[1]刘 智,李巧慧,唐 菲,等.清肺抗敏合剂下调RORγt及SOCS1/3表达改善哮喘小鼠炎症反应机制研究[J].陕西中医,2023,(8):1010-1014,1026.[doi:DOI:10.3969/j.issn.1000-7369.2023.08.004]
　LIU Zhi,LI Qiaohui,TANG Fei,et al.Mechanism of Qingfei Kangmin mixture down-regulating RORγt and SOCS1/3 expression to improve inflammatory response in asthmic mice[J].,2023,(8):1010-1014,1026.[doi:DOI:10.3969/j.issn.1000-7369.2023.08.004]
点击复制

清肺抗敏合剂下调RORγt及SOCS1/3表达改善哮喘小鼠炎症反应机制研究
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年8期
页码:
1010-1014,1026
栏目:
基础研究
出版日期:
2023-08-05

文章信息/Info

Title:
Mechanism of Qingfei Kangmin mixture down-regulating RORγt and SOCS1/3 expression to improve inflammatory response in asthmic mice
作者:
刘 智; 李巧慧; 唐 菲; 谢辉辉; 杨 蕾
(衡水市人民医院,河北 衡水 053099)
Author(s):
LIU Zhi; LI Qiaohui; TANG Fei; XIE Huihui; YANG Lei
(Hengshui People's Hospital,Hengshui 053099,China)
关键词:
哮喘;  呼吸道合胞病毒;  炎症反应;  清肺抗敏合剂;  维甲酸孤儿核受体γt;  信号传导抑制因子1/3
Keywords:
Asthma;  Respiratory syncytial virus;  Inflammation response;  Qingfei Kangmin mixture;  RORγt;  SOCS1/3
分类号:
R 256.12
DOI:
DOI:10.3969/j.issn.1000-7369.2023.08.004
文献标志码:
A
摘要:
目的:研究清肺抗敏合剂下调维甲酸孤儿核受体γt(RORγt)及信号传导抑制因子1/3(SOCS1/3)表达改善呼吸道合胞病毒(RSV)诱发哮喘小鼠炎症反应的影响。方法:将BALB/c小鼠随机分为对照组、模型组、地塞米松组和清肺抗敏合剂低、中、高剂量组。通过RSV诱发、白蛋白致敏建立哮喘小鼠模型。末次激发后24 h,吸入乙酰胆碱(Mch)测定气道的相对阻力; 制备肺泡灌洗液(BALF),用酶联免疫吸附法(ELISA)测定BALF中CD31、免疫球蛋白E(IgE)、白介素-4(IL-4)、肿瘤坏死因子-α(TNF-α)水平,采用比色法测定髓过氧化物酶(MPO)含量; 计数炎症细胞。采用RT-PCR法检测肺组织中RORγt mRNA水平。采用qPCR法测定肺组织中SOCS1/3 mRNA表达水平。结果:地塞米松组及清肺抗敏合剂不同剂量组小鼠吸入梯度浓度的Mch后气道阻力与模型组比较显著降低(均P<0.05); 地塞米松和不同剂量清肺抗敏合剂均可以改善小鼠肺组织的病理学损伤,缓解炎性细胞浸润。地塞米松组及清肺抗敏合剂不同剂量组小鼠病理半定量评分显著低于模型组(均P<0.05),且随着剂量的增加评分降低。与模型组比较,地塞米松组及清肺抗敏合剂不同剂量组小鼠中性粒细胞、淋巴细胞、嗜酸性粒细胞和巨噬细胞数量显著降低(均P<0.05),小鼠BALF中CD31、IgE、IL-4、MPO和TNF-α含量显著降低(均P<0.05),肺组织RORγt及SOCS1/3 mRNA表达水平显著降低(均P<0.05)。结论:清肺抗敏合剂可以通过下调RORγt及SOCS1/3 mRNA表达改善呼吸道合胞病毒诱发哮喘小鼠炎症反应。
Abstract:
Objective:To study the effect of Qingfei Kangmin mixture down-regulating the expression of RORγt and SOCS1/3 to improve the inflammatory response of asthmatic mice induced by respiratory syncytial virus.Methods:BALB/c mice were randomly divided into control group,model group,dexamethasone group and low,medium and high dose groups of Qingfei Kangmin mixture.Asthma mouse model was established by respiratory syncytial virus(RSV)induction and albumin sensitization.Twenty-four hours after the last excitation,the relative resistance of airway was measured after inhaling acetylcholine(Mch).Mice were anesthetized and broncho-alveolar lavage fluid(BALF)was prepared.The contents of CD31,IgE,IL-4 and TNF-α in BALF were determined by enzyme-linked immunosorbent assay(ELISA),and myeloperoxidase(MPO)was determined by colorimetry,and inflammatory cells were counting.The level of RORγt mRNA in lung tissue was detected by RT-PCR.The expression level of SOCS1/3 mRNA in lung tissue was determined by qPCR method.Results:Compared with the model group,the airway resistance of dexamethasone group and Qingfei Kangmin mixture group with different doses decreased significantly after inhaling Mch with gradient concentration(all P<0.05).Dexamethasone and different doses of Qingfei Kangmin mixture can improve the pathological injury of mouse lung tissue and relieve inflammatory cell infiltration.The semi-quantitative scores of dexamethasone group and Qingfei Kangmin mixture group were significantly lower than those of model group(all P<0.05),and the score decreased with increasing dose.Compared with model group,the number of neutrophils,lymphocytes,eosinophils and macrophages in dexamethasone group and different doses of Qingfei Kangmin mixture group were significantly decreased(all P<0.05),the contents of CD31,IgE,IL-4,MPO and TNF-α in BALF were significantly decreased(all P<0.05),the expression levels of RORγt and SOCS1/3 mRNA in lung tissue were significantly decreased(all P<0.05).Conclusion:Qingfei Kangmin mixture can improve the inflammatory response of respiratory syncytial virus-induced asthma mice by down-regulating the expression of RORγt and SOCS1/3 mRNA.

