[1]蒋钰涵,王 锐,于钦明.养心汤调控TLR4/NLRP3信号通路治疗抑郁症大鼠机制[J].陕西中医,2025,46(3):306-310.[doi:DOI:10.3969/j.issn.1000-7369.2025.03.004]
　JIANG Yuhan,WANG Rui,YU Qinming.Mechanism of Yangxin decoction on depression model rats based on TLR4/NLRP3 signaling pathway[J].,2025,46(3):306-310.[doi:DOI:10.3969/j.issn.1000-7369.2025.03.004]
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养心汤调控TLR4/NLRP3信号通路治疗抑郁症大鼠机制
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年3期
页码:
306-310
栏目:
基础研究
出版日期:
2025-03-05

文章信息/Info

Title:
Mechanism of Yangxin decoction on depression model rats based on TLR4/NLRP3 signaling pathway
作者:
蒋钰涵; 王 锐; 于钦明
(黑龙江中医药大学,黑龙江 哈尔滨 150040)
Author(s):
JIANG Yuhan; WANG Rui; YU Qinming
(Heilongjiang University of Chinese Medicine,Harbin 150040,China)
关键词:
抑郁症;  养心汤;  Toll样受体4/NOD样受体蛋白3信号通路;  慢性不可预知性刺激;  炎症反应;  抑郁样症状
Keywords:
Depression;  Yangxin decoction;  Toll-like receptor 4/NOD-like receptor protein 3 signaling pathway;  Chronic unpredictable stimulation;  Inflammatory response;  Depression-like symptoms
分类号:
R 256
DOI:
DOI:10.3969/j.issn.1000-7369.2025.03.004
文献标志码:
A
摘要:
目的:探讨养心汤通过TLR4/NLRP3信号通路干预CUMS抑郁模型大鼠的作用机制。方法:50只雄性SD大鼠随机分为对照组、模型组、氟西汀组、养心汤低剂量组、养心汤高剂量组。采用慢性不可预知性刺激建立CUMS大鼠模型,各组大鼠均通过灌胃给药,1次/d,连续灌胃3周。养心汤低剂量组、养心汤高剂量组、氟西汀组大鼠给予相应药物,对照组和模型组给予0.9%氯化钠溶液。糖水偏好、旷场、悬尾和强迫游泳实验检测大鼠抑郁样症状; ELISA法检测大鼠海马组织白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)水平; Hoechst染色检测大鼠海马神经元凋亡程度; RT-PCR和Western blot法检测大鼠脑组织通路因子TLR4、NLRP3与Caspase-1的表达。结果:与对照组相比,模型组大鼠蔗糖偏好、旷场、悬尾、强迫游泳实验结果均呈抑郁样症状。海马组织中IL-1β、IL-18水平升高,神经元出现大量凋亡,TLR4、NLRP3与Caspase-1蛋白与mRNA表达上升,差异有统计学意义(P<0.01)。与模型组相比,养心汤低剂量组、养心汤高剂量组、氟西汀组大鼠抑郁样症状得到显著改善,IL-1β、IL-18降低,差异有统计学意义(均P<0.05)。与模型组相比,养心汤低剂量组、养心汤高剂量组、氟西汀组大鼠海马神经元凋亡得到改善,TLR4、NLRP3、Caspase-1蛋白与mRNA的表达降低,差异有统计学意义(均P<0.05)。结论:养心汤可有效改善CUMS大鼠抑郁样行为,其机制可能与调控TLR4/NLRP3信号通路、缓解炎症反应有关。
Abstract:
Objective:To investigate effect of Yangxin decoction on CUMS depressive model rats by regulating TLR4/NLRP3 signaling pathway.Methods:Fifty male SD rats were randomly divided into control group,model group,fluoxetine group and Yangxin decoction low-dose and high-dose groups.CUMS rat model were established by chronic unpredictability stimulation.All rats in each group were given corresponding drug by intragastrial administration.Yangxin decoction low-dose and high-dose group and fluoxetine group were given corresponding drug,and the control group and model group were given normal saline by intragastrial administration once a day for 3 weeks.Depression-like symptoms were detected by sugar water preference,open field,tail suspension and forced swimming.The levels of interleukin-1β(IL-1β)and interleukin-18(IL-18)in rat hippocampus were detected by ELISA.Hoechst staining were used to detect apoptosis of hippocampal neurons.The expression of pathway factors TLR4,NLRP3 and Caspase-1 were detected by RT-PCR and Western blot.Results:Compared with the control group,the model group showed significant depression-like symptoms in sucrose preference,open field,tail suspension and forced swimming tests.The levels of IL-1β and IL-18 in hippocampus were significantly increased,difference statistically significant(all P<0.01),and a large number of neurons suffered apoptosis.The protein and mRNA expressions of TLR4,NLRP3 and Caspase-1 were significantly increased(all P<0.01).Compared with the model group,depression-like symptoms were significantly improved in Yangxin decoction low dose,high dose and fluoxetine groups,IL-1β and IL-18 levels were decreased,difference statistically significant(all P<0.05),and hippocampal neuron apoptosis were improved.The protein and mRNA expressions of TLR4,NLRP3 and Caspase-1 were significantly decreased,difference statistically significant(all P<0.05).Conclusion:Yangxin decoction can effectively improve depression-like behavior in CUMS rats,and its mechanism may be related to regulating TLR4/NLRP3 signaling pathway and alleviating inflammatory response.

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备注/Memo

备注/Memo:
[基金项目]国家自然科学基金资助项目(82074271)
更新日期/Last Update: 2025-03-10