[1]李 羚,苏 虹,粟胜勇,等.麦粒灸对神经根型颈椎病大鼠脊髓及神经根Activin A/ActR Ⅰ/Smad4表达的影响[J].陕西中医,2024,(7):867-871.[doi:DOI:10.3969/j.issn.1000-7369.2024.07.001]
　LI Ling,SU Hong,SU Shengyong,et al.Effects of wheat-grain moxibustion on expression of Activin A/ActR Ⅰ/Smad4 in spinal cord and nerve roots of cervical spondylosis rats[J].,2024,(7):867-871.[doi:DOI:10.3969/j.issn.1000-7369.2024.07.001]
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麦粒灸对神经根型颈椎病大鼠脊髓及神经根Activin A/ActR Ⅰ/Smad4表达的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年7期
页码:
867-871
栏目:
基础研究
出版日期:
2024-07-05

文章信息/Info

Title:
Effects of wheat-grain moxibustion on expression of Activin A/ActR Ⅰ/Smad4 in spinal cord and nerve roots of cervical spondylosis rats
作者:
李 羚1; 苏 虹1; 粟胜勇2; 羊 璞1; 张 熙1; 凌佳霞1
(1.广西中医药大学第一临床医学院,广西 南宁 530200; 2.广西中医药大学第一附属医院针灸科,广西 南宁 530023)
Author(s):
LI Ling; SU Hong; SU Shengyong; YANG Pu; ZHANG Xi; LING Jiaxia
(First Clinical Medical College of Guangxi University of Chinese Medicine,Nanning 530200,China)
关键词:
颈椎病;  颈痛;  麦粒灸;  镇痛机制;  激活素A;  Act I型受体;  Smad4蛋白
Keywords:
Servical spondylosis;  Neck pain;  Wheat-grain moxibustion;  Analgesic mechanism;  Act A;  Act receptor I;  Smad4 protein
分类号:
R 68
DOI:
DOI:10.3969/j.issn.1000-7369.2024.07.001
文献标志码:
A
摘要:
目的:通过观察麦粒灸“大椎”穴对神经根型颈椎病大鼠激活素A(Act A)、Act Ⅰ型受体(ActR Ⅰ)及受体后信号传导蛋白Smad4表达的影响,探讨麦粒灸治疗神经根型颈椎病(CSR)的镇痛机制。方法:将30只SPF级雌雄各半SD大鼠随机分为空白组、模型组、麦粒灸组,每组10只。采用椎管插线法制备CSR模型。模型组及麦粒灸组均于造模后待伤口恢复7 d,第8天开始干预。空白组正常喂养且不做任何干预措施,模型组仅予腹腔注射1 ml的0.9%氯化钠溶液,麦粒灸组在模型组基础上予麦粒灸“大椎”穴,6壮/次,1次/d。连续干预7 d后,观察各组大鼠步态评分,测量机械痛阈值,采用蛋白免疫印迹检测脊髓及神经根组织Act A、ActR Ⅰ及Smad4的蛋白。PCR检测脊髓及神经根组织Act A和ActR Ⅰ的mRNA表达水平,苏木素-伊红染色观察脊髓及神经根组织的形态结构,免疫荧光染色观察Smad4阳性表达水平。结果:与空白组比较,模型组、麦粒灸组大鼠步态评分升高、机械痛阈降低(P<0.05)。与模型组比较,麦粒灸组大鼠脊髓及神经根组织Act A、ActR Ⅰ及其mRNA表达水平均升高(P<0.05)。Western blotting和免疫荧光结果均显示Smad4表达增加; HE染色观察麦粒灸组脊髓形态损伤较模型组有所减轻,灰质区神经元固缩,尼氏小体减少,神经胶质细胞和毛细血管数量比模型组减少。结论:麦粒灸“大椎”穴能改善CSR大鼠疼痛阈值和运动功能,促进神经元损伤后修复,这与激活Activin A/ActR Ⅰ/Smad4信号通路有关。
Abstract:
Objective:To investigate analgesic mechanism of wheat grain moxibustion in the treatment of cervical spondylosis(CSR)by observing effects of wheat grain moxibustion on expression of transmembrane receptor ActRI and its post-receptor signaling protein Smad4 in the Activin A signaling pathway of cervical spondylosis in rats with neurogenic cervical radiculopathy.Methods:Thirty SD ratshalf male and half female of SPF grade were randomly divided into blank control group,model control group and wheat moxibustion group,with 10 rats in each group.The CSR model was prepared by vertebral canal insertion method.Both the model group and wheat moxibustion group were intervened on the 8th day after 7 days of wound recovery after modeling.The blank group were fed normally without any interventions,the model group injected with 1 ml of 0.9% NaCl solution intraperitoneally,and the wheat moxibustion group given wheat moxibustion on the basis of the model group at the point of the “large vertebrae”(6 strokes/times),once a day.After 7 days of continuous intervention,the gait scores of rats in each group were observed,the mechanical pain threshold were measured.Protein expression of Act A,ActR Ⅰ and Smad4 in the spinal cord and nerve root tissues were detected by Western blotting,the expression levels of Act A mRNA and ActR Ⅰ mRNA in the spinal cord and nerve root tissues were detected by PCR,the morphology and structure of the spinal cord and nerve root tissues were observed by HE staining.The level of positive expression of Smad4 was observed by immunofluorescence staining.Results:Compared with the blank group,the gait scores of rats in the model group and the wheat moxibustion group increased and the mechanical pain threshold decreased(P<0.05).The expression levels of Act A,ActR Ⅰ and their mRNAs in the spinal cord and nerve root tissues of rats in the wheat moxibustion group increased compared with those in the model group(P<0.05).Results of Western blotting and immunofluorescence showed an increase in the expression of Smad4.The morphological damage of the spinal cord in the wheat grain moxibustion group was reduced compared with that of the model group.Neurons in the gray matter area were shrunken,Nissen's vesicles reduced,and the number of glial cells and capillaries reduced compared with that of the model group.Conclusion:Wheat grain moxibustion at the “Da Zhui” point improves pain threshold and motor function and promotes neuronal repair after injury in CSR rats,which is related to activation of Activin A/ActR Ⅰ/Smad4 signaling pathway.

