[1]吴成举,英锡相,陈 靖,等.针刺疗法对帕金森病大鼠黑质神经元凋亡蛋白bcl2、bax的影响*[J].陕西中医杂志,2020,(1):8-11，133.
　WU Chengju,YING Xixiang,CHEN Jing,et al.Effect of acupuncture on apoptosis protein bcl2 and bax 〖JZ〗in substantia nigra neurons of Parkinson’s disease model rats[J].,2020,(1):8-11，133.
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针刺疗法对帕金森病大鼠黑质神经元凋亡蛋白bcl2、bax的影响*
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《陕西中医》杂志[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2020年1期
页码:
8-11，133
栏目:
基础研究
出版日期:
2020-01-05

文章信息/Info

Title:
Effect of acupuncture on apoptosis protein bcl2 and bax 〖JZ〗in substantia nigra neurons of Parkinson’s disease model rats
文章编号:
DOI:10.3969/j.issn.10007369.2020.01.002
作者:
吴成举; 英锡相; 陈 靖; 谢 鑫; 刘文俊; 张小卿; 吴景东; 马铁明; 马贤德△
辽宁中医药大学(沈阳 110847）
Author(s):
WU Chengju; YING Xixiang; CHEN Jing; et al.
Liaoning University of Traditional Chinese Medicine(Shenyang 110847)
关键词:
帕金森病; 凋亡蛋白; 神经元; 电针; 头针; 大鼠; 鱼藤酮; 关元; 足三里; 太冲
Keywords:
Key words 〖WT5BZ〗〖HT5”SS〗Parkinson’s disease; Apoptotic protein; Neurons;  Electroacupuncture; Scalp; Rats; Rotenone; Guanyuan; Zusanli; Taichong
分类号:
R742.5
文献标志码:
A
摘要:
摘 要 目的:探讨不同方法针灸（“双固一通”电针、头针）“双固一通”法即电针关元、足三里、太冲、风府穴。头针：舞蹈震颤区、运动区、足运感区对帕金森病大鼠脑细胞神经元代谢的影响。方法:除空白对照组外其余注射鱼藤酮（配成1.5 mg/ml油溶液）1.5 mg/(kg·d),连续给药3周造模。随机分为5组(每组n=12)，空白对照组、模型对照组（帕金森动物模型）、电针组（帕金森动物模型+电针治疗）、头针组（帕金森动物模型+头针治疗）、电针+头针组（帕金森动物模型+电针+头针治疗）。HPLC法测定黑质纹状体中DA含量，免疫荧光组化法检测bcl2、bax蛋白表达。结果:模型对照组黑质纹状体中DA含量减少，大鼠中脑黑质bcl2表达显著减少，bax表达显著增加；各针刺组可上调bcl2表达，抑制bax表达。结论:不同方法针灸可以提高PD大鼠黑质多巴胺能神经元合成多巴胺的功能,改善大鼠大鼠的行为学，各针刺组可上调bcl2表达，抑制bax表达。针灸治疗PD为临床提供了理论支持。
Abstract:
Abstract 〖WT5BZ〗〖HT5”SS〗Objective:To explore the different methods of acupuncture “double solid one pass”electric needle,scalp needle“double solid one pass”method is electric needle Guanyuan,Zusanli,Taichong,Fengfu points.Scalp acupuncture:The effects of dance tremor zone,exercise zone and foot movement zone on neuronal metabolism in brain cells of rats with Parkinson’s disease.Methods:In addition to the normal group,the other injections of rotenone (containing 1.5 mg/ml oil solution) 1.5 mg / (kg · d),continuous administration for 3 weeks modeling.randomly divided into 5 groups (n=12 per group),normal blank group,model group (Parkinson animal model),electroacupuncture group (Parkinson animal model + electroacupuncture treatment),scalp group (Parkinson animal model + scalp treatment),electroacupuncture + scalp group (Parkinson animal model + electroacupuncture + scalp treatment).The DA content in the nigrostriatal was determined by HPLC,and the expression of bcl2 and bax protein were detected by immunofluorescence histochemistry.Results:The content of DA in the nigrostriatal of the model group was decreased,the expression of bcl2 in the substantia nigra of rats was significantly decreased,and the expression of bax was significantly increased.The acupuncture group could upregulate the expression of bcl2 and inhibit the expression of bax.Conclusion:Different methods of acupuncture can improve the dopaminergic function of dopaminergic neurons in PD rats and improve the behavior of rats.The acupuncture group can upregulate the expression of bcl2 andinhibit the expression of bax.Acupuncture treatment of PD provides theoretical support for the clinic.〖JP〗

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备注/Memo

备注/Memo:
*辽宁省自然科学基金资助项目（2015020699） 辽宁中医药大学校内课题（2016XJ06）
更新日期/Last Update: 2020-02-17