[1]崔方强,王悦芬,孟 元,等.保肾通络方含药血清对高糖培养足细胞Nephrin、Desmin蛋白表达及细胞凋亡的影响[J].陕西中医,2023,(8):995-999.[doi:DOI:10.3969/j.issn.1000-7369.2023.08.001]
　CUI Fangqiang,WANG Yuefen,MENG Yuan,et al.Effect of drug-containing serum of Baoshen Tongluo recipe on Nephrin and Desmin expression and apoptosis of podocyte in high-glucose environment[J].,2023,(8):995-999.[doi:DOI:10.3969/j.issn.1000-7369.2023.08.001]
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保肾通络方含药血清对高糖培养足细胞Nephrin、Desmin蛋白表达及细胞凋亡的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年8期
页码:
995-999
栏目:
基础研究
出版日期:
2023-08-05

文章信息/Info

Title:
Effect of drug-containing serum of Baoshen Tongluo recipe on Nephrin and Desmin expression and apoptosis of podocyte in high-glucose environment
作者:
崔方强; 王悦芬; 孟 元; 蔡 朕; 江心灿; 赵文景
(首都医科大学附属北京中医医院,北京 100010)
Author(s):
CUI Fangqiang; WANG Yuefen; MENG Yuan; CAI Zhen; JIANG Xincan; ZHAO Wenjing
(Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China)
关键词:
糖尿病肾病;  保肾通络方;  足细胞;  Nephrin蛋白;  Desmin蛋白;  凋亡
Keywords:
Diabetic nephropathy;  Baoshen Tongluo recipe;  Podocyte;  Nephrin protein;  Desmin protein;  Apoptosis
分类号:
R 259
DOI:
DOI:10.3969/j.issn.1000-7369.2023.08.001
文献标志码:
A
摘要:
目的:探讨保肾通络方含药血清对于高糖培养下足细胞肾病蛋白(Nephrin)、结蛋白(Desmin)表达及细胞凋亡的影响。方法:细胞实验分为正常对照组、模型组和保肾通络方低、中、高剂量组。正常对照组足细胞予DMEM低糖培养基培养,模型组和保肾通络方不同剂量组予含有30 mmol/L葡萄糖的高糖培养基培养。保肾通络方不同剂量组足细胞在高糖培养基中分别加入保肾通络方含药血清低、中、高剂量进行干预,正常对照组及模型组加入等剂量空白血清。CCK-8法检测足细胞的细胞活性,免疫荧光及RT-PCR检测足细胞Nephrin、Desmin蛋白及mRNA表达水平,免疫荧光检测足细胞凋亡相关蛋白Caspase-3蛋白表达,Hochest33258染色检测足细胞凋亡情况。结果:与正常对照组比较,模型组足细胞的细胞活性明显降低,Nephrin蛋白表达明显下调,Desmin、Caspase-3蛋白表达明显上调,足细胞凋亡细胞数目明显增加(均P<0.05)。与模型组比较,保肾通络方低、中、高剂量组足细胞的细胞活性明显升高,Nephrin蛋白表达明显上调,Desmin、Caspase-3蛋白表达明显下调,足细胞凋亡数目明显减少(均P<0.05)。结论:保肾通络方可能通过上调足细胞Nephrin蛋白表达,下调Desmin蛋白表达,抑制足细胞凋亡,进而减轻足细胞损伤。
Abstract:
Objective:To observe the effect of drug-containing serum of Baoshen Tongluo recipe on Nephrin and Desmin expression and apoptosis of podocyte in high-glucose environment.Methods:Differentiated mouse podocytes in vitro were divided into normal control group,model group and Baoshen Tongluo recipe low-dose,medium-dose and high-dose groups.The podocyte of normal control group were cultured with DMEM low glucose medium.The podocytes in different dosage groups of Baoshen Tongluo recipe were treated with low,medium and high-doses of Baoshen Tongluo recipe medicated serum in high glucose medium.The normal control group and model group were treated with equal dose of blank serum.Cell viability was detected by CCK-8 assay.Immunofluorescence and RT-PCR were used for detecting Nephrin and Desmin protein and mRNA expression.Immunofluorescence was used for detecting Caspase-3 protein expression.Cell apoptosis was detected by Hochest33258 staining.Results:Compared with normal control group,cell viability of model group was significantly decreased,podocyte apoptosis was significantly increased,Nephrin expression was significantly decreased,Desmin and Caspase-3 expression was significantly increased(all P<0.05).Compared with model group,cell viability of Baoshen Tongluo recipe group was significantly increased,podocyte apoptosis was significantly decreased,Nephrin expression was significantly increased,Desmin and Caspase-3 expression was significantly decreased(all P<0.05).Conclusion:Drug-containing serum of Baoshen Tongluo recipe significantly increased Nephrin expression,decreased Desmin expression of podocyte and ameliorated podocyte apoptosis in high-glucose environment.

