[1]陈 宏,张 伟,郭建波,等.地龙提取物通过抑制TGF-β1信号通路发挥抗肺纤维化作用研究[J].陕西中医,2021,(4):427-429.[doi:DOI:10.3969/j.issn.1000-7369.2020.04.005]
　CHEN Hong,ZHANG Wei,GUO Jianbo,et al.Analysis on the antifibrotic effect of earthworm extract by inhibiting TGF-β1 signaling pathway[J].,2021,(4):427-429.[doi:DOI:10.3969/j.issn.1000-7369.2020.04.005]
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地龙提取物通过抑制TGF-β1信号通路发挥抗肺纤维化作用研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年4期
页码:
427-429
栏目:
基础研究
出版日期:
2021-04-05

文章信息/Info

Title:
Analysis on the antifibrotic effect of earthworm extract by inhibiting TGF-β1 signaling pathway
作者:
陈 宏1; 张 伟1; 郭建波1; 苏玉明1; 宋倩男1; 陈 群2; 赵 猛1
(1.黑龙江中医药大学附属第一医院,黑龙江 哈尔滨 150040; 2.哈尔滨市阿城区人民医院,黑龙江 哈尔滨 150300)
Author(s):
CHEN Hong; ZHANG Wei; GUO Jianbo; SU Yuming; SONG Qiannan; CHEN Qun; ZHAO Meng
(The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China)
关键词:
地龙提取物;  TGF-β1信号通路;  上皮细胞;  肺纤维化;  E-钙黏附素
Keywords:
Earthworm extract;  TGF-β1 signaling pathway;  Epithelial cells;  Pulmonary fibrosis;  E-Cadherin
分类号:
R 563.9
DOI:
DOI:10.3969/j.issn.1000-7369.2020.04.005
文献标志码:
A
摘要:
目的:探讨地龙提取物对抑制转化生长因子-β1(TGF-β1)信号通路诱导的肺泡上皮细胞间质转化和肺成纤维细胞活化的影响。方法:MTT法分析地龙提取物对两种细胞增殖的影响。TGF-β1诱导A549肺泡上皮细胞的间质转化和MRC-5肺成纤维细胞的活化; 圆形度定量法分析细胞形态,免疫细胞化学法检测细胞E-钙黏附素(E-cad)和Collagen-Ⅰ蛋白的表达; 双抗体夹心酶联免疫吸附法检测Collagen-Ⅰ和FN水平。结果:对于A549细胞,与正常组比较,TGF-β1组圆形度和E-cad表达均显著降低(P<0.01),Collagen-Ⅰ表达显著升高(P<0.01); 与TGF-β1组比较,地龙组能显著抑制E-cad表达的下调(P<0.05),地龙组能显著抑制Collagen-Ⅰ表达的上调(P<0.05)。对于MRC-5细胞,与正常组比较,TGF-β1组FN、Collagen-Ⅰ的分泌量均显著增加(P<0.01); 与TGF-β1组比较,地龙组的上述两种蛋白的分泌量均显著减少(P<0.05)。结论:地龙提取物抑制TGF-β1诱导的肺泡上皮细胞的间质转化和肺成纤维细胞的活化,提示地龙提取物可能具有抑制肺纤维化的形成和发展的作用。
Abstract:
Objective:To investigate the effect of earthworm extract on inhibition of stromal transformation of alveolar epithelial cells induced by TGF-β1 signaling pathway and activation of lung fibroblasts.Methods:MTT method was used to analyze the effect of earthworm extract on the proliferation of two kinds of cells.TGF-β1 induced mesenchymal transformation of A549 alveolar epithelial cells and activation of mrc-5 lung fibroblasts by TGF-β1.The cell morphology was analyzed by circular quantitative method,and the expression of e-cad and Collagen-Ⅰ protein was detected by immunocytochemistry.Collagen-Ⅰ and FN levels were detected by double antibody sandwich enzyme-linked immunosorbent assay.Results:Compared with the normal group,the expression of Collagen-I and TGF-β1 in A549 cells was significantly decreased(P<0.01),and the expression of Collagen-Ⅰ was significantly increased(P<0.01).Compared with TGF-β1 group 1,earthworm group could significantly inhibit the down-regulation of e-cad expression(P<0.05),while earthworm group could significantly inhibit the up-regulation of Collagen-Ⅰ expression(P<0.05).For mrc-5 cells,compared with the normal group,the secretion levels of FN and Collagen-Ⅰ in TGF- β1 group increased significantly(P<0.01).Compared with TGF-β1 group,the secretion of the two proteins in earthworm group was significantly reduced(P<0.05).Conclusion:Earthworm extract inhibits the stromal transformation of alveolar epithelial cells induced by TGF-β1 and the activation of lung fibroblasts,suggesting that earthworm extract may inhibit the formation and development of pulmonary fibrosis.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81774197,81573863); 中国博士后科学基金第13批特别资助项目(2020T130178); 黑龙江省自然科学基金资助项目(H2018052); 黑龙江省中医药科研项目(ZHY19-004); 黑龙江省博士后科研启动金资助项目(LBH-Q20178)
更新日期/Last Update: 2021-04-09