[1]张卫丽,张卫红,刘海立,等.基于ox-LDL信号通路探讨桑芪滋阴补肾汤对人脐静脉血管内皮细胞的影响及其作用机制[J].陕西中医,2023,(9):1193-1197.[doi:DOI:10.3969/j.issn.1000-7369.2023.09.007]
　ZHANG Weili,ZHANG Weihong,LIU Haili,et al.The effect of ox-LDL signaling pathway on the occurrence and development of carotid atherosclerosis in hypertension under the regulation of Sangqi Ziyin Bushen decoction and its mechanism[J].,2023,(9):1193-1197.[doi:DOI:10.3969/j.issn.1000-7369.2023.09.007]
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基于ox-LDL信号通路探讨桑芪滋阴补肾汤对人脐静脉血管内皮细胞的影响及其作用机制
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年9期
页码:
1193-1197
栏目:
基础研究
出版日期:
2023-09-05

文章信息/Info

Title:
The effect of ox-LDL signaling pathway on the occurrence and development of carotid atherosclerosis in hypertension under the regulation of Sangqi Ziyin Bushen decoction and its mechanism
作者:
张卫丽1; 张卫红2; 刘海立1; 张文静1 ; 曹 岩1
(1.唐山市中医医院心血管内科,河北 唐山 063000; 2.唐山市丰润区中医医院中风一科,河北 唐山 064000)
Author(s):
ZHANG Weili; ZHANG Weihong; LIU Haili; ZHANG Wenjing; CAO Yan
(Cardiovascular Department of Tangshan Traditional Chinese Medicine Hospital,Tangshan 063000,China)
关键词:
高血压;  颈动脉粥样硬化;  桑芪滋阴补肾汤;  细胞焦亡;  ox-LDL信号通路;  作用机制
Keywords:
High blood pressure;  Carotid atherosclerosis;  Sangqi Ziyin Bushen decoction;  Cell scorch death;  ox-LDL signaling pathway;  Mechanism of action
分类号:
R 544.1
DOI:
DOI:10.3969/j.issn.1000-7369.2023.09.007
文献标志码:
A
摘要:
目的:探讨桑芪滋阴补肾汤对高血压颈动脉粥样硬化发生和发展的影响及其作用机制。 方法:采用ox-LDL诱导HUVEC细胞建立血管内皮细胞损伤模型,分为空白对照组、模型组、高浓度桑芪滋阴补肾汤组、中浓度桑芪滋阴补肾汤组、低浓度桑芪滋阴补肾汤组和阳性药(阿托伐他汀)组。采用CCK8法检测各组细胞存活率; 采用RT-PCR法检测各组细胞中VEGFA的mRNA表达水平; 采用WB法检测各组细胞中NLRP3和Caspase-1的蛋白表达情况; 采用ELISA法检测肺泡灌洗液中IL-6和TNF-α水平。 结果:与空白对照组比较,模型组细胞存活率降低,细胞中VEGFA的mRNA表达水平降低,NLRP3和Caspase-1的蛋白表达水平升高,细胞上清液中IL-6、TNF-α的含量升高(P<0.05); 与模型组比较,低、中、高浓度桑芪滋阴补肾汤组细胞存活率升高,细胞中VEGFA的mRNA表达水平升高,NLRP3和Caspase-1的蛋白表达水平降低,细胞上清液中IL-6、TNF-α的含量降低,升高或降低水平与药物浓度呈正相关,差异具有统计学意义(P<0.05); 与阳性药组比较,高浓度桑芪滋阴补肾汤组细胞存活率、细胞中VEGFA的mRNA表达水平和NLRP3和Caspase-1的蛋白表达水平均无统计学差异(P>0.05)。 结论:桑芪滋阴补肾汤可以对高血压颈动脉粥样硬化发挥血管保护作用,其作用机制可能与通过抑制ox-LDL诱导细胞焦亡通路有关。
Abstract:
Objective:To explore the effect and mechanism of Sangqi Ziyin Bushen decoction on the occurrence and development of hypertensive carotid atherosclerosis.Methods:HUVEC cells were induced by ox-LDL to establish vascular endothelial cell injury model,which were divided into control group,model group,high concentration Sangqi Ziyin Bushen decoction group,medium concentration Sangqi Ziyin Bushen decoction group,low concentration Sangqi Ziyin Bushen decoction group and positive drug(atorvastatin)group.The cell survival rate of each group was detected by CCK8 method.The mRNA expression of VEGFA in each group of cells was detected by RT-PCR method.The protein expression of NLRP3 and Caspase-1 in cells of each group was detected by Western blotting method.Detection of IL-6 and TNF-α in bronchoalveolar lavage fluid by ELISA method.Results:Compared with the blank control group,the cell survival rate of the model group decreased,the mRNA expression of VEGFA decreased,the protein expression of NLRP3 and Caspase-1 increased,and the contents of IL-6 and TNF-α in the supernatant increased(P<0.05).Compared with the model group,the cell survival rate of Sangqi Ziyin Bushen decoction group increased in a dose-dependent manner,the mRNA expression of VEGFA in the cells increased,the protein expression levels of NLRP3 and Caspase-1 decreased,and the contents of IL-6 and TNF-α in the supernatant decreased,and they were positively correlated with drug concentration.The data were statistically significant(P<0.05).Compared with the positive drug group,there was no significant difference in cell survival rate,mRNA expression of VEGFA and protein expression of NLRP3 and Caspase-1 in high concentration Sangqi Ziyin Bushen decoction group(P>0.05).Conclusion:Sangqi Ziyin Bushen decoction can improve hypertensive carotid atherosclerosis and play a vascular protective effect,and its mechanism may be related to the inhibition of ox-LDL-induced cell death pathway.

