[1]刘 婷,李 洋,石志红.灯盏花素对非小细胞肺癌A549细胞的作用及机制研究[J].陕西中医杂志,2021,(10):1363-1366.[doi:DOI:10.3969/j.issn.1000-7369.2020.10.009]
　LIU Ting,LI Yang,SHI Zhihong.Effects of breviscapine on proliferation and apoptosis of non-small cell lung cancer A549 cells[J].,2021,(10):1363-1366.[doi:DOI:10.3969/j.issn.1000-7369.2020.10.009]
点击复制

灯盏花素对非小细胞肺癌A549细胞的作用及机制研究
分享到：

《陕西中医》杂志[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年10期
页码:
1363-1366
栏目:
基础研究
出版日期:
2021-10-05

文章信息/Info

Title:
Effects of breviscapine on proliferation and apoptosis of non-small cell lung cancer A549 cells
作者:
刘 婷1; 李 洋2; 石志红2
(1.西安交通大学第一附属医院涉外病房,陕西 西安 710061; 2.西安交通大学第一附属医院呼吸与危重症医学科,陕西 西安 710061)
Author(s):
LIU Ting; LI Yang; SHI Zhihong
(Foreign-related Ward of the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China)
关键词:
非小细胞肺癌;  A549细胞;  灯盏花素;  细胞增殖;  细胞凋亡;  天冬氨酸蛋白水解酶
Keywords:
Non-small cell lung cancer;  A549 cell;  Breviscapine;  Cell proliferation;  Apoptosis;  Cysteinyl aspartate specific proteinase
分类号:
R 734.2
DOI:
DOI:10.3969/j.issn.1000-7369.2020.10.009
文献标志码:
A
摘要:
目的:探讨灯盏花素(BVP)对非小细胞肺癌A549细胞增殖和细胞凋亡的影响。方法:将非小细胞肺癌A549细胞分为对照组、BVP低剂量组(20 μmol/L)、BVP中剂量组(40 μmol/L)、BVP高剂量组(80 μmol/L),使用噻唑蓝(MTT)比色法检测各组细胞12、24、48 h细胞存活率,使用AnnexinV-FITC /PI双染法检测各组细胞24 h细胞凋亡情况,使用蛋白印记法检测各组细胞细胞周期、凋亡相关蛋白表达。结果:BVP低、中、高剂量组A549细胞12、24、48 h时的细胞存活率依次呈降低趋势(P<0.05),作用相同时间时,BVP低、中、高剂量组A549细胞存活率均明显低于对照组(P<0.05),存在剂量效应(P<0.05); BVP低、中、高剂量组24 h细胞凋亡率和Ki67、增殖细胞核抗原(PCNA)、细胞周期蛋白D1(Cyclin D1)、B淋巴细胞瘤-2(Bcl-2)蛋白表达均明显低于对照组(P<0.05),而p21、Bcl-2相关X蛋白(Bax)、天冬氨酸蛋白水解酶-9(Caspase-9)、天冬氨酸蛋白水解酶-3(Caspase-3)蛋白表达量均明显高于对照组(P<0.05),存在剂量效应。结论:BVP可降低非小细胞肺癌A549细胞存活率和促进细胞凋亡,其机制可能与调节细胞增殖、凋亡相关蛋白表达有关。
Abstract:
Objective:To investigate the effects of breviscapine(BVP)on proliferation and apoptosis of non-small cell lung cancer(NSCLC)A549 cells.Methods:NSCLC A549 cells were divided into control group,low-dose BVP group(20 μmol/L),medium-dose BVP group(40 μmol/L)and high-dose BVP group(80 μmol/L).The survival rates of A549 cells at 12,24 and 48 h in each group were detected by methyl thiazolyl tetrazolium(MTT)colorimetry.The apoptosis at 24 h was detected by AnnexinV-FITC /PI double staining method.The cell cycle and expression of apoptosis-related proteins were detected by Western-blot.Results:The survival rates of A549 cells at 12 h,24 h and 48 h were decreased in low-dose,medium-dose and high-dose BVP groups in turn(P<0.05).The survival rate of A549 cells in low-dose,medium-dose and high-dose BVP groups was significantly lower than that in control group(P<0.05),showing dose effect(P<0.05).The apoptosis rate at 24 h,and expression of Ki67,proliferating cell nuclear antigen(PCNA),cyclin D1(Cyclin D1)and B lymphocytoma-2(Bcl-2)in low-dose,medium-dose and high-dose BVP groups were significantly lower than those in control group(P<0.05),while expression levels of p21,Bcl-2 associated X protein(Bax),cysteinyl aspartate specific proteinase-9(Caspase-9)and cysteinyl aspartate specific proteinase-3(Caspase-3)were significantly higher than those in control group(P<0.05),showing dose effect(P<0.05).Conclusion:BVP can reduce survival rate of NSCLC A549 cells and promote apoptosis,which may be related to regulating the expression of proliferation and apoptosis related proteins.

