[1]廖承成,尹瑞文,张洪卫,等.八桂止痛散对带状疱疹后遗神经痛大鼠脊髓背角组织JNK/p38 MAPK/NF-κB p65通路的影响[J].陕西中医,2025,46(4):450-455.[doi:DOI:10.3969/j.issn.1000-7369.2025.04.004]
　LIAO Chengcheng,YIN Ruiwen,ZHANG Hongwei,et al.Effect of Bagui Zhitong powder on the JNK/p38 MAPK/NF-κB p65 pathway in spinal dorsal horn tissue of rats with postherpetic neuralgia[J].,2025,46(4):450-455.[doi:DOI:10.3969/j.issn.1000-7369.2025.04.004]
点击复制

八桂止痛散对带状疱疹后遗神经痛大鼠脊髓背角组织JNK/p38 MAPK/NF-κB p65通路的影响
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年4期
页码:
450-455
栏目:
基础研究
出版日期:
2025-04-05

文章信息/Info

Title:
Effect of Bagui Zhitong powder on the JNK/p38 MAPK/NF-κB p65 pathway in spinal dorsal horn tissue of rats with postherpetic neuralgia
作者:
廖承成1; 尹瑞文2; 张洪卫1; 张 爽1; 李 霞1; 彭国瑶1; 聂欣怡1
(1.云南中医药大学第一临床医学院皮肤科,云南 昆明 650021; 2.云南中医药大学第一临床医学院脑病科,云南 昆明 650021)
Author(s):
LIAO Chengcheng1; YIN Ruiwen2; ZHANG Hongwei1; ZHANG Shuang1; LI Xia1; PENG Guoyao1; NIE Xinyi1
(1.Department of Dermatology,First Clinical School of Yunnan University of Traditional Chinese Medicine,Kunming 650021,China; 2.Department of Encephalopathy,the First Clinical College of Yunnan University of Traditional Chinese Medicine,Kunming 650021,China)
关键词:
带状疱疹后遗神经痛;  八桂止痛散;  脊髓背角组织;  星形胶质细胞;  炎症;  环氧化酶-2
Keywords:
Postherpetic neuralgia;  Bagui Zhitong powder;  Spinal dorsal horn tissue;  Astrocytes;  Inflammation;  Cyclooxygenase-2
分类号:
R 752.1
DOI:
DOI:10.3969/j.issn.1000-7369.2025.04.004
文献标志码:
A
摘要:
目的:探讨八桂止痛散对带状疱疹后遗神经痛(PHN)大鼠脊髓背角组织JNK/p38 MAPK/NF-κB通路的影响。方法:取SD大鼠腹腔注射树脂毒素(RTX)制备PHN模型,随机分为对照组、模型组及八桂止痛散低、中、高剂量组,每组10只,另取10只SD大鼠作为对照组,以不同剂量八桂止痛散分组干预后检测大鼠长时程自发痛与痛觉过敏症状; 免疫荧光染色检测大鼠脊髓背角组织星形胶质细胞激活标记物GFAP与JNK/p38 MAPK/NF-κB p65通路相关蛋白阳性表达; ELISA检测大鼠脊髓背角组织与血清炎症因子水平; 免疫印迹检测大鼠脊髓背角组织JNK/p38 MAPK/NF-κB p65通路蛋白表达。结果:与对照组比较,模型组大鼠自发缩足反射次数、脊髓背角组织GFAP、p-JNK、p-p38 MAPK及p-NF-κB p65相对阳性表达、血清与脊髓背角组织白细胞介素-6(IL-6)及环氧化酶-2(COX-2)水平、脊髓背角组织p-JNK/JNK、p-p38 MAPK/p38 MAPK、p-NF-κB p65/NF-κB p65显著升高(P<0.05),机械痛阈值、热痛阈值降低(P<0.05); 低、中、高剂量八桂止痛散均可减轻PHN大鼠上述各病理指标变化(P<0.05),且呈剂量依赖性(P<0.05)。结论:八桂止痛散可抑制PHN大鼠脊髓背角组织中星形胶质细胞激活,进而抑制炎症反应,减轻大鼠自发痛与痛觉过敏症状,其机制可能与抑制JNK/p38 MAPK/NF-κB p65信号通路激活相关。
Abstract:
Objective:To investigate the effect of Bagui Zhitong powder on the JNK/MAPK/NF-κB pathway in the spinal dorsal horn tissue of rats with postherpetic neuralgia(PHN).Methods:SD rats were applied to prepare a PHN model by intraperitoneal injection of resin toxin(RTX).They were randomly separated into a control group,a model group,a low-dose Bagui Zhitong powder group,a medium dose Bagui Zhitong powder group,and a high-dose Bagui Zhitong powder group,with 10 rats in each group,another 10 SD rats as the control group,after intervention treatment with different doses of Bagui Zhitong powder,long-term spontaneous pain and hyperalgesia symptoms in rats were detected.The positive expression of GFAP activation markers and JNK/p38 MAPK/NF-κB p65 pathway related proteins in the spinal dorsal horn tissue of rats were detected by immunofluorescence staining.The levels of inflammatory factors in the spinal dorsal horn tissue and serum of rats were detected by ELISA.The expression of JNK/p38 MAPK/NF-κB p65 pathway related proteins in the spinal dorsal horn tissues of rats were detected by immunoblotting.Results:Compared with the control group,the number of spontaneous foot contraction reflexes,relative positive expression of GFAP,p-JNK,p-p38 MAPK,and p-NF-κB p65 in the spinal dorsal horn tissue,the levels of interleukin-6(IL-6)and cyclooxygenase-2(COX-2)in serum and spinal dorsal horn tissue,and p-JNK/JNK,p-p38 MAPK/p38 MAPK,p-NF-κB p65/NF-κB p65 in the spinal dorsal horn tissue of rats in the model group were obviously increased(P<0.05),the mechanical pain threshold and thermal pain threshold were obviously reduced(P<0.05).Low,medium,and high-doses of Bagui Zhitong powder can all alleviate the changes in the above pathological indicators in PHN rats(P<0.05),and were dose-dependent(P<0.05).Conclusion:Bagui Zhitong powder can inhibit the activation of astrocytes in the spinal dorsal horn tissue of PHN rats,thereby reducing the level of inflammatory response,alleviating spontaneous pain and hyperalgesia symptoms in rats which may be achieved by blocking the activation of the JNK/p38 MAPK/NF-κB p65 signaling pathway.

