[1]徐匡迪,胡春兰,吴晓珲,等.电针足三里对肝叶部分切除术后小鼠免疫功能及炎症反应的影响[J].陕西中医,2026,(1):10-14.[doi:DOI:10.3969/j.issn.1000-7369.2026.01.002]
　XU Kuangdi,HU Chulan,WU Xiaohui,et al.Electroacupuncture at Zusanli on postoperative immune function and inflammation in mice undergoing partial hepatectomy[J].,2026,(1):10-14.[doi:DOI:10.3969/j.issn.1000-7369.2026.01.002]
点击复制

电针足三里对肝叶部分切除术后小鼠免疫功能及炎症反应的影响

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2026年1期
页码:
10-14
栏目:
基础研究
出版日期:
2026-01-05

文章信息/Info

Title:
Electroacupuncture at Zusanli on postoperative immune function and inflammation in mice undergoing partial hepatectomy
作者:
徐匡迪1; 胡春兰1; 吴晓珲1; 雍玥1; 2; GWEE Kang Cheng3（马来西亚）; 陈文婷1; 高浩1
（1.上海中医药大学附属曙光医院麻醉科，上海 201203；2.上海中医药大学附属曙光医院针麻研究所，上海 201203；3.马来西亚国医堂医药中心，马来西亚 新山 81200）
Author(s):
XU Kuangdi1; HU Chulan1; WU Xiaohui1; YONG Yue1; 2; GWEE Kang Cheng 3; CHEN Wenting1; GAO Hao1
（1.Department of Anesthesiology,Shuguang Hospital Affiliated with Shanghai University of Traditional Chinese Medicine,Shanghai 201203，China；2.Acupuncture and Anesthesia Research Institute,Shuguang Hospital Affiliated with Shanghai University of Traditional Chinese Medicine,Shanghai 201203，China；3.Malaysia Traditional Chinese Medicine Center,Johor Bahru 81200，Malaysia）
关键词:
肝叶切除术; 电针; 足三里; 抗炎细胞因子; 淋巴细胞亚群; 术后感染
Keywords:
Partial hepatectomy; Electroacupuncture; Zusanli; Anti-inflammatory cytokines; Lymphocyte subsets; Postoperative infection
分类号:
R 657.3
DOI:
DOI:10.3969/j.issn.1000-7369.2026.01.002
文献标志码:
A
摘要:
目的：探究电针（EA）足三里对肝叶部分切除术后小鼠免疫功能及炎症反应的影响。方法：按计算机生成的随机数字表法将C57BL/6小鼠随机分成空白组、模型组和电针组。模型组和电针组在全身麻醉下制备70%肝叶切除手术动物模型。电针组分别于术前1 d、术后第1、2、3天于双侧足三里（ST36）处行EA治疗。空白组和模型组不予EA治疗。在术后的第4天，各组小鼠取血和脾脏，进行细胞因子（IL-4、IL-10）和淋巴细胞亚群（CD4+T细胞、CD8+T细胞）的测定，以评估EA对术后小鼠免疫功能和炎症指标的影响。其余各组小鼠给予绿脓杆菌滴鼻，6 h后各组随机选取半数的小鼠进行取材（肺），涂板进行细菌培养，并统计菌落数量。剩余各组小鼠在接种后5 d观察各组小鼠的死亡率，以评估小鼠清除继发感染细菌能力。结果：与空白组比较，模型组小鼠在术后第4天血清IL-4和IL-10水平明显上升，脾脏中CD4+T细胞比例明显减少，肺组织菌落数显著增加和感染后生存率显著降低(P<0.05)。与模型组小鼠相比，电针组小鼠血清IL-4和IL-10水平明显下降，脾脏中CD4+T细胞比例明显增加，肺组织菌落数明显减少和感染后生存率显著上升(P<0.05)。结论：EA足三里可以减轻小鼠肝叶部分切除术后的免疫抑制，并通过降低术后的炎症反应来发挥保护作用。
Abstract:
Objective:To investigate the effects of electroacupuncture (EA) at Zusanli (ST36) on immune function and inflammatory response in mice undergoing partial hepatectomy.Methods:C57BL/6 mice were randomly allocated into a blank group,a model group,and an EA group using a computer-generated random number table.The model and EA groups underwent 70% partial hepatectomy under general anaesthesia to establish the surgical trauma model.The EA group received electroacupuncture at bilateral ST36 one day prior to surgery and on postoperative days 1,2 and 3.The blank and model groups did not receive EA treatment.On postoperative day 4,blood and spleen samples were collected from each group to measure cytokine levels (IL-4,IL-10) and lymphocyte subsets (CD4+T cells,CD8+T cells) to assess the impact of EA on postoperative immune function and inflammatory markers.The remaining mice in each group were subjected to Pseudomonas aeruginosa nasal inoculation.Six hours later,half of the mice in each group were randomly selected for lung tissue collection,bacterial culture plating,and colony counting.The survival rates of the remaining mice were monitored for five days post-inoculation to evaluate their ability to clear secondary bacterial infections.Results:Compared with the blank group,the model group exhibited significantly elevated IL-4 and IL-10 levels,a marked reduction in splenic CD4+ T cell proportion,a significant increase in lung bacterial colonies,and a notably lower post-infection survival rate on postoperative day 4 (P<0.05).In contrast,the EA group demonstrated significantly lower serum anti-inflammatory cytokine (IL-4,IL-10) levels,an increased proportion of splenic CD4+T cells,fewer lung bacterial colonies,and improved post-infection survival rates compared to the model group (P<0.05).Conclusion:Electroacupuncture at ST36 (Zusanli) alleviated immunosuppression in mice following partial hepatectomy and exerted a protective effect by reducing the postoperative inflammatory response.

