[1]武玉刚,朱玉龙,马红霞.祛风止痉胶囊对支气管哮喘大鼠气道炎症及TRPV1通路的影响[J].陕西中医杂志,2024,(4):463-467.[doi:DOI:10.3969/j.issn.1000-7369.2024.04.006]
　WU Yugang,ZHU Yulong,MA Hongxia.Effect of Qufeng Zhijing capsule on airway inflammation and TRPV1 pathway in bronchial asthma rats[J].,2024,(4):463-467.[doi:DOI:10.3969/j.issn.1000-7369.2024.04.006]
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祛风止痉胶囊对支气管哮喘大鼠气道炎症及TRPV1通路的影响
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《陕西中医》杂志[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年4期
页码:
463-467
栏目:
基础研究
出版日期:
2024-04-05

文章信息/Info

Title:
Effect of Qufeng Zhijing capsule on airway inflammation and TRPV1 pathway in bronchial asthma rats
作者:
武玉刚; 朱玉龙; 马红霞
(新疆医科大学附属中医医院,新疆 乌鲁木齐 830000)
Author(s):
WU Yugang; ZHU Yulong; MA Hongxia
(Xinjiang Medicine University Affiliated Traditional Chinese Medicine Hospital,Urumqi 830000,China)
关键词:
哮喘;  气道炎症;  祛风止痉胶囊;  瞬时受体电位香草酸亚型1;  气道平滑肌;  大鼠
Keywords:
Asthma;  Airway inflammation;  Qufeng Zhijing capsule;  Instantaneous receptor potential channel 1;  Airway smooth muscle;  Rats
分类号:
R 562
DOI:
DOI:10.3969/j.issn.1000-7369.2024.04.006
文献标志码:
A
摘要:
目的:观察祛风止痉胶囊对支气管哮喘大鼠气道平滑肌瞬时受体电位香草酸亚型1(TRPV1)表达以及外周血白介素(IL)-4、IL-13、IL-15、IL-17水平的影响。方法:将60只健康雄性大鼠随机分为正常组、阳性对照组、模型组与低剂量组、中剂量组、高剂量组,每组10只。除正常组外,其余各组大鼠于实验的第1、15天给予10%OVA腹腔注射1 ml以致敏,第15天将致敏的大鼠给予1%OVA溶液进行超声雾化建立哮喘模型。连续干预10 d。采用ELISA法测定大鼠外周血IL-4、IL-13、IL-5、IL-17水平,采用荧光定量PCR及Western blot测定大鼠肺组织TRPV1 mRNA和蛋白的表达。结果:低剂量、中剂量组、高剂量组IL-4、IL-13、IL-5、IL-17水平低于模型组(均P<0.05),高剂量组IL-4、IL-13、IL-5、IL-17水平低于阳性对照组(均P<0.05)。模型组及阳性对照组的TRPV1 mRNA和蛋白表达高于正常组(均P<0.05),模型组的TRPV1 mRNA和蛋白表达高于阳性对照组、低剂量组、中剂量组、高剂量组(均P<0.05)。结论:祛风止痉胶囊可降低哮喘大鼠气道炎症因子水平,抑制哮喘大鼠气道平滑肌TRPV1表达。
Abstract:
Objective:To observe TRPV1 expression in airway smooth muscle and the levels of IL-4,IL-13,IL-5,and IL-17 in peripheral blood of rats with bronchial asthma treated with Qufeng Zhijing capsule.Methods:Sixty healthy male rats were randomly divided into normal group,model group,positive control group,high-dose,medium-dose,and low-dose groups,with 10 rats in each group.Except for the normal group,all other groups were given intraperitoneal injection 10% OVA to sensitize rats on the 1st and 15th days of the experiment.On the 15th day,sensitized rats were given 1% OVA physiological saline for ultrasonic nebulization to establish an asthma model.After 10 consecutive days of intervention,ELISA method was used to measure IL-4,IL-13,IL-5,and IL-17 in peripheral blood of rats.Fluorescence quantitative PCR and Western blot techniques were used to determine the relative expression of TRPV1 mRNA and TRPV1 protein in rat lung tissue.Results:The levels of IL-4,IL-13,IL-5,and IL-17 in the high-dose,medium-dose,and low-dose groups of Qufeng Zhijing capsule were all lower than those in the model group(all P<0.05),while the levels of IL-4,IL-13,IL-5,and IL-17 in the high-does group were lower than those in the positive control group(all P<0.05).The expression of TRPV1 mRNA and protein in model group and positive control group was higher than those in normal group(all P<0.05).The expression of TRPV1 protein and TRPV1 mRNA in the positive control group,low-dose,medium-dose,and high-does groups were lower than those in the model group(all P<0.05).Conclusion:Qufeng Zhijing capsule can reduce airway inflammatory factors and inhibit the expression of TRPV1 in airway smooth muscle of asthmatic rats.

