[1]李 娜,王瑞辉,郭 婕,等.电针对创伤性颅脑损伤大鼠炎症因子表达及神经功能的影响[J].陕西中医,2021,(10):1358-1362.[doi:DOI:10.3969/j.issn.1000-7369.2020.10.008]
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电针对创伤性颅脑损伤大鼠炎症因子表达及神经功能的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年10期
页码:
1358-1362
栏目:
基础研究
出版日期:
2021-10-05

文章信息/Info

作者:
李 娜王瑞辉郭 婕李 华郭新荣张容超吴 涛
(陕西中医药大学针灸推拿学院,陕西 咸阳 712046)
关键词:
颅脑损伤 电针 炎症因子 神经元 血清炎症因子 大鼠
分类号:
R 651.15
DOI:
DOI:10.3969/j.issn.1000-7369.2020.10.008
文献标志码:
A
摘要:
目的:探讨电针通过抑制颅脑损伤模型大鼠炎症反应,改善神经功能发挥脑保护作用的机制。方法:将66只雄性SD大鼠,随机分为正常对照组、假手术组各12只(其中脑组织含水量测定各6只)、模型对照组、电针组各21只,模型组和电针组再按照手术后1、3、7 d,分成亚组3个,每个亚组为7只; 模型制备采用改良 feeney's 自由落体打击法进行大鼠创伤性颅脑损伤模型制备,干预方法采用电针干预,选取穴位“百会”“水沟”及双侧“内关”“足三里”,在造模后 12 h 对大鼠进行电针干预,电针参数为断续波,频率 2 Hz, 1 次/d, 15 min/次,假手术组仅仅暴露颅骨和钻孔,不进行改良Feeney's自由落体打击,分别干预 1、3、7 d。大鼠神经功能损伤程度的评估采用经过改良的神经功能缺损量表(mNSS)进行评估; 大鼠损伤脑组织含水量采用干湿重法进行检测; 损伤大脑组织中白细胞介素(IL-1β)、肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)蛋白表达采用蛋白质免疫印迹法(WB)检测; 血清炎症因子:IL-1β、TNF-α、IL-6的表达采用酶联免疫吸附法(ELISA)检测。结果:与假手术组相比,模型组大鼠损伤脑组织中蛋白:IL-1β、TNF-α、IL-6的相对表达量明显升高(P<0.05); 血清炎症因子:IL-1β、TNF-α、 IL-6的含量水平显著升高( P<0.05); 大鼠损伤脑组织含水量显著升高(P<0.05); mNSS评分显著升高(P<0.05)。相比于模型组,电针组大鼠损伤脑组织中蛋白:IL-1β、TNF-α、IL-6的相对表达量降低明显(P<0.05); 血清炎症因子:IL-1β、TNF-α、 IL-6的含量水平显著降低(P<0.05); 损伤脑组织含水量降低明显(P<0.05); mNSS评分明显降低(P<0.05)。结论:电针可降低TNF-α、IL-1β、IL-6等炎症因子的含量,能对创伤性颅脑损伤诱发的炎症反应产生抑制作用,减轻大鼠脑组织水肿情况,从而降低对损伤周围脑组织神经元的损害,保护脑神经功能。

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81674088); 陕西省科技计划项目(2021JQ-735)
更新日期/Last Update: 2021-10-09