[1]廖承成,尹瑞文,张洪卫,等.基于JNK/p38 MAPK/NF-κB信号通路探究八桂止痛散对脂多糖诱导脊髓星形胶质细胞凋亡的影响[J].陕西中医杂志,2024,(4):457-462.[doi:DOI:10.3969/j.issn.1000-7369.2024.04.005]
　LIAO Chengcheng,YIN Ruiwen,ZHANG Hongwei,et al.The impact of Bagui Zhitong powder on LPS-induced apoptosis of spinal cord astrocytes based on the JNK/p38 MAPK/NF-κB signaling pathway[J].,2024,(4):457-462.[doi:DOI:10.3969/j.issn.1000-7369.2024.04.005]
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基于JNK/p38 MAPK/NF-κB信号通路探究八桂止痛散对脂多糖诱导脊髓星形胶质细胞凋亡的影响
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《陕西中医》杂志[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年4期
页码:
457-462
栏目:
基础研究
出版日期:
2024-04-05

文章信息/Info

Title:
The impact of Bagui Zhitong powder on LPS-induced apoptosis of spinal cord astrocytes based on the JNK/p38 MAPK/NF-κB signaling pathway
作者:
廖承成1; 尹瑞文2; 张洪卫1; 张 爽1; 李 霞1; 彭国瑶1; 聂欣怡1
(1.云南中医药大学第一临床医学院皮肤科,云南 昆明 650021; 2.云南中医药大学第一临床医学院脑病科,云南 昆明 650021)
Author(s):
LIAO Chengcheng; YIN Ruiwen; ZHANG Hongwei; ZHANG Shuang; LI Xia; PENG Guoyao; NIE Xinyi
(Department of Dermatology,the First Clinical Medical College of Yunnan University of Chinese Medicine,Kunming 650021,China)
关键词:
神经病理性疼痛;  八桂止痛散;  脂多糖;  脊髓星形胶质细胞;  凋亡;  神经炎症
Keywords:
Neuropathic pain;  Bagui Zhitong powder;  LPS;  Spinal cord astrocytes;  Apoptosis;  Neuroinflammation
分类号:
R 285.5
DOI:
DOI:10.3969/j.issn.1000-7369.2024.04.005
文献标志码:
A
摘要:
目的:基于JNK/p38 MAPK/NF-κB信号通路探究八桂止痛散对脂多糖(LPS)诱导的脊髓星形胶质细胞凋亡的影响。方法:从SD乳鼠脊髓中分离星形胶质细胞并鉴定。CCK-8法检测不同浓度八桂止痛散(2.5、5、10、20、40、80 mg/ml)对细胞存活的影响; 随后分别设置对照组、LPS组、LPS+药物-L组、LPS+药物-M组、LPS+药物-H组、LPS+药物-H+Anisomycin组。CCK-8法、流式细胞术检测细胞存活、凋亡; ELISA法检测细胞中白介素(IL)-1β、IL-6、肿瘤坏死因子-α(TNF-α)水平; qRT-PCR检测细胞中JNK、p38 MAPK、NF-κB p65 mRNA表达水平; Western blot检测JNK/p38 MAPK/NF-κB通路相关蛋白表达水平。结果:与对照组相比,LPS组细胞存活率显著降低,细胞凋亡率、IL-1β、IL-6、TNF-α水平、JNK mRNA、p38 MAPK mRNA、NF-κB p65 mRNA表达、p-JNK/JNK、p-p38 MAPK/p38 MAPK、p-NF-κB p65/NF-κB p65表达显著增加(均P<0.05); 与LPS组相比,LPS+药物-L组、LPS+药物-M组、LPS+药物-H组细胞存活率显著增加,细胞凋亡率、IL-1β、IL-6、TNF-α水平、JNK mRNA、p38 MAPK mRNA、NF-κB p65 mRNA表达、p-JNK/JNK、p-p38 MAPK/p38 MAPK、p-NF-κB p65/NF-κB p65表达显著降低,具有剂量依赖性(均P<0.05); 与LPS+药物-H组相比,LPS+药物-H+Anisomycin组细胞存活率显著降低,细胞凋亡率、IL-1β、IL-6、TNF-α水平、JNK mRNA、p38 MAPK mRNA、NF-κB p65 mRNA表达、p-JNK/JNK、p-p38 MAPK/p38 MAPK、p-NF-κB p65/NF-κB p65表达显著增加(均P<0.05)。结论:八桂止痛散可抑制LPS诱导的脊髓星形胶质细胞凋亡,其机制可能与抑制JNK/p38 MAPK/NF-κB信号通路有关。
Abstract:
Objective:To explore the impact of Bagui Zhitong powder on LPS-induced apoptosis of spinal cord astrocytes based on the JNK/p38 MAPK/NF-κB signaling pathway.Methods:Astrocytes were isolated and identified from the spinal cord of SD suckling rats.The effects of different concentrations of Bagui Zhitong powder(2.5,5,10,20,40,80 mg/ml)on cell survival were detected by CCK-8 method.Subsequently,the control group,LPS group,LPS+drug low dose group,LPS+drug medium dose group,LPS+drug high dose group and LPS+drug high dose+Anisomycin group were set,respectively.CCK-8 and flow cytometry were applied to detect cell survival and apoptosis.The levels of interleukin-1β,IL-6 and tumor necrosis factor-α(TNF-α)were detected by ELISA.qRT-PCR was applied to detect the expression levels of JNK mRNA,p38 MAPK mRNA and NF-κB p65 mRNA in cells.Western blot was applied to detect the expression levels of JNK/p38 MAPK/NF-κB pathway related proteins.Results:Compared with the control group,the cell survival rate in the LPS group was obviously reduced,the apoptosis rate,IL-1β,IL-6,TNF-α levels,JNK mRNA,p38 MAPK mRNA,NF-κB p65 mRNA expression,p-JNK/JNK,p-p38 MAPK/p38 MAPK,p-NF-κB p65/NF-κB p65 expression were obviously increased(all P<0.05).Compared with the LPS group,the cell survival rate in the the LPS+drug L group,LPS+drug M group,and LPS+drug H group was obviously increased,the apoptosis rate,IL-1β,IL-6,TNF-α levels,JNK mRNA,p38 MAPK mRNA,NF-κB p65 mRNA expression,p-JNK/JNK,p-p38 MAPK/p38 MAPK,p-NF-κB p65/NF-κB p65 expression were obviously reduced,in a dose dependent manner(all P<0.05).Compared with the LPS+drug H group,the cell survival rate in the LPS+drug H+Anisomycin group was obviously reduced,the apoptosis rate,IL-1β,IL-6,TNF-α levels,JNK mRNA,p38 MAPK mRNA,NF-κB p65 mRNA expression,p-JNK/JNK,p-p38 MAPK/p38 MAPK,p-NF-κB p65/NF-κB p65 expression were obviously increased(all P<0.05).Conclusion:Bagui Zhitong powder can inhibit LPS-induced apoptosis of spinal astrocytes,which may be related to the inhibition of JNK/p38 MAPK/NF-κB signaling pathway.

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[1]钟青华,杨 松,孟 灵,等.电针颈夹脊穴对神经病理性疼痛大鼠脊髓背角谷氨酸受体及趋化因子受体表达水平的影响[J].陕西中医杂志,2022,(5):564.[doi:DOI:10.3969/j.issn.1000-7369.2022.05.005]
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备注/Memo

备注/Memo:
基金项目:云南省科技厅科技计划项目(202001AZ070001-053); 云南省教育厅科学研究基金资助项目(2023Y0488); 云南省冯素英专家工作站建设项目(202105AF150035); 云南省中医医院卓越人才项目[云中附党字(2021)5号]
更新日期/Last Update: 2024-04-10