参考文献/References:

[1] 张辰,郭姗姗,谢川,等.梅花针联合普瑞巴林治疗带状疱疹后遗神经痛疗效研究[J].陕西中医,2021,42(3):376-378,382.
[2] BLUMSTEIN A J,FAYNE R,ESHAQ M.Persistent ‘postherpetic neuralgia' and well-demarcated plaque[J].J Fam Pract,2023,72(7):317-319.
[3] CAO X,SHEN Z,WANG X,et al.A meta-analysis of randomized controlled trials comparing the efficacy and safety of pregabalin and gabapentin in the treatment of postherpetic neuralgia[J].Pain Ther,2023,12(1):1-18.
[4] CHEN Y C,FIGLIOZZI R W,HSIA S V.Pilot analyses of interferon subtype expression profiles in patients with herpes zoster or postherpetic neuralgia[J].Viral Immunol,2021,34(7):437-447.
[5] MA L,LI J,ZHOU J,et al.Intravenous lidocaine alleviates postherpetic neuralgia in rats via regulation of neuroinflammation of microglia and astrocytes[J].iScience,2021,4(2):102108-102131.
[6] 成洁,廖承成.八桂止痛膏治疗带状疱疹后遗神经痛的网络药理学研究进展[J].现代临床医学,2022,48(6):452-454.
[7] 李何安安.消炎止痛散治疗带状疱疹疗效观察及镇痛抗炎作用研究[D].昆明:云南中医药大学,2019.
[8] VISHWAKARMA S,SINGH S,SINGH T G.Pharmacological modulation of cytokines correlating neuroinflammatory cascades in epileptogenesis[J].Mol Biol Rep,2022,49(2):1437-1452.
[9] LI X,SHI H,ZHANG D,et al.Paeonol alleviates neuropathic pain by modulating microglial M1 and M2 polarization via the RhoA/p38MAPK signaling pathway[J].CNS Neurosci Ther,2023,29(9):2666-2679.
[10] KHAN A,SHAL B,KHAN A U,et al.Suppression of MAPK/NF-κB and activation of Nrf2 signaling by ajugarin-I in EAE model of multiple sclerosis[J].Phytother Res,2023,37(6):2326-2343.
[11] GUO H M,ZHANG Y,ZHANG Y,et al.Spinal ninjurin2 contributes to the neuropathic pain via NF-κB-mediated neuroinflammation in the spared sciatic nerve injury rats[J].Int Immunopharmacol,2021,99(1):107918-107925.
[12] 常成,宋燕.甘草酸铵对带状疱疹后遗神经痛大鼠GLP-1R/IL-10/β-内啡肽通路及脊髓背角小胶质细胞活化的影响[J].免疫学杂志,2022,38(1):81-87.
[13] 赵丽娟,张艳菊,张诚昊,等.八桂止痛膏对小鼠镇痛抗炎作用效果实验研究[J].中国临床新医学,2022,15(1):74-77.
[14] TANG J,ZHANG Y,LIU C,et al.Therapeutic strategies for postherpetic neuralgia:Mechanisms,treatments,and perspectives[J].Curr Pain Headache Rep,2023,27(9):307-319.
[15] 朱梅,张玉珍,刘影.铺棉灸联合刺络拔罐治疗带状疱疹后遗神经痛临床研究[J].陕西中医,2023,44(3):375-378.
[16] SOJKA P,WLASZCZUK A,OLAKOWSKA E.Potential application of maraviroc in the therapy of neuropathic pain[J].Pol Merkur Lekarski,2021,49(293):379-381.
[17] KONG C,DU J,BU H,et al.LncRNA KCNA2-AS regulates spinal astrocyte activation through STAT3 to affect postherpetic neuralgia[J].Mol Med,2020,26(1):113-124.
[18] NIEMEYER C S,HARLANDER-LOCKE M,BUBAK A N,et al.Trigeminal postherpetic neuralgia:From pathophysiology to treatment[J].Curr Pain Headache Rep,2024,28(4):295-306.
[19] GONIMA V E,MENDOZA W A S,SARMIENTO D A,et al.Analgesic treatment approach for postherpetic neuralgia:A narrative review[J].J Pain Palliat Care Pharmacother,2023,37(2):184-193.
[20] 陈丽娟.消炎止痛膏治疗带状疱疹后神经痛的临床观察及镇痛研究[D].昆明:云南中医药大学,2019.
[21] 李雪,赵婧含,吴文轩,等.桂枝的化学成分和药理作用研究进展[J].中医药学报,2023,51(5):111-114.
[22] 杨伟宁,姚锐,王文升.大黄素对术后肠梗阻大鼠胃肠动力、炎症和氧化应激反应的影响及机制研究[J].陕西医学杂志,2023,52(3):252-256.
[23] 吴文婷,李敏,蒲文娟.白藜芦醇对慢性阻塞性肺疾病大鼠p38丝裂原活化蛋白激酶/核因子-κB信号通路的影响[J].陕西医学杂志,2023,52(11):1489-1492.
[24] GUO L,LI Y,LI W,et al.Shikonin ameliorates oxidative stress and neuroinflammation via the Akt/ERK/JNK/NF-κB signalling pathways in a model of Parkinson's disease[J].Clin Exp Pharmacol Physiol,2022,49(11):1221-1231.
[25] OH S H,KIM S W,KIM D J,et al.Sec-O-glucosylhamaudol mitigates inflammatory processes and autophagy via p38/JNK MAPK signaling in a rat neuropathic pain model[J].Korean J Pain,2021,34(4):405-416.
[26] LI M,LI Z,MA X,et al.Huangqi Guizhi Wuwu decoction can prevent and treat oxaliplatin-induced neuropathic pain by TNF-α/IL-1β/IL-6/MAPK/NF-κB pathway[J].Aging,2022,14(12):5013-5022.

