[1]邓祥丽,陈丽娟,邵 梅,等.益肺汤调控TGF-β1/Smad2通路抗肺纤维化机制研究[J].陕西中医杂志,2024,(4):435-439.[doi:DOI:10.3969/j.issn.1000-7369.2024.04.001]
　DENG Xiangli,CHEN Lijuan,SHAO Mei,et al.Mechanism of Yifei decoction regulating TGF-β1/Smad2 signaling pathway against pulmonary fibrosis[J].,2024,(4):435-439.[doi:DOI:10.3969/j.issn.1000-7369.2024.04.001]
点击复制

益肺汤调控TGF-β1/Smad2通路抗肺纤维化机制研究
分享到：

《陕西中医》杂志[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年4期
页码:
435-439
栏目:
基础研究
出版日期:
2024-04-05

文章信息/Info

Title:
Mechanism of Yifei decoction regulating TGF-β1/Smad2 signaling pathway against pulmonary fibrosis
作者:
邓祥丽1; 陈丽娟2; 邵 梅1; 吴 梅1; 杨 梅1; 吴海茵1; 马信文1; 黄 芬1
(1.云南中医药大学第一临床医学院,云南 昆明 650051; 2.云南中医药大学第一附属医院,云南 昆明 650021)
Author(s):
DENG Xiangli; CHEN Lijuan; SHAO Mei; WU Mei; YANG Mei; WU Haiyin; MA Xinwen; HUANG Fen
(The First Clinical Medical College of Yunnan University of Traditional Chinese Medicine,Kunming 650051,China)
关键词:
肺纤维化;  益肺汤;  TGF-β1/Smad2信号通路;  血管紧张素Ⅱ;  肺成纤维细胞;  α-平滑肌肌动蛋白
Keywords:
Pulmonary fibrosis;  Yifei decoction;  TGF-β1/Smad2 signaling pathway;  Angiotensin Ⅱ;  NIH-3T3;  α-SMA
分类号:
R 563
DOI:
DOI:10.3969/j.issn.1000-7369.2024.04.001
文献标志码:
A
摘要:
目的:研究益肺汤含药血清对血管紧张素Ⅱ(Ang Ⅱ)诱导的肺成纤维细胞(NIH-3T3)的影响,探讨其抑制肺纤维化的作用机制。方法:采用SPF级C57小鼠灌胃益肺汤,1次/d,连续给药3 d制备含药血清。实验分为正常对照组、模型组、模型+正常血清组、模型+含药血清组,给药48 h后0.1%FBS的培养基饥饿处理24 h,100 ng/μl的Ang Ⅱ诱导造模24 h。免疫组化检测α-平滑肌肌动蛋白(α-SMA); 免疫荧光检测Smad2; Western blot检测Ⅰ型胶原蛋白(Col Ⅰ)、α-SMA、纤维连接蛋白(FN)、Smad2; ELISA检测细胞上清转化生长因子-β1(TGF-β1)的表达情况。结果:Western blot和免疫荧光显示,造模后,α-SMA、FN、Col Ⅰ、Smad2的蛋白相对表达显著升高,差异具有统计学意义(均P<0.05)。益肺汤含药血清干预48 h后造模,α-SMA、FN、Smad2、Col Ⅰ的相对量较模型组下降(均P<0.05)。ELISA结果显示,造模后,TGF-β1的相对表达显著升高(P<0.05),益肺汤含药血清干预48 h后,TGF-β1相对量较模型组下降(P<0.05)。结论:益肺汤含药血清对Ang Ⅱ诱导的NIH-3T3有一定影响,可有效减轻肺纤维化,其作用机制可能与调控TGF-β1/Smad2信号通路,降低α-SMA、Col Ⅰ、FN的表达有关。
Abstract:
Objective:To study the effect of serum containing Yifei decoction on NIH-3T3 induced by angiotensin Ⅱ(Ang Ⅱ),and to explore its mechanism of inhibiting pulmonary fibrosis.Methods:SPF C57 mice were given Yifei decoction once a day for 3 days to prepare medicated serum.The experiment was divided into normal control group,model group,model+normal serum group and model+medicated serum group.After 48 hours of administration,0.1%FBS was starved for 24 hours,and 100 ng/μl Ang Ⅱ was used to induce the model for 24 hours.α-smooth muscle actin(α-SMA)was detected by immunohistochemistry.Smad2 was detected by immunofluorescence.Collagen Ⅰ(Col Ⅰ),α-SMA,fibronectin(FN)and Smad2 were detected by Western blot.The expression of transforming growth factor-β1(TGF-β1)in cell supernatant was detected by ELISA.Results:Western blot and immunofluorescence showed that the relative expression of α-SMA,FN,Col Ⅰ and Smad2 increased significantly after modeling,with statistical significance(all P<0.05).After 48 hours of intervention with medicated serum,the relative amounts of α-SMA,FN,Smad2 and Col Ⅰ decreased locally compared with the model group(all P<0.05).The results of ELISA showed that the relative expression of TGF-β1 increased significantly after modeling(P<0.05),the relative amount of TGF-β1 decreased locally compared with the model group after 48 hours of intervention with drug-containing serum(P<0.05).Conclusion:The serum containing Yifei decoction has certain effect on NIH-3T3 induced by Ang Ⅱ,which can effectively alleviate pulmonary fibrosis.This mechanism may be related to the regulation of TGF-β1/Smad2 signaling pathway and the reduction of α-SMA,Col Ⅰ and FN expression.