参考文献/References:

[1] 邹汉玉,陈翔,赵莉娟,等.牵引治疗神经根型颈椎病机制及应用进展[J].实用医学杂志,2023,39(7):793-797.
[2] 房晓阳,王楠,谢林.从筋骨理论探讨神经根型颈椎病治疗机制[J].陕西中医,2022,43(12):1771-1773.
[3] 马国华,岳学锋.后入路椎间孔切开减压术治疗神经根型颈椎病21例[J].陕西医学杂志,2012,41(11):1526-1527.
[4] 詹红生,冷向阳.中医骨伤学[M].北京:人民卫生出版社,2019:657.
[5] LIANG S,YANG F,ZHANG Y,et al.Changes and clinical correlation of diffusion tensor imaging parameters of compressed spinal cord and nerve root in patients with cervical spondylosis[J].BMC Medical Imaging,2022,22(1):107.
[6] 中华外科杂志编辑部.颈椎病的分型、诊断及非手术治疗专家共识[J].中华外科杂志,2018,56(6):401-402.
[7] CHILDRESS M A,BECKER B A.Nonoperative management of cervical Radiculo-pathy[J].American Family Physician,2016,93(9):74-84.
[8] 曹金,郎雪梅,熊山,等.不同节段颈椎病颈前路手术研究进展[J].陕西医学杂志,2021,50(9):1161-1165.
[9] 赖剑萍,薛爱国.温针灸结合通瘀煎治疗神经根型颈椎病气滞血瘀证疗效研究[J].陕西中医,2023,44(9):1305-1307.
[10] 叶子丰,朱亮亮,常裕绅,等.基于CiteSpace的针灸治疗神经根型颈椎病可视化分析[J].中医药导报,2023,29(6):124-129.
[11] 罗璧玉,符文彬.麦粒灸联合常规针刺治疗颈椎病颈痛的临床疗效分析[J].中国针灸,2018,38(9):931-933.
[12] 王晓彤,王欣欣,董闯,等.中医康复技术操作规范?麦粒灸[J].康复学报,2022,32(2):100-105.
[13] 苏虹,张熙,李竞,等.麦粒灸对坐骨神经损伤大鼠脊髓组织TLR4/MyD88/NF-κB信号通路表达的影响[J].中国中医药信息杂志,2024,31(1):78-83.
[14] 刘童君,赵雅洁,胡洁玲,等.糖代谢异常对血清激活素A水平的影响[J].实用老年医学,2024,38(5):495-499.
[15] 郑少微,展淑琴,张凌峰,等.BDNF与脑缺血严重程度的相关性研究[J].陕西医学杂志,2016,45(2):162-164.
[16] 李宝福,刘柏麟,袁兴,等.辅酶Q10对小鼠脑出血后神经保护作用的实验研究[J].陕西医学杂志,2022,51(11):1337-1340.
[17] 窦夏睿,孙建宁,王威,等.急性期神经根型颈椎病模型的建立[J].北京中医药大学学报,2006,29(5):332-334.
[18] KAWAKAMI M,WEINSTEIN J N,SPRATT K F,et al.Experimental lumbar radiculopathy immunohistochemical and quantitative demonstrations of pain induced by lumbar nerve root irritation of the rat[J].Spine,1994,19(16):1780-1794.
[19] 郭义,赵雪.实验针灸学实验指导[M].北京:中国中医药出版社,2021:217-218.
[20] 路振凯,黄延芹.基于内质网功能探讨“阳化气、阴成形”的科学内涵[J].中华中医药学刊,2022,40(9):149-151.
[21] 王雯雯,赵恒立.基于“阳化气,阴成形”理论治疗痛风性关节炎研究进展[J].陕西中医,2022,43(9):1319-1321.
[22] 李佳辉,刘绮璐,高中洋,等.神经兴奋性对小鼠学习记忆能力的影响[J].陕西医学杂志,2013,42(9):1112-1113.
[23] 董鸿智,王渊.麦粒灸治疗心肾不交型失眠临床研究[J].陕西中医,2015,36(5):605-607.
[24] SOOMRO A,KHAJEHEI M,LI R,et al.A therapeutic target for CKD:Activin A facilitates TGF-β1 profibrotic signaling[J].Cellular & Molecular Biology Letters,2023,28(1):10.
[25] 王若涵,张霄霈,孙佳,等.TGF-β及Smad家族在瘢痕疙瘩形成中作用的研究进展[J].中国细胞生物学学报,2022,44(10):2051-2056.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81960895,82160934); 广西中医药大学桂派杏林拔尖人才项目(2022C017); 广西中医药大学研究生教育创新计划项目(YCBXJ2021018)
更新日期/Last Update: 2024-07-09