参考文献/References:

[1]HOGAN B V,PETER M B,SHENOY H G,et al.Surgery induced immunosuppression[J].Surgeon,2011,9(1):38-43.
[2]关昱,仓静.不同麻醉策略对肿瘤术后免疫抑制、复发及转移的影响[J].中国临床医学,2022,29(3):504-513.
[3]HEW D T,PEARSE R M,O’DWYER M J.Does major surgery induce immune suppression and increase the risk of postoperative infection?[J].Curr Opin Anaesthesiol,2016,29(3):376-383.
[4]PEARSE R M,HARRISON D A,MACDONALD N,et al.Effect of a perioperative,cardiac output-guided hemodynamic therapy algorithm on outcomes following major gastrointestinal surgery:A randomized clinical trial and systematic review[J].JAMA,2014,311(21):2181-2190.
[5]STOECKLEIN V M,OSUKA A,LEDERER J A.Trauma equals danger--damage control by the immune system[J].J Leukoc Biol,2012,92(3):539-551.
[6]袁大为,唐焕峰,王华,等.足三里注射联合腹部穴位贴敷对腹腔镜术后胃肠功能恢复及免疫功能、炎症状态的影响[J].中国中西医结合消化杂志,2024,32(12):1111-1115.
[7]赵季宇,苑功名,公一囡,等.针刺疗法中顾护正气思想探究[J].陕西中医,2025,46(2):229-232.
[8]石焱,王佳,陈灵芝.电针联合心理干预对晚期胃癌化疗患者免疫功能及生活质量的影响[J].实用肿瘤杂志,2020,35(5):459-463.
[9]孙芳园,张涛,雷鸣,等.电针足三里穴和关元穴调节脓毒症大鼠免疫功能实验研究[J].黑龙江科学,2019,10(24):64-67.
[10]郭义,赵雪.实验针灸学[M].5版.北京:中国中医药出版社,2016:52-54.
[11]张强,王海波,丁宏胜,等.CD3+和CD4+T水平可预测老年脓毒症患者病情危重及不良预后[J].内科急危重症杂志,2025,31(3):252-254,265.
[12]薛军英,王继超,张建华,等.CD4+/CD8+、改良临床肺部感染评分、营养风险筛查评分与食管癌胸腔镜术后肺部感染关系及意义[J].安徽医药,2024,28(4):773-777.
[13]LONGBOTTOM E R,TORRANCE H D,OWEN H C,et al.Features of postoperative immune suppression are reversible with interferon gamma and independent of interleukin-6 pathways[J].Ann Surg，2016,264(2):370-377.
[14]李杰,文颖娟,王强,等.“行正气”理念视域下针灸调节免疫治疗亚健康状态的思考[J].现代中医药,2025,45(2):21-25.
[15]杨虎,符兆英,张正祥,等.神经内分泌和穴位刺激调节免疫及炎症机制研究进展[J].陕西中医,2022,43(7):982-985.
[16]LI G,LI S,AN L,et al.Electroacupuncture alleviates intraoperative immunosuppression in patients undergoing supratentorial craniotomy[J].Acupunct Med,2013,31(1):51-56.
[17]周伊人,王珂,雍玥,等.免疫紊乱在脓毒症中的作用及针刺抗脓毒症的研究进展[J].上海针灸杂志,2019,38(6):692-698.
[18]HUANG Z,HU Z,OUYANG J,et al.Electroacupuncture regulates the DREAM/NF-κB signalling pathway and ameliorates cyclophosphamide-induced immunosuppression in mice[J].Acupunct Med,2019,37(5):292-300.
[19]代巧妹,王美峤,颜培宇,等.针刺“足三里”对免疫抑制大鼠的作用及穴区CGRP表达影响的研究[J].针灸临床杂志,2018,34(5):49-52.
[20]田嘉禾,万红叶,柳申滨.针刺效应与神经免疫调控——“治病求本”的突破[J].针刺研究,2025,50(5):538-552.
[21]HOTCHKISS R S,MONNERET G,PAYEN D.Immunosuppression in sepsis:A novel understanding of the disorder and a new therapeutic approach[J].Lancet Infect Dis,2013,13:260-268.
[22]IWASZKO M,BIALY S,BOGUNIA-KUBIK K.Significance of interleukin(IL)-4 and IL-13 in inflammatory arthritis[J].Cells,2021,10(11):3000.
[23]OUYANG W,RUTZ S,CRELLIN N K,et al.Regulation and functions of the IL-10 family of cytokines in inflammation and disease[J].Annu Rev Immunol,2010,29:71-109.
[24]NEUMANN C,SCHEFFOLD A,RUTZ S.Functions and regulation of T cell-derived interleukin-10[J].Semin Immunol,2019,44:101344.
[25]BEDKE T,PRETSCH L,KARAKHANOVA S,et al.Endothelial cells augment the suppressive function of CD4+ CD25+ Foxp3+ regulatory T cells:Involvement of programmed death-1 and IL-10[J].J Immunol,2010,184(10):5562-5570.
[26]张建杏,王研,郭远波,等.电针足三里穴对大鼠手术后T淋巴细胞免疫功能的影响[J].南方医科大学学报,2018,38(11):1384-1388.
[27]孙芳园,耿欢,卢明,等.电针通过Toll样受体信号通路调控细胞因子减轻脓毒症免疫抑制的作用及其机制[J].针灸临床志,2022,38(4):61-66.