参考文献/References:

[1] MEYER K C.Pulmonary fibrosis part Ⅰ:Epidemiology pathogenesis and diagnosis[J].Expert Rev Respir Med,2017,11(5):343-359.
[2] 邓永红,王宏坤,刘伟.特发性肺纤维化预后危险因素分析[J].陕西医学杂志,2015,44(9):1149-1151.
[3] KSEIBATI M O,SHARAWY M H,SALEM H A.Corrigendum to “chrysin mitigates bleomycin-induced pulmonary fibrosis in rats through regulating inflammation,oxidative stress,and hypoxia”[J].Int Immunopharmacol,2023,117:109935.
[4] 杨阳,周秋云,宋映容,等.基于openFDA对吡非尼酮和尼达尼布药品不良反应的比较分析[J].药学与临床研究,2022,30(3):263-266.
[5] LODYGA M,HINZ B.TGF-β1-A truly transforming growth factor in fibrosis and immunity[J].Semin Cell Dev Biol,2020,101:123-139.
[6] HU H H,CHEN D Q,WANG Y N,et al.New insights into TGF-β/Smad signaling in tissue fibrosis[J].Chemico-biological Interactions,2018,292:76-83.
[7] DONG J,MA Q.Myofibroblasts and lung fibrosis induced by carbon nanotube exposure[J].Particle and Fibre Toxicology,2016,13(1):60.
[8] RUAN H,LV Z,LIU S,et al.Anlotinib attenuated bleomycin-induced pulmonary fibrosis via the TGF-β1 signalling pathway[J].The Journal of Pharmacy and Pharmacology,2020,72(1):44-55.
[9] WILSON M S,WYNN T A.Pulmonary fibrosis:Pathogenesis,etiology and regulation[J].Mucosal Immunology,2009,2(2):103-121.
[10] 张茜茜,汪铮,安云霞,等.血管紧张素Ⅱ对大鼠肺部纤维化的诱导机制[J].西安交通大学学报(医学版),2019,40(3):352-355.
[11] 李雪,李晗,赵凤莲,等.肾素血管紧张素系统在呼吸系统疾病发生和发展中的研究进展[J].国际老年医学杂志,2022,43(2):232-236.
[12] 张文斌,王杰,王玮,等.健脾益肺汤抑制NF-κB/STAT3信号通路改善哮喘模型大鼠气道炎症及气道重塑作用研究[J].中国中医急症,2019,28(5):806-808.
[13] 于丹,王莹,高原,等.补中益气汤含药血清对TGF-β介导的A549/DDP细胞Snail、E-cadherin表达的影响[J].中国老年学杂志,2023,43(7):1636-1639.
[14] 成文媛,钱之玉,杨丽娜,等.西红花酸在Ang Ⅱ诱导的大鼠肺成纤维细胞中的作用[J].药学与临床研究,2012,20(2):103-108.
[15] LIANG H H,XU C,PAN Z,et al.The antifibrotic effects and mechanisms of microRNA-26a action in idiopathic pulmonary fibrosis[J].Mol Ther,2014,22(6):1122-1133.
[16] 王慧慧,蒙艳丽,杨志敏,等.地龙对肺纤维化小鼠肺组织中纤维化因子TGF-β1及α-SMA表达的影响[J].中国中药杂志,2019,44(24):5473-5478.
[17] 黄健华,白舒琳,刘锐.基于“气血”理论探讨中医治疗肺系疾病之思路[J].陕西中医,2023,44(4):483-486.
[18] 吴文玉,张玺金,王凯,等.肺脾相关理论在肺系疾病中的运用[J].陕西中医,2023,44(1):88-92.
[19] 张召杨,孙增涛,刘南飞,等.肺脾相关理论及临床应用研究[J].陕西中医,2020,41(11):1623-1626.
[20] PHAN T H G,PALIOGIANNIS P,NASRALLAH G K,et al.Emerging cellular and molecular determinants of idiopathic pulmonary fibrosis[J].Cell Mol Life Sci,2021,78(5):2031-2057.
[21] YAN Z,KUI Z,PING Z.Reviews and prospectives of signaling pathway analysis in idiopathic pulmonary fibrosis[J].Autoimmunity Reviews,2014,13(10):1020-1025.
[22] 黄霂晗,韩佳,徐昌君,等.黄芪甲苷对肺纤维化小鼠α-SMA、Ⅰ型和Ⅲ型胶原表达影响[J].辽宁中医药大学学报,2019,21(1):47-50.
[23] 栾智华,任晋宏,薛慧清,等.黄芪糖蛋白对肺纤维化小鼠肺组织中α-SMA表达的影响[J].中华中医药学刊,2020,38(6):197-200.
[24] PARDALI E,SANCHEZ-DUFFHUES G,GOMEZ-PUERTO M C,et al.TGF-β-induced endothelial-mesenchymal transition in fibrotic diseases[J].International Journal of Molecular Sciences,2017,18(10):2157.
[25] WENG D,CHEN J X,LI H H,et al.2-aminopurine suppresses the TGF-β1-induced epithelial-mesenchymal transition and attenuates bleomycin-induced pulmonary fibrosis[J].Cell Death Discovery,2018,4:17.
[26] 付蓓蓓,陆雨琳,陈萌檬,等.TGF-β/Smad信号通路在类风湿性关节炎相关的间质性肺纤维化小鼠中的作用[J].中国药理学通报,2023,39(3):483-488.
[27] WALTON K L,JOHNSON K E,HARRISON C A.Targeting TGF-β mediated SMAD signaling for the prevention of fibrosis[J].Frontiers in Pharmacology,2017,8:461.
[28] 孙林清,周倩文,王发选.转化生长因子β1对肺成纤维细胞中平滑肌肌动蛋白表达的影响[J].宁夏医科大学学报,2019,41(5):461-464.
[29] 张旭辉,刘喜平,孙杰,等.升陷汤对肺纤维化大鼠肺组织α-平滑肌肌动蛋白、肺表面活性蛋白D及TGF-β1/Smads通路蛋白表达的影响[J].中国中医药信息杂志,2021,28(4):63-68.
[30] 冯佳,向阳,夏燕,等.益气养阴方对大鼠肺纤维化的干预作用及对Smad2、Smad7蛋白的影响[J].中国免疫学杂志,2015,31(3):334-338.
[31] 邓玲玲,欧阳博书,魏颖,等.上皮间质转化在特发性肺纤维化及其信号通路中的研究进展[J].复旦学报(医学版),2022,49(4):614-619.
[32] 马定虎,周宗涛,李政,等.ZLY18通过抑制TGF-β1/Smads通路改善血管紧张素Ⅱ诱导的心肌纤维化[J].中国药理学通报,2023,39(2):229-238.

