路的表达有关，拮抗APJ蛋白表达可以抵消针刺的上述作用。
Abstract:
Objective:To explore the mechanism of acupuncture on PHF 23 gene and Apelin/APJ signaling pathway in perirenal adipose tissue of nutritional obese mice.Methods:Eighty 8-week-old healthy SPF C57 BL/6 mice were randomly divided into control group,model group,acupuncture group and APJ inhibitor group,with 20 mice in each group.The control group had normal diet,other three groups had high-fat diet for 8 weeks to duplicate nutritional obesity model.In acupuncture group,four acupoints including Zhongwan,Guanyuan,Tianshu and Zusanli were selected once a day,left and right wheels were exchanged for points.The APJ inhibitor group was injected with APJ inhibitor into tail vein one week before acupuncture.After 4 weeks,the mice were euthanized to obtain perirenal adipose tissue,morphology of adipose tissue was observed by HE staining,PHF 23 gene expression was detected by qRT-PCR,and Apelin and APJ protein expressions were detected by Western blot.Body weight,total cholesterol (TC),triglyceride (TG),low-density lipoprotein (LDL),leptin and adiponectin were measured at T1 (before feeding),T2 (8 weeks after feeding),T3 (2 weeks after acupuncture) and T4 (4 weeks after acupuncture)were detected.Results:Compared with control group,model group showed significant increases in perirenal fat weight and adipocyte diameter,while number of adipocyte decreased significantly (P<0.05).Compared with model group,acupuncture group showed significant decreases in perirenal fat weight and adipocyte diameter,while number of adipocyte increased significantly (P<0.05).Compared with model group,the above changes were not significant in APJ inhibitor group (P>0.05).Compared with control group,the levels of PHF 23 gene,Apelin,and APJ proteins in model group significantly increased (P<0.05).Compared with model group,PHF 23 gene,Apelin and APJ proteins in acupuncture group decreased significantly (P<0.05).Compared with model group,the expression levels of PHF 23 gene,Apelin protein significantly reduced in APJ inhibitor group (P<0.05),but APJ protein expression was not significant (P>0.05).Compared with control group,model group showed significant increases in body weight,levels of TC,TG,LDL,leptin and adiponectin at T2,T3,and T4 times (P<0.05).Compared with model group,acupuncture group showed significant decreases in body weight,levels of TC,TG,LDL,leptin and adiponectin at T3 and T4 times (P<0.05).Compared with model group,the above changes were not significant in APJ inhibitor group (P>0.05).Conclusion:Acupuncture can greatly reduce weight,blood lipid and adipokine levels of nutritional obese mice,improve pathological changes of perirenal adipose tissue,which may be related to inhibition of PHF23 gene and Apelin/APJ signal pathway expressions.Antagonistic APJ protein expression can counteract above effects of acupuncture.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目(81960898);新疆维吾尔自治区自然科学基金资助项目（2022D01C494）
更新日期/Last Update: 2025-09-08