相似文献/References:

[1]李佩佩,张勇,薛志欣,等.电针对苏丹地区膝骨性关节炎疼痛的疗效观察[J].陕西中医,2017,(06):806.
[2]李牧洋,王婷婷,陈 波△,等.电针足三里环跳穴对不同状态大鼠血清外泌体表达的影响*[J].陕西中医,2019,(2):139.
　LI Muyang,WANG Tingting,CHEN Bo,et al.The effect of electroacupuncture at ZusanliHuantiao acupoints on the expression of serum exosomes of rats in different states[J].,2019,(1):139.
[3]刘 娟,田 磊,赵 杨,等.电针对慢性应激抑郁模型大鼠脑组织内大麻素水解酶的影响[J].陕西中医,2019,(5):553.
[4]魏科祥,黄聪丽,陈小平,等.电针百会穴对创伤后应激大鼠睡眠时相的影响及作用机制研究*[J].陕西中医,2019,(10):1333.
　WEI Kexiang,HUANG Congli,CHEN Xiaoping,et al.Effects of electrical acupuncturing in Baihui acupoint on sleep phases of posttraumatic stress disorder rats and the mechanisms[J].,2019,(1):1333.
[5]吴成举,英锡相,陈 靖,等.针刺疗法对帕金森病大鼠黑质神经元凋亡蛋白bcl2、bax的影响*[J].陕西中医,2020,(1):8.
　WU Chengju,YING Xixiang,CHEN Jing,et al.Effect of acupuncture on apoptosis protein bcl2 and bax 〖JZ〗in substantia nigra neurons of Parkinson’s disease model rats[J].,2020,(1):8.
[6]周 游.电针联合康复训练治疗小儿偏瘫临床研究[J].陕西中医,2020,(2):248.
　ZHOU You..Unified Electro-acupuncture and comprehensive rehabilitation training on children with hemiplegia[J].,2020,(1):248.
[7]惠建荣,羊 璞,赵 娴,等.电针结合传统针刺百会、大椎穴对脑缺血模型大鼠GAP-43表达的影响*[J].陕西中医,2020,(7):856.[doi:DOI:10.3969/j.issn.10007369.2020.07.005]
　HUI Jianrong,YANG Pu,ZHAO Xian,et al.Effect of electroacupuncture and acupuncture Baihui(DU 20)and Dazhui(DU 14)on expression of GAP-43 in erebral ischemia model rats[J].,2020,(1):856.[doi:DOI:10.3969/j.issn.10007369.2020.07.005]
[8]吴仁庆.电针联合全身麻醉对腹部手术患者镇痛效果及血清IL-1β、S100β蛋白和NSE水平的影响[J].陕西中医,2021,(1):118.[doi:DOI:10.3969/j.issn.1000-7369.2020.01.033]
[9]李秀娟,肖亚平,周 钰.电针改善小鼠脓毒症后认知功能障碍作用及机制研究[J].陕西中医,2021,(2):139.[doi:DOI:10.3969/j.issn.1000-7369.2020.02.001]
　LI Xiujuan,XIAO Yaping,ZHOU Yu.Effect and mechanism of electroacupuncture on cognitive dysfunction after sepsis in mice[J].,2021,(1):139.[doi:DOI:10.3969/j.issn.1000-7369.2020.02.001]
[10]杨 阳,田丹丹,樊晓慧.电针缓解完全弗氏佐剂诱导的炎性痛及对前扣带皮层脑区HMGB1/TLR4信号通路的调控作用[J].陕西中医,2022,(1):3.[doi:DOI:10.3969/j.issn.1000-7369.2022.01.001]
　YANG Yang,TIAN Dandan,FAN Xiaohui.Electroacupuncture alleviates CFA induced inflammatory pain and regulates HMGB1/TLR4 pathway in ACC brain region[J].,2022,(1):3.[doi:DOI:10.3969/j.issn.1000-7369.2022.01.001]

备注/Memo

备注/Memo:
国家自然科学基金资助项目(82204869)
更新日期/Last Update: 2026-01-08