相似文献/References:

[1]胡少丹,仕 丽△,孙 峰,等.助阳补肺除痹颗粒对肺纤维化大鼠肺组织超氧化物歧化酶活性、丙二醛含量影响的研究*[J].陕西中医杂志,2019,(6):683.
　HU Shaodan,SHI Li,SUN Feng,et al.Effect of Zhuyang Bufei Chubi granule on superoxide dismutase activity and malondialdehyde content in lung tissue of rats with pulmonary fibrosis[J].,2019,(4):683.
[2]杭 程,肖 洋,王高雷,等.米烈汉教授基于宗气为本防治肺纤维化经验浅析[J].陕西中医杂志,2021,(9):1282.[doi:DOI:10.3969/j.issn.1000-7369.2020.09.036]
[3]李世霞,张旭辉,李红梅,等.肺纤维化中内质网应激作用机制及中医药防治研究进展[J].陕西中医杂志,2023,(12):1819.[doi:DOI:10.3969/j.issn.1000-7369.2023.12.034]

备注/Memo

备注/Memo:
基金项目:国家自然科学基金地区基金资助项目(82360922); 云南省科技厅科技计划项目-中医联合面上项目[2019FF002(-034)]; 云南中医药大学第一临床医学院研究生科学研究基金资助项目(2022YS12)
更新日期/Last Update: 2024-04-10