[1]钱元霞,汤玲珺,钱 祯,等.四逆散对慢性萎缩性胃炎癌前病变大鼠的作用及机制研究*[J].陕西中医,2019,(8):990-994.
　QIAN Yuanxia,TANG Lingjun,QIAN Zhen,et al.The effect of Sinisan on the rats of precancerous lesion of chronic atrophic gastritis and its mechanism[J].,2019,(8):990-994.
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四逆散对慢性萎缩性胃炎癌前病变大鼠的作用及机制研究*
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2019年8期
页码:
990-994
栏目:
基础研究
出版日期:
2019-08-05

文章信息/Info

Title:
The effect of Sinisan on the rats of precancerous lesion of chronic atrophic gastritis and its mechanism
文章编号:
DOI:10.3969/j.issn.1000-7369.2019.08.002
作者:
钱元霞1; 汤玲珺1; 钱 祯1; 黄晓凡1; 高 静2; 徐卫东2△
1. 江苏省镇江市中西医结合医院药剂科（镇江 212000）；2.江苏大学药学院(镇江 212013）
Author(s):
QIAN Yuanxia; TANG Lingjun; QIAN Zhen; et al.
Department of Pharmacy, Zhenjiang Hospital of Chinese Traditional and Western Medicine（ Zhenjiang 212000)
关键词:
四逆散; 慢性萎缩性胃炎; 癌前病变; 增殖; 凋亡; 大鼠
Keywords:
Key words Sinisan; Chronic atrophic gastritis; Precancerous lesions; Proliferation; Apoptosis; Rats
分类号:
R573.32
文献标志码:
A
摘要:
摘 要 目的：研究四逆散对慢性萎缩性胃炎癌前病变大鼠的作用，为更好地应用于临床提供依据。方法：采用MNNG造模法复制胃黏膜上皮异型增生模型，以不同浓度四逆散处理大鼠，观察病变大鼠体征、体重、脏器系数、胃黏膜组织的变化,采用 ELISA法测定PGⅠ、PGⅡ、G-17含量，Western Blot法检测PCNA、Bcl-2、Bax蛋白表达。结果：四逆散高剂量组体重增长最缓，低、中剂量组对大鼠脏器无明显影响，但高剂量组与正常组相比在肾脏系数，与模型组相比在胸腺系数上有一定差异；四逆散中、高剂量组能降低PGⅡ、升高PGR，四逆散高剂量组能升高G-17浓度；四逆散能降低病变大鼠胃黏膜组织PCNA、Bcl-2的表达，升高Bax的蛋白量。结论：四逆散能抑制或逆转慢性萎缩性胃炎癌前病变的发生，可能与调控细胞增殖与凋亡平衡有关。
Abstract:
Abstract Objective：To study the effect of Sinisan on the rats of precancerous lesion of chronic atrophic gastritis,and provide scientific basis for better clinical application. Methods：The model rats of gastric mucosa epithelial hyperplasia were replicated by MNNG,then different concentrations of Sinisan treated them,observed physical signs,body weight,viscera coefficient,gastric mucosal tissue,detected the PG Ⅰ,PG Ⅱ,G-17 content by ELISA,expressions of PCNA,Bcl-2,Bax were examined by Western blot. Results：The group of high dose Sinisan got the slowest pace in weight,low and medium dose of Sinisan had no obvious effect on rat organs,but kidney coefficient of high dose group was different from the control group,thymus coefficient of high dose group was different from the model group. Medium and high dose of Sinisan could decrease PGⅡand increase PGR,high dose group of Sinisan could increase the concentration of G-17；compared with model group,PCNA and Bcl-2 protein decreased with Sinisan treated,Bax took on a uptrend .Conclusion：To some extent,Sinisan can inhibit or reverse the occurrence of precancerous lesions of chronic atrophic gastritis,maybe the regulatory mechanism is related to balance proliferation and apoptosis.

参考文献/References:

[1] 邹文斌,李兆申.中国胃癌发病率及死亡率研究进展[J].中国实用内科杂志,2014,4（34）:408-415.
[2] 房殿春,夏雨亭,吴云林.胃粘膜癌前病变和癌前疾病[M].成都:四川科学技术出版社,2006:189．
[3] 康宜兵.香苏散合四逆散治疗肝胃不和型慢性非萎缩性胃炎临床研究[J].中医临床研究,2015,7(20):24-26.
[4] 柯 彤.四逆散合益气养阴法治疗慢性萎缩性胃炎49例[J].河南中医,2015,35(6):1213-1215.
[5] 张凤武,张 燕.四逆散加味治疗慢性萎缩性胃炎58例[J].中国医药学报,2000,15(4):79-80.
[6] 国家食品药品监督管理总局.中药新药临床研究指导原则(试行) [M].北京:中国医药科技出版社，2002.
[7] 弓淑珍,侯丽颖.刘友章治疗胃癌前病变经验[J].河北中医,2009,11（31）:1607.
[8] 郑保平.慢性萎缩性胃炎及其癌前病变的病机和证治探讨[J].江苏中医药,2010,22(1)：17-18.
[9] 周 晶,黄柳向,喻 斌,等.MNNG诱导胃癌前病变模型的探讨[J].中国中西医结合消化杂志,2016,24(11):888-890.
[10] 乔正梅,王登峰,赵秋剑,等.血清胃蛋白酶原、胃泌素-17在胃癌及胃癌前病变的诊断价值[J].中国卫生检验杂志,2017,27(2):198-200.
[11] Shafaghi A,Mansourghanaei F,Joukar F,et al. Serum gastrin and the pepsinogen I/II ratio as markers for diagnosis of premalignant gastric lesions[J]. Asian Pacific Journal of Cancer Prevention Apjcp,2013,14(6):3931-3936. 
[12] Loong TH,Soon NC,Nrk NM,et al. Serum pepsinogen and gastrin-17 as potential biomarkers for pre-malignant lesions in the gastric corpus[J]. Biomed Rep,2017,7(5):460-468.
[13] Kang JM,Kim N,Yoo JY,et al. The role of serum pepsinogen and gastrin test for the detection of gastric cancer in Korea[J]. Helicobacter,2010,13(2):146-156.
[14] Hall PA,Levison DA,Woods AL,et al. Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections：an index of cell proliferation with evidence of deregulated expression in some neoplasms[J]. Journal of Pathology,1990,162(4):285.
[15] 刘 润,高海燕,韩淑琴,等.p27蛋白及PCNA在慢性胃病及胃癌患者胃黏膜中的表达及临床意义[J]. 河北医药,2018,40(4):506-509.
[16] Wolter KG,Hsu YT,Smith CL,et al. Movement of Bax from the Cytosol to Mitochondria during Apoptosis[J]. Journal of Cell Biology,1997,139(5):1281-1292.
[17] Desagher S,Martinou JC. Mitochondria as the central control point of apoptosis[J]. Trends in Cell Biology,2000,10(9):369.
[18] 朱玉侠,赵明星,姜登鸽,等.抑癌基因p53、凋亡抑制基因Bcl-2、促凋亡基因Bax在胃癌及癌前病变中的表达[J]. 胃肠病学和肝病学杂志,2016,25(9):1040-1043.

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备注/Memo

备注/Memo:
*江苏省镇江市科学技术局重点研发计划项目（SH2016050）
更新日期/Last Update: 2019-08-15