[1]刘海艳,张春玲,赵 伟,等.基于PINK1/Parkin信号通路探讨丹黄散在糖尿病小鼠溃疡创面愈合中的作用[J].陕西中医,2023,(4):433-436.[doi:DOI:10.3969/j.issn.1000-7369.2023.04.005]
　LIU Haiyan,ZHANG Chunling,ZHAO Wei,et al.Effect of Danhuang powder on ulcer wound healing in diabetic mice based on PINK1/Parkin signaling pathway[J].,2023,(4):433-436.[doi:DOI:10.3969/j.issn.1000-7369.2023.04.005]
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基于PINK1/Parkin信号通路探讨丹黄散在糖尿病小鼠溃疡创面愈合中的作用
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年4期
页码:
433-436
栏目:
基础研究
出版日期:
2023-04-05

文章信息/Info

Title:
Effect of Danhuang powder on ulcer wound healing in diabetic mice based on PINK1/Parkin signaling pathway
作者:
刘海艳1; 张春玲2; 赵 伟2; 邸铁涛2; 陈 露2; 王庚新1; 王艳辉1; 董源源1; 范 燚1
(1.贵州中医药大学,贵州 贵阳 550025; 2.贵州中医药大学第二附属医院,贵州 贵阳 550003)
Author(s):
LIU Haiyan; ZHANG Chunling; ZHAO Wei; DI Tietao; CHEN Lu; WANG Gengxin; WANG Yanhui; DONG Yuanyuan; FAN Yi
(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)
关键词:
糖尿病;  溃疡;  丹黄散;  小鼠;  线粒体自噬;  PINK1/Pakrin信号通路
Keywords:
Diabetic;  Ulcer;  Danhuan powder;  Mice;  Mitochondrial autophagy;  PINK1/Pakrin signaling pathway
分类号:
R 587.2
DOI:
DOI:10.3969/j.issn.1000-7369.2023.04.005
文献标志码:
A
摘要:
目的:基于同源性磷酸酶张力蛋白诱导激酶1(PINK1)/帕金森蛋白(Parkin)信号通路探讨丹黄散在糖尿病小鼠溃疡创面愈合中的作用。方法:选取18只SPF级雄性db/db小鼠,随机分为模型组、生长因子组、丹黄散组,每组6只,另选取6只SPF级雄性db/m小鼠作为空白组,各组小鼠均构建糖尿病溃疡模型。空白组与模型组小鼠以0.9%氯化钠溶液清创并用无菌纱布覆盖创面,生长因子组与丹黄散组小鼠在其基础上分别外敷表皮生长因子凝胶和丹黄散。观察比较各组小鼠溃疡创面愈合情况及PINK1/Parkin信号通路关键蛋白表达水平。结果:模型组第7、14、21天的创面愈合率明显低于丹黄散组、生长因子组(均P<0.05); HE染色和电镜观察创面组织,相较于模型组,生长因子组、丹黄散组创面肉芽组织病理改变明显减轻,大量成纤维细胞增生,致密分布,出现少量新生毛细血管,PINK1、Pakin和LC3-Ⅱ/Ⅰ蛋白表达水平均明显增加(均P<0.05)。结论:丹黄散可促进糖尿病小鼠溃疡创面愈合,可能通过调节PINK1/Pakrin信号通路进而调控线粒体自噬,保护线粒体功能,促进血管新生和肉芽组织生长,加快创面愈合。
Abstract:
Objective:To investigate the effect of Danhuang powder on ulcer wound healing in diabetic mice based on PINK1/Parkin signaling pathway.Methods:Eighteen SPF male db/db mice were randomly divided into model group,growth factor group and Danhuang powder group,6 mice in each group.Another 6 SPF male db/m mice were selected as blank group,diabetic ulcer models were constructed in each group.Blank group and model group were debrideed with 0.9% sodium chloride solution and covered with sterile gauze.Growth factor group and Danhuang powder group were applied to epidermal growth factor gel and Danhuang powder respectively.The ulcer wound healing and the levels of key proteins in PINK1/Parkin signaling pathway were observed and compared in each group.Results:The wound healing rate of model group at day 7,14 and 21 was lower than that of Danhuang powder group,growth factor group(P<0.05).Compared with model group,the pathological changes of granulation tissue in growth factor group and Danhuang powder group were significantly reduced.A large number of fibroblasts proliferated and distributed in a compact way and a few new capillaries appeared.Protein expression levels of PINK1,Pakin and LC3-Ⅱ/Ⅰwere significantly increased(all P<0.05).Conclusion:Danhuang powder can promote ulcer wound healing in diabetic mice,possibly regulate mitochondrial autophagy by regulating PINK1/Pakrin signaling pathway,protect mitochondrial function,promote angiogenesis and granulation tissue growth,and accelerate wound healing.