参考文献/References:

[1] 冯金艳,邱丽华.MRI检查对颈动脉粥样硬化斑块的诊断价值研究[J].陕西医学杂志,2019,48(4):467-469.
[2] 杨思进,王承刚,白 雪,等.益气祛风通络法对高血压颈动脉粥样硬化家兔NF-κB与ICAM-1表达的作用研究[J].泸州医学院学报,2014,37(1):47-51.
[3] Cuspidi C,Meani S,Valerio C,et al.Carotid atherosclerosis and cardiovascular risk stratification:Role and costeffecti-neness of echo-Doppler examination in untreated essential hypertensions[J].Blood Press,2006,15:333-339.
[4] 桂明泰,曹 慧,钱 佳,等.加味越鞠丸对高血压患者颈动脉粥样硬化、血脂及炎性因子的影响[J].陕西中医,2022,43(2):181-184.
[5] 郭春霞,陈震霖.中医从心肝肾论治高血压病现代作用机制及优势探讨[J].陕西中医,2020,41(5):650-652,666.
[6] Lin JD,Nishi H,Poles J,et al.Single-cell analysis of fate-mapped macrophages reveals heterogeneity,including stem-like properties,during atherosclerosis progression and regression[J].JCI Insight,2019,4(4):e124574.
[7] Kattoor AJ,Kanuri SH,Mehta JL.Role of ox-LDL and LOX-1 in atherogenesis[J].Curr Med Chem,2019,26(9):1693-1700.
[8] 姚黎洁,张 茜.仙茅苷调控circ0004104对ox-LDL诱导的血管内皮细胞损伤的影响[J].河北医药,2022,44(16):2426-2430.
[9] 刘 琳,柴志勇,刁云辉,等.当归多糖对氧化型低密度脂蛋白诱导的血管内皮细胞损伤的保护作用研究[J].中国临床药理学杂志,2020,36(9):92-95.
[10] 李 霞,邸亚丽,亢小丽,等.阿托伐他汀对氧化型低密度脂蛋白诱导内皮细胞损伤的保护作用[J].中国临床药理学杂志,2021,37(10):1184-1188.
[11] 王丽君,赵 瑜,胡 烨,等.miR-30a对脂多糖诱导人脐静脉血管内皮细胞自噬的影响[J].浙江医学,2021,43(21):2299-2302,2307.
[12] 王亚静,贾宝辉,韩方方,等.人环状RNA_0114428对脂多糖诱导的人肾小管上皮HK-2细胞损伤的作用及其机制[J].吉林大学学报:医学版,2023,49(1):101-109.
[13] 王 君,席新龙,郑明娟,等.健康体检者颈动脉粥样硬化与同型半胱氨酸、血脂、血糖水平相关性研究[J].陕西医学杂志,2018,47(5):600-603.
[14] 李 欣,王彦婷,仇秀莹,等.高血压合并颈动脉粥样硬化患者IMT与CK-MB、hsTnI及CRP水平的相关性[J].分子诊断与治疗杂志,2022,14(12):2159-2162.
[15] 林从娟,郑建清,蔡川奇,等.血压变异性与血浆同型半胱氨酸对高血压患者颈动脉粥样硬化的影响[J].中华高血压杂志,2021,29(12):1238-1244.
[16] 万密密,赵梓楠,李 婷,等.动脉粥样硬化治疗的新思路[J].江苏大学学报:医学版,2021,31(1):1-5.
[17] 赵连友,孙英贤,李玉明,等.高血压合并动脉粥样硬化防治中国专家共识[J].中华高血压杂志,2020,28(2):116-123.
[18] 肖 振,李盛华.激素性股骨头坏死血管新生相关基因的鉴定及其靶向中药成分筛选验证[J].中草药,2023,54(5):1526-1539.
[19] 雷雪丽,王 震.中药天然产物调控内皮细胞防治动脉粥样硬化的研究进展[J].中国动脉硬化杂志,2023,31(1):70-79.
[20] 李宝福,刘柏麟,袁 兴,等.参芍口服液抑制NLRP3炎症小体改善创伤性脑损伤小鼠炎性反应实验研究[J].陕西医学杂志,2022,51(5):552-555,560.
[21] 杨佳乐,沈祥春.灯盏花乙素通过抑制NLRP3/caspase-1信号通路改善LPS+ATP诱导内皮细胞炎症反应和细胞焦亡[J].中国药理学通报,2022,38(8):1196-1201.
[22] Chen R,Sun G,Yang L,et al.Salvianolic acid B protects against doxorubicin induced cardiac dysfunction via inhibition of ER stress mediated cardiomyocyte apoptosis[J].Toxicol Res(Camb),2016,5(5):1335-1345.
[23] Huang HS,Tsai CL,Chang J,et al.Multiplex PCR system for the rapid diagnosis of respiratory virus infection:Systematic review and meta-analysis[J].Clinical Microbiology and Infection,2018,24(10):1055-1063.
[24] 郭美君,李 昊,杜珊珊,等.白细胞介素6和病毒感染与免疫[J].病毒学报,2023,39(1):287-294.
[25] Vasiliy PM,Anton PC,Juan A,et al.Neuraminidase mutations conferring resistance to oseltamivir in influenza A(H7N9)viruses henju Marjuki,Gubareva[J].J Virol,2015,89(10):5419-5426.
[26] Wu Y,Gao F,Qi J,et al.Resistance to mutant group 2 influenza virus neuraminidases of an oseltamivir-zanamivir hybrid inhibitor[J].J Virol,2016,90(23):10693-10700.

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备注/Memo

备注/Memo:
基金项目:河北省医学科学研究重点项目(2018314)
更新日期/Last Update: 2023-09-08