相似文献/References:

[1]谭 强,高 琳,肖月园,等.基于六经辨证运用经方治疗带状疱疹后遗神经痛临床研究*[J].陕西中医,2019,(8):1053.
[2]樵书宏,寇鹏涛,郭娟莉,等.桃红四物汤联合普瑞巴林治疗带状疱疹后遗神经痛疗效及机制研究*[J].陕西中医,2019,(9):1251.
　QIAO Shuhong,KOU Pengtao,GUO Juanli,et al.Efficacy and mechanism of Taohong Siwu decoction combined with pregabalin in the treatment of postherpetic neuralgia[J].,2019,(4):1251.
[3]祝 波,刘 毅△.四虫益气养血汤联合普瑞巴林治疗带状疱疹后遗神经痛临床研究[J].陕西中医,2019,(9):1255.
[4]朱 梅,张玉珍,刘 影.铺棉灸联合刺络拔罐治疗带状疱疹后遗神经痛临床研究[J].陕西中医,2023,(3):375.[doi:DOI:10.3969/j.issn.1000-7369.2023.03.025]
　ZHU Mei,ZHANG Yuzhen,LIU Ying.Clinical study on cotton-covered moxibustion combined with blood-pricking and cupping therapy on postherpetic neuralgia[J].,2023,(4):375.[doi:DOI:10.3969/j.issn.1000-7369.2023.03.025]
[5]廖承成,尹瑞文,张洪卫,等.基于JNK/p38 MAPK/NF-κB信号通路探究八桂止痛散对脂多糖诱导脊髓星形胶质细胞凋亡的影响[J].陕西中医,2024,(4):457.[doi:DOI:10.3969/j.issn.1000-7369.2024.04.005]
　LIAO Chengcheng,YIN Ruiwen,ZHANG Hongwei,et al.The impact of Bagui Zhitong powder on LPS-induced apoptosis of spinal cord astrocytes based on the JNK/p38 MAPK/NF-κB signaling pathway[J].,2024,(4):457.[doi:DOI:10.3969/j.issn.1000-7369.2024.04.005]
[6]周丽芳,王 慈,吴吉婷,等.刺络疗法治疗带状疱疹后遗神经痛临床研究[J].陕西中医,2024,(10):1424.[doi:DOI:10.3969/j.issn.1000-7369.2024.10.029]

备注/Memo

备注/Memo:
[基金项目]云南省科技厅科技计划项目(202001AZ070001-053); 云南省教育厅科学研究基金资助项目(2023Y0488); 云南省冯素英专家工作站建设项目(202105AF150035); 云南省中医医院卓越人才项目[云中附党字(2021)5号]
更新日期/Last Update: 2025-04-08