参考文献/References:

[1] 王晓东,景鹏宇,崔 凯,等.术前外周血中性粒细胞淋巴细胞比值对Ⅰ-Ⅲ期非小细胞肺癌患者预后的影响[J].陕西医学杂志,2019,48(12):1612-1615.
[2] 刘桂兰.西妥昔单抗联合NP方案治疗晚期非小细胞肺癌的临床疗效分析[J].医学理论与实践,2017,30(8):1147-1148.
[3] Duma N,Santana-Davila R,Molina JR.Non-small cell lung cancer:Epidemiology,screening,diagnosis,and treatment[J].Mayo Clin Proc,2019,94(8):1623-1640.
[4] 葛文秀,罗 云,谢学恒,等.灯盏花乙素药理作用机制研究进展[J].中国实验方剂学杂志,2020,26(22):193-200.
[5] Liu Y,Wen PH,Zhang XX,et al.Breviscapine ameliorates CCl4 induced liver injury in mice through inhibiting inflammatory apoptotic response and ROS generation[J].Int J Mol Med,2018,42(2):755-768.
[6] Wang Q,Wang Q,Wang SF,et al.Oral Chinese herbal medicine as maintenance treatment after chemotherapy for advanced non-small-cell lung cancer:A systematic review and Meta-analysis[J].Current Oncology,2017,24(4):269-276.
[7] Zeng J,Cai S.Breviscapine suppresses the growth of non-small cell lung cancer by enhancing microRNA-7 expression[J].Journal of Biosciences,2017,42(1):121-129.
[8] 刘俊霞,杨丽丽.非小细胞肺癌合并慢性阻塞性肺疾病老年患者临床特点研究[J].陕西医学杂志,2018,47(4):417-419,429.
[9] 任东峰,孙国芳.六君祛痰解毒汤联合化疗治疗非小细胞肺癌疗效及对患者血管内皮生长因子的影响[J].陕西中医,2020,41(1):63-65.
[10] 刘月芬,刘小红,于明军,等.清金化痰汤治疗非小细胞肺癌临床研究[J].陕西中医,2019,40(2):229-232.
[11] Ke Y,Bao T,Wu X,et al.Scutellarin suppresses migration and invasion of human hepatocellular carcinoma by inhibiting the STAT3/Girdin/Akt activity[J].Biochem Biophys Res Commun,2017,483(1):509-515.
[12] 刘 翼,祝 琳.沉默ECT2对食管癌EC109细胞增殖、细胞周期和裸鼠成瘤能力的影响[J].郑州大学学报:医学版,2020,55(2):203-207.
[13] 张韶凯,罗喜平,李志芳,等.人乳头瘤病毒分型检测在宫颈癌前病变和宫颈癌筛查中的应用价值[J].中华肿瘤杂志,2020,42(3):252-256.
[14] Yang C,Zhang J,Ding M,et al.Ki67 targeted strategies for cancer therapy[J].Clin Transl Oncol,2018,20(5):570-575.
[15] 王范银星,柴 荣,赵家义,等.非小细胞肺癌组织Ki-67与PD-L1表达的相关性及其对患者预后的影响[J].中国肿瘤生物治疗杂志,2019,26(5):536-543.
[16] Tang Y,Cao J,Cai Z,et al.Epigallocatechin gallate induces chemopreventive effects on rats with diethylnitrosamine induced liver cancer via inhibition of cell division cycle 25A[J].Mol Med Rep,2020,22(5):3873-3885.
[17] Guan YB,Yang DR,Nong SJ,et al.Breviscapine(BVP)inhibits prostate cancer progression through damaging DNA by minichromosome maintenance protein-7(MCM-7)modulation[J].Biomed Pharmacother,2017,93(1):103-116.
[18] Azimian H,Dayyani M,Toossi MTB,et al.Bax/Bcl-2 expression ratio in prediction of response to breast cancer radiotherapy[J].Iran J Basic Med Sci,2018,21(3):325-332.
[19] Zhao T,Fu Y,Sun H,et al.Ligustrazine suppresses neuron apoptosis via the Bax/Bcl-2 and Caspase-3 pathway in PC12 cells and in rats with vascular dementia[J].IUBMB Life,2018,70(1):60-70.
[20] Lee H,Kim KC,Hong YM.Changes of Bax,Bcl-2,CCR-2,MCP-1,and TGF-β1 genes in the left ventricle of spontaneously hypertensive rat after losartan treatment[J].Korean J Pediatr,2019,62(3):95-101.
[21] Zhou M,Liu X,Li Z,et al.Caspase-3 regulates the migration,invasion and metastasis of colon cancer cells[J].Int J Cancer,2018,143(4):921-930.
[22] Hngh H,Gh H,Mv J.The Effect of vitamin C on apoptosis and Bax/Bcl-2 proteins ratio in peripheral blood lymphocytes of patients during cardiac interventional procedures[J].J Biomed Phys Eng,2020,10(4):421-432.
[23] 孙 蕾,李伟文,黎 媛,等.IGFBP-4在灯盏花素抑制肺癌A549细胞增殖中的作用[J].温州医科大学学报,2018,48(11):813-817.