参考文献/References:

[1] Yang S,Xu M,Meng G,et al.SIRT3 deficiency delays diabetic skin wound healing via oxidative stress and necroptosis enhancement[J].J Cell Mol Med.2020,24(8):4415-4427.
[2] 冯海霞,彭新宇,董 峰.阳和汤加减联合银离子敷料治疗糖尿病足溃疡临床研究[J].陕西中医,2021,42(7):900-902.
[3] 马英丽,姬秋和.清创换药联合康复新液对糖尿病足溃疡的临床疗效观察[J].陕西医学杂志,2016,45(5):636.
[4] 郭园园.AGEs通过诱导巨噬细胞自噬影响糖尿病创面愈合的机制研究[D].上海:上海交通大学,2016.
[5] 王松海,康 超,谢燕华,等.红景天苷通过抑制线粒体自噬通路减轻1-甲基-4-苯基吡啶离子诱导的PC12细胞凋亡实验研究[J].陕西医学杂志,2021,50(6):652-656.
[6] Qu K,Cha H,Ru Y,et al.Buxuhuayu decoction accelerates angiogenesis by activating the PI3K-Akt-eNOS signalling pathway in a streptozotocin-induced diabetic ulcer rat model[J].Journal of Ethnopharmacology,2021,273:113824.
[7] 王 冠,刘 明,栾耀芳,等.四黄洗药汤治疗糖尿病足溃疡疗效观察[J].陕西中医,2017,38(11):1558-1559.
[8] 龚海峰,李 强,郝 治,等.以西红花为主药配伍的油剂治疗严重糖尿病足溃疡临床研究[J].陕西中医,2018,39(7):916-918.
[9] 王安宇,乔艺杰,魏良纲.丹黄散外敷治疗糖尿病足的临床疗效观察[J].贵州医药,2011,35(6):505-507.
[10] 张定安,仲晓宁,张 超,等.丹黄散对糖尿病大鼠创面愈合及新生皮肤中CK14、CK15表达的影响研究[J].现代中西医结合杂志,2020,29(7):702-709.
[11] Ma HJ,Guo ZY,Gai C,et al.Protective effects of buyinqianzheng formula on mitochondrial morphology by PINK1/Parkin pathway in SH-SY5Y cells induced by MPP+ [J].J Tradit Chin Med Sci,2020,7(3):274-282.
[12] Youle RJ,Narendra DP.Mechanisms of mitophagy[J].Nature Reviews Molecular Cell Biology,2011,12(1):9-14.
[13] 汤友静,牛玉娜,王 辉.PINK1/Parkin介导的线粒体自噬分子机制[J].中国细胞生物学学报,2017,39(7):939-946.
[14] Narendra DP,Jin SM,Tanaka A,et al.PINK1 is selectively stabilized on impaired mitochondria to activate Parkin[J].PLoS Biol,2010,8(1):e1000298.
[15] Rub C,Wilkening A,Voos W.Mitochondrial quality control by the PINK1/Parkin system[J].Cell Tissue Res,2017,367(1):111-123.
[16] Tanida I,Ueno T,Kominami E.LC3 and autophagy[J].Methods Mol Biol,2008,445:77-88.
[17] Mizushima N,Komatsu M.Autophagy:Renovation of cells and tissues[J].Cell,2011,147(4):728-741.
[18] Kabeya Y,Mizushima N,Ueno T,et al.LC3,a mammalian homologue of yeast Apg8p,is localized in autophagosome membranes after processing[J].EMBO J,2000,19(21):5720-5728.
[19] 叶 亮,袁 淼,肖文峰,等.脑损伤模型大鼠PINK1/Parkin介导的线粒体自噬作用[J].中国组织工程研究,2020,24(11):1695-1700.
[20] 阚 彤.Sirt3调控FOXO3a/PINK1-Parkin介导的线粒体自噬促进糖尿病性角膜上皮愈合的机制研究[D].福州:福建医科大学,2017.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81960805); 贵州省教育厅创新群体重大研究项目[黔教合KY字(2017)042]
更新日期/Last Update: 2023-04-10