[1]何卫才,姚安梅,孟凯丽,等.骨碎补抗骨质疏松成分柚皮苷与钙离子促成骨活性机制研究[J].陕西中医,2026,(2):183-189.[doi:DOI:10.3969/j.issn.1000-7369.2026.02.007]
　HE Weicai,YAO Anmei,MENG Kaili,et al.Study on mechanism of osteogenic activity of anti-osteoporosis component naringin from Drynaria fortunei and calcium ions[J].,2026,(2):183-189.[doi:DOI:10.3969/j.issn.1000-7369.2026.02.007]
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骨碎补抗骨质疏松成分柚皮苷与钙离子促成骨活性机制研究

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2026年2期
页码:
183-189
栏目:
基础研究
出版日期:
2026-02-05

文章信息/Info

Title:
Study on mechanism of osteogenic activity of anti-osteoporosis component naringin from Drynaria fortunei and calcium ions
作者:
何卫才1; 姚安梅1; 孟凯丽2; 肖超妮2; 李开飞3
（1.陕西省肿瘤医院，陕西 西安 710061；2.西北大学生命科学学院，陕西 西安710069；3.陕西省森工医院，陕西 西安710300）
Author(s):
HE Weicai1; YAO Anmei1; MENG Kaili2; XIAO Chaoni2; LI Kaifei3
(1.Shaanxi Provincial Cancer Hospital,Xi’an 710061,China；2.College of Life Sciences,Northwest University,Xi’an 710069,China；3.Sengong Hospital of Shaanxi Province,Xi’an 710300,China)
关键词:
骨质疏松; 骨碎补; 柚皮苷; 钙离子; 成骨细胞
Keywords:
Osteoporosis; Drynaria fortunei; Naringin; Calcium; Osteoblast
分类号:
R 282
DOI:
DOI:10.3969/j.issn.1000-7369.2026.02.007
文献标志码:
A
摘要:
目的：评价骨碎补抗骨质疏松成分柚皮苷联合钙离子对MC3T3-E1成骨细胞的分化矿化作用。方法：采用高效液相色谱和质谱技术分析中药骨碎补的化学成分。应用CCK-8法测定不同受试药物对成骨细胞存活率的影响。不同受试药物处理细胞14 d后，采用试剂盒测试碱性磷酸酶（ALP）活性。不同受试药物处理细胞21 d后用茜素红对细胞染色，观测细胞中矿化结节数量。采用Western blot分析成骨细胞内Wnt/β-catenin信号通路相关蛋白质的表达情况。结果：高效液相色谱和质谱检测分析发现，柚皮苷是骨碎补中含量最高的化学成分。CCK-8实验结果表明，当柚皮苷浓度为2.5~5.0 μmol/L柚皮苷具有显著促细胞增殖效应，且当2.0 mmol/L CaCl2存在时对细胞的生长具有保护作用。与单独给药相比，5.0 μmol/L柚皮苷与2.0 mmol/L CaCl2联合用药能够显著增强碱性磷酸酶活性（P＜0.05），且柚皮苷与CaCl2联合用药组的矿化结节数量多于单独给药组，这表明两者具有协同促进成骨细胞的分化与矿化作用。与药物单用组相比，5.0 μmol/L柚皮苷与2.0 mmol/L CaCl2联合给药组细胞Wnt3a和β-catenin蛋白质的表达量显著增加（P＜0.05），推断两者协同促成骨活性与激活Wnt/β-catenin 信号通路有关。结论：骨碎补抗骨质疏松成分柚皮苷联合钙离子干预能够有效刺激成骨细胞增殖并促进分化成熟，为骨质疏松症防治提供中药活性成分及矿物质协同治疗策略。
Abstract:
Objective:The effect of osteoporosis-resistant component naringin from Drynaria fortunei combined with calcium ions on differentiation and mineralization of MC3T3-E1 osteoblasts was evaluated.Methods:The chemical components of Drynaria fortunei were analyzed by high-performance liquid chromatography and mass spectrometry.The CCK-8 method was used to determine the effect of different test drugs on the survival rate of osteoblasts.After treating the cells with different test drugs for 14 days,the activity of alkaline phosphatase was tested by a kit.After treating the cells with different test drugs for 21 days,the cells were stained with alizarin red to observe the number of mineralized nodules.Western blot was used to analyze the expression of proteins related to the Wnt/β-catenin signaling pathway in osteoblasts.Results:High-performance liquid chromatography and mass spectrometry detection and analysis revealed that naringin was the most abundant chemical component in Drynaria fortunei.The CCK-8 experiment results showed that the survival rate of osteoblasts reached the highest region when the concentration of naringin was 2.5~5.0 μmol/L,and the presence of 2.0 mmol/L CaCl2 had a protective effect on cell growth.Compared with single drug administration,the combination of 5.0 μmol/L naringin and 2.0 mmol/L CaCl2 significantly enhanced the activity of alkaline phosphatase(P＜0.05),and the number of mineralized nodules in the naringin and CaCl2 combination group was higher than that in the single drug administration group,indicating that the two have a synergistic effect in promoting the differentiation and mineralization of osteoblasts.Compared with the single drug administration groups,the expression levels of Wnt3a and β-catenin proteins in the osteoblasts of the 5.0 μmol/L naringin and 2.0 mmol/L CaCl2 combination administration group were increased，difference statistically significant (P＜0.05),suggesting that the synergistic promotion of osteogenic activity and activation of the Wnt/β-catenin signaling pathway were related.Conclusion:The intervention of osteoporosis-resistant component naringin from Drynaria fortunei combined with calcium ions can effectively stimulate the proliferation of osteoblasts and promote their differentiation and maturation,providing a strategy for the synergistic treatment of osteoporosis with traditional Chinese medicine active components and minerals.

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备注/Memo

备注/Memo:
陕西省重点研发计划项目(2024SF-ZDCYL-03-27)；北京医卫健康公益基金会资助项目（YWJKJJHKYJJ-2H25014）
更新日期/Last Update: 2026-02-09