相似文献/References:

[1]刘月芬,刘小红,于明军,等.清金化痰汤治疗非小细胞肺癌临床研究[J].陕西中医杂志,2019,(2):229.
　LIU Yuefen,LIU Xiaohong,YU Mingjun,et al.Clinical observation of nonsmall cell lung cancer patients treated with Qingjin Huatan decoction[J].,2019,(10):229.
[2]陈姣红,古红莉,员建中,等.参术扶正抗癌汤联合化疗及三维适形放疗对晚期非小细胞肺癌患者 血清T细胞亚群水平及生存质量的影响*[J].陕西中医杂志,2019,(5):564.
[3]张栓宝,严宁娟△,张燕平.复方斑蝥胶囊对非小细胞肺癌患者围术期血清炎症因子及免疫功能的影响[J].陕西中医杂志,2019,(7):890.
[4]任宝中,李 媛△.益气化瘀方配合化疗治疗晚期非小细胞肺癌临床研究*[J].陕西中医杂志,2019,(8):1036.
　REN Baozhong,LI Yuan..Application effect of TP chemotherapy Plus Yiqi Huayu decoction in advanced Non-small cell lung cancer patients[J].,2019,(10):1036.
[5]任东峰,孙国芳△.六君祛痰解毒汤联合化疗治疗非小细胞肺癌疗效〖JZ〗及对患者血管内皮生长因子的影响[J].陕西中医杂志,2020,(1):63.
[6]肖 凌,王 洋,钱建新,等.生脉饮合沙参麦冬汤联合西药治疗晚期非小细胞肺癌疗效研究[J].陕西中医杂志,2021,(12):1696.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.010]
[7]陈香梅,刘太锋,杨 明,等.复方苦参注射液联合化疗治疗非小细胞肺癌疗效研究[J].陕西中医杂志,2021,(12):1700.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.011]
[8]王理槐,孙银辉,张领兄,等.基于Wnt/β-catenin信号通路探讨血府逐瘀汤在非小细胞肺癌EGFR-TKI获得性耐药中的作用[J].陕西中医杂志,2022,(4):427.[doi:DOI:10.3969/j.issn.1000-7369.2022.04.004]
　WANG Lihuai,SUN Yinhui,ZHANG Lingxiong,et al.Efficacy of Xuefu Zhuyu decoction in EGFR-TKI-Acquired resistance of non-small cell lung carcinoma based on the Wnt/β-catenin signaling pathway[J].,2022,(10):427.[doi:DOI:10.3969/j.issn.1000-7369.2022.04.004]
[9]屈 直,王 芬,李志明,等.二陈汤对Lewis肺癌移植瘤生长及Hedgehog通路蛋白表达的影响[J].陕西中医杂志,2022,(5):555.[doi:DOI:10.3969/j.issn.1000-7369.2022.05.003]
　QU Zhi,WANG Fen,LI Zhiming,et al.Effect of Erchen decoction on the expression of Hedgehog pathway protein in Lewis lung cancer transplanted tumor[J].,2022,(10):555.[doi:DOI:10.3969/j.issn.1000-7369.2022.05.003]
[10]王军徽,肖 萌,马功贤,等.非小细胞肺癌中医药治疗研究进展[J].陕西中医杂志,2023,(11):1663.[doi:DOI:10.3969/j.issn.1000-7369.2023.11.042]
　WANG Junhui,XIAO Meng,MA Gongxian,et al.Research progress in traditional Chinese medicine treatment of non-small cell lung cancer[J].,2023,(10):1663.[doi:DOI:10.3969/j.issn.1000-7369.2023.11.042]

备注/Memo

备注/Memo:
基金项目:陕西省重点研发计划项目(2020KW-053)
更新日期/Last Update: 2021-10-09