参考文献/References:

[1] Lin YC,Chang YH,Yang SY,et al.Update of pathophysiology and management of diabetic kidney disease[J].J Formos Med Assoc,2018,117(8):662-675.
[2] Reidy K,Kang HM,Hostetter T,et al.Molecular mechanisms of diabetic kidney disease[J].J Clin Invest,2014,124(6):2333-2340.
[3] Podgorski P,Konieczny A,Lis L,et al.Glomerular podocytes in diabetic renal disease[J].Adv Clin Exp Med,2019,28(12):1711-1715.
[4] Nagata M.Podocyte injury and its consequences[J].Kidney Int,2016,89(6):1221-1230.
[5] Jiang A,Song A,Zhang C.Modes of podocyte death in diabetic kidney disease:An update[J].J Nephrolm,2022,35(6):1571-1584.
[6] 刘 晶,沈宝华,冉颖卓.益肾固摄活血通络法治疗肾虚血瘀型糖尿病肾病临床疗效[J].陕西中医,2023,44(4):446-450.
[7] Sawaf H,Thomas G,Taliercio JJ,et al.Therapeutic advances in diabetic nephropathy[J].J Clin Med,2022,11(2):378.
[8] Tian X,Bunda P,Ishibe S.Podocyte endocytosis in regulating the glomerular filtration barrier[J].Front Med,2022,10(9):801837.
[9] Liu DW,Zhang JH,Liu FX,et al.Silencing of long noncoding RNA PVT1 inhibits podocyte damage and apoptosis in diabetic nephropathy by upregulating FOXA1[J].Exp Mol Med,2019,51(8):88.
[10] 王 竹,孙万森,吴喜利,等.中药对肾脏足细胞细胞骨架及裂孔隔膜相关蛋白的研究进展[J].陕西中医,2013,34(10):1437-1438.
[11] Li Z,Deng W,Cao A,et al.Huangqi decoction inhibits hyperglycemia-induced podocyte apoptosis by down-regulated Nox4/p53/Bax signaling in vitro and in vivo[J].Am J Transl Res,2019,11(5):3195-3212.
[12] Tung CW,Hsu YC,Shih YH,et al.Glomerular mesangial cell and podocyte injuries in diabetic nephropathy[J].Nephrology,2018,23(4):32-37.
[13] Welsh GI,Saleem MA.Nephrin-signature molecule of the glomerular podocyte?[J].J Pathol,2010,220(3):328-337.
[14] Huang H,You Y,Lin X,et al.Inhibition of TRPC6 signal pathway alleviates podocyte injury induced by TGF-β1[J].Cell Physiol Biochem,2017,41(1):163-172.
[15] Teng B,Schroder P,Muller-Deile J,et al.CIN85 deficiency prevents nephrin endocytosis and proteinuria in diabetes[J].Diabetes,2016,65(12):3667-3679.
[16] Wang C,Hou XX,Rui HL,et al.Artificially cultivated ophiocordyceps sinensis alleviates diabetic nephropathy and its podocyte injury via inhibiting P2X7R expression and NLRP3 inflammasome activation[J].J Diabetes Res,2018,2018:1390418.
[17] 李金红,陶建瓴,李 航,等.足细胞损伤与糖尿病肾病的研究现状[J].中国医学科学院学报,2010,32(5):590-596.
[18] Lian H,Cheng Y,Wu X.TMEM16A exacerbates renal injury by activating P38/JNK signaling pathway to promote podocyte apoptosis in diabetic nephropathy mice[J].Biochem Biophys Res Commun,2017,487(2):201-208.
[19] 徐 进,蒋春波.中药黄芪治肾病“机理”说[J].陕西中医药大学学报,2022,45(2):127-131.
[20] Yao XM,Liu YJ,Wang YM,et al.Astragaloside Ⅳ prevents high glucose-induced podocyte apoptosis via downregulation of TRPC6[J].Mol Med Rep,2016,13(6):5149-5156.
[21] Wang X,Liu J,Tian R,et al.Sanqi oral solution mitigates proteinuria in rat passive heymann nephritis and blocks podocyte apoptosis via Nrf2/HO-1 pathway[J].Front Pharmacol,2021,19(12):727874.
[22] Asadi M,Taghizadeh S,Kaviani E,et al.Caspase-3:Structure,function,and biotechnological aspects[J].Biotechnol Appl Biochem,2022,69(4):1633-1645.
[23] 周媛媛,李中南.李中南治疗糖尿病肾病经验辑要[J].陕西中医药大学学报,2021,44(4):57-60.
[24] 吕仁和,赵进喜,王世东.糖尿病及其并发症的临床研究[J].新中医,2001,33(3):3.
[25] 刘禹辛,尚晓玲,何 泽.何泽基于开阖枢理论谈早中期消渴肾病“毒损肾络”的机理与治疗[J].中医药导报,2022,28(6):141-144.
[26] 孟 元,赵文景,陈 东,等.保肾通络方治疗糖尿病肾脏病(G3期)的前瞻性非随机对照研究[J].世界科学技术-中医药现代化,2020,22(6):1807-1814.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81703989); 北京市自然科学基金资助项目(7222271); 北京市属医院科研培育计划项目(PZ2019013); 首都医科大学科研培育基金资助项目(PYZ22163)
更新日期/Last Update: 2023-08-10