[1]张容超,吴 涛,刘 奇,等.电针对颅脑损伤大鼠脑组织中PI3K/AKT通路关键蛋白的影响[J].陕西中医,2022,(3):279-282.[doi:DOI:10.3969/j.issn.1000-7369.2022.03.002]
　ZHANG Rongchao,WU Tao,LIU Qi,et al.Effect of electroacupuncture on key proteins of PI3K/AKT signaling pathway in brain tissue of rats with traumatic brain injury[J].,2022,(3):279-282.[doi:DOI:10.3969/j.issn.1000-7369.2022.03.002]
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电针对颅脑损伤大鼠脑组织中PI3K/AKT通路关键蛋白的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年3期
页码:
279-282
栏目:
基础研究
出版日期:
2022-03-05

文章信息/Info

Title:
Effect of electroacupuncture on key proteins of PI3K/AKT signaling pathway in brain tissue of rats with traumatic brain injury
作者:
张容超; 吴 涛; 刘 奇; 李 娜; 郭奎奎; 杜 旭; 王瑞辉
(陕西中医药大学,陕西 咸阳 712046)
Author(s):
ZHANG Rongchao; WU Tao; LIU Qi; LI Na; GUO Kuikui; DU Xu; WANG Ruihui
(Shaanxi University of Chinese Medicine,Xianyang 712046,China)
关键词:
颅脑损伤;  针灸;  电针;  磷脂酰肌醇激酶/蛋白激酶B;  凋亡
Keywords:
Traumatic brain injury;  Acupuncture;  Electroacupuncture;  PI3K/AKT;  Apoptosis
分类号:
R 651.1
DOI:
DOI:10.3969/j.issn.1000-7369.2022.03.002
文献标志码:
A
摘要:
目的:探索电针对颅脑损伤(TBI)大鼠脑组织中介导细胞存活的磷脂酰肌醇激酶/蛋白激酶B(PI3K/AKT)信号通路关键蛋白的影响。方法:选取雄性SD大鼠160只,随机分为空白组、假手术组、模型组、电针组、电针加阻断组、阻断剂组,采用改良Feeney自由落体撞击法制备TBI模型,取大鼠百会、水沟、内关、曲池、足三里、涌泉穴,于造模后24 h介入电针,共14 d。免疫荧光法检测TBI大鼠损伤脑组织PI3K、AKT蛋白的表达量。结果:电针后3、7、14 d,假手术组与空白组PI3K、AKT蛋白表达量比较,差异无统计学意义(均P>0.05)。模型组PI3K、AKT蛋白表达量低于假手术组、高于阻断剂组,差异有统计学意义(均P<0.05)。电针后3 d,电针组PI3K、AKT蛋白表达量与模型组比较,差异无统计学意义(均P>0.05)。电针组在7、14 d时PI3K、AKT表达量均高于模型组,差异有统计学意义(均P<0.05)。结论:电针可促进TBI大鼠损伤脑组织中PI3K、AKT蛋白表达,从而发挥对TBI大鼠脑损伤的康复效应。
Abstract:
Objective:To explore the effect of electroacupuncture on the key proteins of PI3K/AKT signaling pathway that mediate cell survival in brain tissue of rats with traumatic brain injury(TBI).Methods:160 male SD rats were selected and randomly divided into blank group,sham operation group,model group,electroacupuncture group,electroacupuncture combined blocking group and blocking agent group.The modified Feeney free-fall impact method was used to prepare the TBI model.Take the Baihui,Shuigou,Neiguan,Quchi,Zusanli,Yongquan points of the rat,and intervene with electroacupuncture 24 h after modeling for 14 days.The expression levels of PI3K and AKT protein in TBI rats were detected by immunofluorescence method.Results:Comparison of PI3K and AKT protein expression between 3,7,14 d after electroacupuncture,there was no difference between the sham operation group and the blank group(all P>0.05).The model group was lower than the sham operation group and higher than the blocker group, difference statistically significant(all P<0.05).There was no significant difference in PI3K and AKT protein expression between the electroacupuncture group and the model group 3 days after electroacupuncture(all P>0.05).The expression of PI3K and AKT in the electroacupuncture group was higher than those in the model group at 7,14 d,difference statistically significant(all P<0.05).Conclusion:Electroacupuncture can promote the expression of PI3K and AKT protein in the injured brain tissue of TBI rats,thereby exerting the rehabilitation effect on the brain injury of TBI rats.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助面上项目(81674088); 陕西省科技计划项目(2021JQ-735); 陕西省教育厅科研项目(20JK0593)
更新日期/Last Update: 2022-03-09