参考文献/References:

[1] 王晶波,熊丽琼.中医药治疗哮喘的研究概述[J].辽宁中医杂志,2022,49(4):218-220.
[2] 王松江.RORγt及SOCS1/3在哮喘小鼠肺组织中的表达及意义[D].开封:河南大学,2017.
[3] Britton GJ,Contijoch EJ,Mogno I,et al.Microbiotas from humans with inflammatory bowel disease alter the balance of gut Th17 and RORγt+ regulatory T cells and exacerbate colitis in mice[J].Immunity,2019,50(1):212-224.
[4] Mielke LA,Yang L,Clemens EB,et al.TCF-1 limits the formation of Tc17 cells via repression of the MAF-RORγt axis[J].Journal of Experimental Medicine,2019,216(7):1682-1699.
[5] Hedl M,Sun R,Huang C,et al.STAT3 and STAT5 signaling thresholds determine distinct regulation for innate receptor-induced inflammatory cytokines,and STAT3/STAT5 disease variants modulate these outcomes[J].Journal of Immunology,2019,203(12):3325-3338.
[6] 陈 迪,李建保,王 霞,等.基于“肺阳虚-痰瘀互结”探索儿童哮喘的发病机制[J].中国中医基础医学杂志,2020,26(8):1068-1069,1113.
[7] 严春霞,何国产,闻人庆,等.解毒清肺合剂对肺炎支原体感染大鼠肺组织NF-κB和p38 MAPK通路的影响[J].中国病理生理杂志,2019,35(5):166-172.
[8] 郭钊明,黄楚栓,刘建博,等.难治性哮喘的中医辨治思考[J].中华中医药杂志,2019,34(7):2928-2931.
[9] Xuan X,Sun Z,Yu C,et al.Network pharmacology-based study of the protective mechanism of conciliatory anti-allergic decoction on asthma[J].Allergologia Immunopathologia,2020,48(5):441-449.
[10] Li M,Zheng W,Zhang C,et al.Chuankezhi injection for asthma:Protocol of a systematic review and meta-analysis[J].Medicine,2019,98(33):e16630.
[11] 刘传华.麻杏石甘汤在临床治疗小儿支气管肺炎中的应用效果及对炎症指标的影响[J].心理月刊,2020,15(3):197.
[12] Patel KR.Effect of prostaglandin F2 alpha on lung mechanics in extrinsic asthma[J].Postgraduate Medical Journal,2019,52(607):275-278.
[13] Michalovich D,Rodriguez-Perez N,Smolinska S,et al.Obesity and disease severity magnify disturbed microbiome-immune interactions in asthma patients[J].Nature Communications,2019,10(1):5711.
[14] Spinozzi F,Fizzotti M,Agea E,et al.Defective expression of Fas messenger RNA and Fas receptor on pulmonary T cells from patients with asthma[J].Annals of Internal Medicine,2019,128(5):363-369.
[15] Guttman-Yassky E,Renert-Yuval Y,Bares J,et al.Phase 2a randomized clinical trial of dupilumab(anti-IL-4Rα)for alopecia areata patients[J].Allergy,2022,77(3):897-906.
[16] Utsunomiya T,Hibino A,Taniguchi K,et al.Factors contributing to symptom duration and viral reduction in outpatient children with respiratory syncytial virus infection[J].The Pediatric Infectious Disease Journal,2020,39(8):678-683.
[17] Blanken MO,Frederix GW,Ungar WJ,et al.Correction to:Cost-effectiveness of rule-based immunoprophylaxis against respiratory syncytial virus infections in preterm infants[J].European Journal of Pediatrics,2020,179(2):355.
[18] Mccarthy K,Moore E,Butler K,et al.OC29 respiratory syncytial virus hospital admission and outcome in ireland among infants born less than 32 weeks gestation in the era of changing palivizumab prescription guidelines[J].Archives of Disease in Childhood,2019,104(3):12.
[19] Xu M,Sun S,Ge J,et al.Bupleurum chinense polysaccharide improves LPS-induced senescence of RAW264.7 cells by regulating the NF-κB signaling pathway[J].Evidence-based Complementary and Alternative Medicine,2020,2020(1):1-10.
[20] Tian Y,Han C,Wei Z,et al.SOX-5 activates a novel RORγt enhancer to facilitate experimental autoimmune encephalomyelitis by promoting Th17 cell differentiation[J].Nature Communications,2021,12(1):481.
[21] Cole S,Maroof A.P089 mucosal-associated invariant T(MAIT)-cell-derived IL-17A and IL-17F production is IL-23-independent and biased towards IL-17F[J].Annals of the Rheumatic Diseases,2019,78(S1):38-39.
[22] Saint-Germain E,Lian M,Huot G,et al.Phosphorylation of SOCS1 inhibits the SOCS1-p53 tumor suppressor axis[J].Cancer Research,2019,79(13):3306-3319.
[23] Shi M,Lin Z,Ye L,et al.Estrogen receptor-regulated SOCS3 modulation via JAK2/STAT3 pathway is involved in BPF-induced M1 polarization of macrophages[J].Toxicology,2020,433(9):152404.
[24] 邱 磊,杜学航,张少言,等.基于文献及BK算法探讨突发急性呼吸道传染病的中药复方用药规律[J].中国中医药信息杂志,2020,27(3):84-88.