参考文献/References:

[1] REDDEL H K,BACHARIER L B,BATEMAN E D,et al.Global initiative for asthma strategy 2021:Executive summary and rationale for key changes[J].European Respiratory Journal,2022,59(21):1-25.
[2] 朱金凤,支气管哮喘的中医辨治思维与方法研究[J],中国中医基础医学杂志,2016,22(2):284-286.
[3] 胡作为.止哮平喘方对哮喘豚鼠T淋巴免疫功能影响的实验研究[D].武汉:湖北中医学院,2002.
[4] 刘霞,王亚明,陈玲,等.玉屏风口服液合人参五味子汤加减治疗小儿支气管哮喘缓解期肺脾气虚证的研究[J].现代中西医结合杂志,2017,19(32):69-71.
[5] 王珊,程亚伟,陈磊,等.温肺健脾化痰汤配合热敏灸对支气管哮喘慢性持续期痰哮证患者中医证候积分及Th17/Treg平衡的影响[J].陕西中医,2022,43(1):58-61.
[6] 庄鹏晖,刘亮亮,郑国玺,等.支气管哮喘患者血清肿瘤坏死因子-α、白细胞介素-8和嗜酸性粒细胞阳离子蛋白水平变化及意义[J].陕西医学杂志,2020,49(12):1597-1599,1603.
[7] JIANG X W,MA Z P.Theroy dissison on airway remodeling of bronchial asthma[J].TMR Theory and Hypothesis,2021,4(1):431-436.
[8] NAGNSE H,UEKI S,FUJIEDA S,et al.The roles of IL-5 and anti-IL-5 trealement in eosinophilic disease:Asthma,eosinophilic granulomatosis with polyangitis,and eosinophilic chronic rhinosinusitis[J].Allergol Int,2020,69(2):178-186.
[9] OESER K,MAXEINER J,SYMOWSKI C,et al.T cells are the critical source of IL-4/IL-13 in a mouse model of allergic asthma[J].Allergy,2015,70(11):1440-1449.
[10] REN Y F,LI H,XING X H,et al.Preliminary study on pathogenesis of bronchial asthma in children[J].Pediatr Res,2015,77(4):506-510.
[11] 杜丽娟,何成诗,由凤鸣,等.敏咳煎对咳嗽变异性哮喘大鼠Treg/Th17细胞及其相关细胞因子的影响[J].辽宁中医杂志,2017,44(3):601-603.
[12] 张丹丹,刘剑南,孙敏,等.百令胶囊对老年性咳嗽变异性哮喘患者肺功能及IL-17的影响[J].实用老年医学,2016,30(7):586-587.
[13] TAKESHI N.Steroid-resistant asthma and neutrophils[J].Biol Pharm Bull,2020,43(1):31-35.
[14] RAHMAWATIS F,TEVELDE M,KERSTJENS H A M,et al.Pharmacology,rationale for targeting IL-17 in asthma[J].Falgy,2021,8(30):694514.
[15] GOUIN O,HERONDELLE K,LEBONVALLET N,et al.TRPV1 and TRPA1 in cutaneous neurogenic and chronic inflammation:Pro-inflammatory response induced by their activation and their sensitization[J].Protein Cell,2017,8(9):644-661.
[16] RUSSO A F.Overview of neuropeptides:Awakening the senses[J].Headache,2017,5(9):13084.
[17] YIP W,HUGHES M R,LI Y,et al.Butyrate shapes immune cell fate and function in allergic asthma[J].Frontiers in Immunology,2012(62):8453.
[18] SAHOO A,WALI S,NURIEVA R.T helper 2 and T follicular helper cells:Regulation and function of interleukin-4[J].Cytokine and Growth Factor Reviews,2016(30):29-37.
[19] JOON Y C,HWA Y L,JUNG H,et al.TRPV1 blocking alleviates airway inflammation and remodeling in a chronic asthma murine model[J].Allergy Asthma Immunol Res,2018,10(3):216-224.
[20] ELNAGGAR R K,MOAWD S A,ALI S E,et al.Potentialimpacts of acu-TENS in the treatment of adolescent with moderer-ate to sever bronchialasthma:A randomized clinical study[J].Complent Ther Med,2021,57(1):102673.
[21] 高辉,刘辉,韩仙菊.黄芩素上调miR-625-5p表达干预支气管哮喘小鼠的机制研究[J].陕西中医,2022,43(7):838-841.
[22] 白文梅,李风森.祛风止痉散对哮喘风痰哮证患者肺功能、FeNO的影响[D].乌鲁木齐:新疆医科大学,2012.
[23] 顾成娟,刘文科,王涵.蝉蜕、藏青果、胖大海治疗风热咽炎经验——仝小林三味小方撷萃[J].吉林中医药,2020,40(1):15-17.
[24] 程智方,吴俊良,温红娟.《神农本草经》治“惊痫”药物分析[J].长春中医药大学学报,2020,36(2):216-218.
[25] 张秋凤,李薇,吴晓东.气道重构的发病机制与地龙的药理作用[J].医学综述,2018(24):1115-1120.

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备注/Memo

备注/Memo:
基金项目:新疆维吾尔自治区科技厅天山创新团队计划项目(2023D14004); “天山英才”-青年科技拔尖人才项目(2022TSYCCX0028); 新疆医科大学附属中医医院科研创新基金资助项目(ZYY2020ZD02)
更新日期/Last Update: 2024-04-10