相似文献/References:

[1]李艳,曾晓翠.加味射干麻黄汤对小儿咳嗽变异性哮喘的疗效观察[J].陕西中医,2016,(10):1311.
[2]伍 磊.紫苏子汤对哮喘小鼠TNFα、白细胞介素8、白介素1β表达的影响*[J].陕西中医,2019,(2):152.
　WU Lei..Effects of Zisuzi decoction on expression of TNF α，interleukin 8 and interleukin 1 β in asthmatic mice[J].,2019,(8):152.
[3]王玉梅,袁向东,李四保.止咳平喘糖浆联合异丙托溴铵治疗哮喘疗效及对患儿肺功能和内环境的影响*[J].陕西中医,2019,(4):428.
　WANG Yumei,YUAN Xiangdong,LI Sibao..Curative effect of cough relieving asthma syrup combined with ipratropium bromide on children with asthma and its influence on pulmonary function and internal environment[J].,2019,(8):428.
[4]汪 伟,赵跃萍,张亚明.补中益气方联合针灸治疗哮喘疗效及对患儿大气道功能相关指标的影响*[J].陕西中医,2020,(11):1611.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.025]
　WANG Wei,ZHAO Yueping,ZHANG Yaming..Effect of Buzhong Yiqi recipe combined with acupuncture and moxibustion on therapeutic effect and changes of large airway function indexes in children with asthma[J].,2020,(8):1611.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.025]
[5]陈训春,李名兰,潘碧云,等.白芥子散对哮喘大鼠免疫稳态重建及RhoA基因表达的影响[J].陕西中医,2021,(7):836.[doi:DOI:10.3969/j.issn.1000-7369.2020.07.005]
　CHEN Xunchun,LI Minglan,PAN Biyun,et al.Immune steady-state reconstruction of sputum drug Baijiezi powder on asthmatic rats and its effect on RhoA gene expression[J].,2021,(8):836.[doi:DOI:10.3969/j.issn.1000-7369.2020.07.005]
[6]刘 健,王佳丽,曹慧丹,等.补肺颗粒对哮喘小鼠气道重塑的影响及机制研究[J].陕西中医,2022,(5):559.[doi:DOI:10.3969/j.issn.1000-7369.2022.05.004]
　LIU Jian,WANG Jiali,CAO Huidan,et al.Mechanism of Bufei granule inhibiting airway remodeling in asthmatic mice[J].,2022,(8):559.[doi:DOI:10.3969/j.issn.1000-7369.2022.05.004]
[7]陆振瑜,庄 承.益气补肾止喘汤联合布地奈德治疗小儿哮喘疗效研究[J].陕西中医,2022,(6):728.[doi:DOI:10.3969/j.issn.1000-7369.2022.06.012]
　LU Zhenyu,ZHUANG Cheng.Effect research on Yiqi Bushen Zhichuan decoction combined with budesonide treatment of children with asthma[J].,2022,(8):728.[doi:DOI:10.3969/j.issn.1000-7369.2022.06.012]
[8]李 星,田春燕,王丽洁,等.李竹英教授治疗哮喘经验[J].陕西中医,2022,(7):936.[doi:DOI:10.3969/j.issn.1000-7369.2022.07.030]
　LI Xing,TIAN Chunyan,WANG Lijie,et al.Professor LI Zhuying's experience in treating asthma[J].,2022,(8):936.[doi:DOI:10.3969/j.issn.1000-7369.2022.07.030]
[9]孙境孺,李竹英.NF-κB信号通路在肺部疾病中的作用研究进展及中医药干预现状[J].陕西中医,2022,(7):972.[doi:DOI:10.3969/j.issn.1000-7369.2022.07.039]
　SUN Jingru,LI Zhuying.Research progress of role of NF-κB signaling pathway in lung diseases and the current situation of traditional Chinese medicine intervention[J].,2022,(8):972.[doi:DOI:10.3969/j.issn.1000-7369.2022.07.039]
[10]梁 嫄,于海洋,司秀影,等.中药调控Notch通路治疗哮喘作用机制研究进展[J].陕西中医,2022,(7):975.[doi:DOI:10.3969/j.issn.1000-7369.2022.07.040]
　LIANG Yuan,YU Haiyang,SI Xiuying,et al.Research progress on mechanism of traditional Chinese medicine regulating Notch pathway in treating asthma[J].,2022,(8):975.[doi:DOI:10.3969/j.issn.1000-7369.2022.07.040]

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81704118); 河北省卫生健康委员会医学科学研究项目(20200247)
更新日期/Last Update: 2023-08-10